EMP2 (NM_001424) Human Untagged Clone

SKU SC108082

EMP2 (untagged)-Human epithelial membrane protein 2 (EMP2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: EMP2
• Synonyms: XMP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108082 sequence for NM_001424 edited (data generated by NextGen Sequencing); ATGTTGGTGCTTCTTGCTTTCATCATCGCCTTCCACATCACCTCTGCAGCCTTGCTGTTC; ATTGCCACCGTCGACAATGCCTGGTGGGTAGGAGATGAGTTTTTTGCAGATGTCTGGAGA; ATATGTACCAACAACACGAATTGCACAGTCATCAATGACAGCTTTCAAGAGTACTCCACG; CTGCAGGCGGTCCAGGCCACCATGATCCTCTCCACCATTCTCTGCTGCATCGCCTTCTTC; ATCTTCGTGCTCCAGCTCTTCCGCCTGAAGCAGGGAGAGAGGTTTGTCCTAACCTCCATC; ATCCAGCTAATGTCATGTCTGTGTGTCATGATTGCGGCCTCCATTTATACAGACAGGCGT; GAAGACATTCACGACAAAAACGCGAAATTCTATCCCGTGACCAGAGAAGGCAGCTACGGC; TACTCCTACATCCTGGCGTGGGTGGCCTTCGCCTGCACCTTCATCAGCGGCATGATGTAC; CTGATACTGAGGAAGCGCAAATAG; ; Clone variation with respect to NM_001424.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001424 unedited; TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCCTCGACTCGCC; CTGCCCTGTGAAAAGTTGGTGCTTCTTGCTTTCATCATCGCCTTCCACATCACCTCTGCA; GCCTTGCTGTTCATTGCCACCGTCGACAATGCCTGGTGGGTAGGAGATGAGTTTTTTGCA; GATGTCTGGAGAATATGTACCAACAACACGAATTGCACAGTCATCAATGACAGCTTTCAA; GAGTACTCCACGCTGCAGGCGGTCCAGGCCACCATGATCCTCTCCACCATTCTCTGCTGC; ATCGCCTTCTTCATCTTCGTGCTCCAGCTCTTCCGCCTGAAGCAGGGAGAGAGGTTTGTC; CTAACCTCCATCATCCAGCTAATGTCATGTCTGTGTGTCATGATTGCGGCCTCCATTTAT; ACAGACAGGCGTGAAGACATTCACGACAAAAACGCGAAATTCTATCCCGTGACCAGAGAA; GGCAGCTACGGCTACTCCTACATCCTGGCGTGGGTGGCCTTCGCCTGCACCTTCATCAGC; GGCATGATGTACCTGATACTGAGGAAGCGCAAATAGAGTTCCGGAGCTGGGTTGCTTCTG; CTGCAGTACAGAATCCACATTCAGATAACCATTTTGTATATAATCATTATTTTTTTGAGG; TTNTTCTAGCAAACGTNATTGTTTCCTTTTAAAGCCAAAAAAAAAAAAANAAANNNANNA; NAANAAAAAANANAAAAAAAANNAAAANAAAAAAAAAAANNAAAAAAAAAAAAAAANACC; CTCATTTTAATTGCCGCCGGTCTATAACTTTTTCTTGACAATCCCCGGGTGGATTCCTGG; GACCCTCCCAGGCCTTCTGCCCCGTGGAAGTGCCCTTCACGGCCCCACCTGGCTAAAAAA; ATAGTGCTCATTTGCGACAAGGGCTATN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001424 unedited; TTTTTTTTTTNNNNNTTNNNTTTTTATTTATTTTTTTTTGAAATTCTTTAGGATAACATA; CGTTTGCTAGNAAAACCTCAAAAATAATGATTTTACAAAAGGTTCATCTGATGTGGATTC; TGTACTGCAGCAGAAGCAACCCAGCTCCGGAACTCTATTTGCGCTTCCTCAGTATCAGGT; ACATCATGCCGCTGATGAAGGTGCAGGCGAAGGCCACCCACGCCAGGATGTAGGAGTAGC; CGTAGCTGCCTTCTCTGGTCACGGGATAGAATTTCGCGTTTTTGTCGTGAATGTCTTCAC; GCCTGTCTGTATAAATGGAGGCCGCATTCATGACACACAGACATGACATTACCTGGATGA; TGGAGGTTAGGACAAACCTCTCTCCCTGCTTCAGGCGGAAGAGCTGGAGCACGAAGATGA; AGAAGGCGATGCAGCAGATAATGGTGGAGAGGATCATGGTGGCCTGGACCGCCTGCAGCG; TGGAGTACTCTTGAAAGCTGTCATTGATGACTGTGCAATTCGTGTTGATGGTACATATTC; TCCAGACATCTGCAAAAAACTCATCTCCTACCCACCCAGCATTGTCGACGGTGGCAATGA; ACAGCAAGGCTTGACAGGTGATGTGGAAGGCGATGATGAAAGCCAGACACCCCAACCTTT; TCACAGTGCACGGCCAAGCCAGGCGACTTCGTGCCCGAATTTCCCGGCCGCCCTATAGTG; AGCCCGGATTACAAAAATTCTGGCGGGTCCACTAAACGAAGCTCTGGTTTATTAAACCTT; CCCACCGTACACGCCCTACCGCCCAATTTGGGTCCACGGGGCCGGGGTATTTACCACATT; TTTGGAAAGTTCCCGTTGTTTCTTGGGGGCCCAAACAAAACTCCCCTATGCCCGTTAATT; GGGCGTGGCAGACTTTGAACATCCCCGGCGAGTANACACGCTTTTGTCACGCCCCATCTG; CCGGACCTCGCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_001424
• Insert Size: 660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001424.3, NP_001415.1
• RefSeq Size: 1484 bp
• RefSeq ORF: 504 bp
• Locus ID: 2013
• UniProt ID: P54851
• Cytogenetics: 16p13.13
• Domains: PMP22_Claudin
• Protein Families: Transmembrane
• Summary: This gene encodes a tetraspan protein of the PMP22/EMP family. The encoded protein regulates cell membrane composition. It has been associated with various functions including endocytosis, cell signaling, cell proliferation, cell migration, cell adhesion, cell death, cholesterol homeostasis, urinary albumin excretion, and embryo implantation. It is known to negatively regulate caveolin-1, a scaffolding protein which is the main component of the caveolae plasma membrane invaginations found in most cell types. Through activation of PTK2 it positively regulates vascular endothelial growth factor A. It also modulates the function of specific integrin isomers in the plasma membrane. Up-regulation of this gene has been linked to cancer progression in multiple different tissues. Mutations in this gene have been associated with nephrotic syndrome type 10 (NPHS10). [provided by RefSeq, Mar 2015]