CD82 (NM_002231) Human Untagged Clone

SKU SC107926

CD82 (untagged)-Human CD82 molecule (CD82), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: CD82
• Synonyms: 4F9; C33; GR15; IA4; KAI1; R2; SAR2; ST6; TSPAN27
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107926 sequence for NM_002231 edited (data generated by NextGen Sequencing); ATGGGCTCAGCCTGTATCAAAGTCACCAAATACTTTCTCTTCCTCTTCAACTTGATCTTC; TTTATCCTGGGCGCAGTGATCCTGGGCTTCGGGGTGTGGATCCTGGCCGACAAGAGCAGT; TTCATCTCTGTCCTGCAAACCTCCTCCAGCTCGCTTAGGATGGGGGCCTATGTCTTCATC; GGCGTGGGGGCAGTCACTATGCTCATGGGCTTCCTGGGCTGCATCGGCGCCGTCAACGAG; GTCCGCTGCCTGCTGGGGCTGTACTTTGCTTTCCTGCTCCTGATCCTCATTGCCCAGGTG; ACGGCCGGGGCCCTCTTCTACTTCAACATGGGCAAGCTGAAGCAGGAGATGGGCGGCATC; GTGACTGAGCTCATTCGAGACTACAACAGCAGTCGCGAGGACAGCCTGCAGGATGCCTGG; GACTACGTGCAGGCTCAGGTGAAGTGCTGCGGCTGGGTCAGCTTCTACAACTGGACAGAC; AACGCTGAGCTCATGAATCGCCCTGAGGTCACCTACCCCTGTTCCTGCGAAGTCAAGGGG; GAAGAGGACAACAGCCTTTCTGTGAGGAAGGGCTTCTGCGAGGCCCCCGGCAACAGGACC; CAGAGTGGCAACCACCCTGAGGACTGGCCTGTGTACCAGGAGGGCTGCATGGAGAAGGTG; CAGGCGTGGCTGCAGGAGAACCTGGGCATCATCCTCGGCGTGGGCGTGGGTGTGGCCATC; ATCGAGCTCCTGGGGATGGTCCTGTCCATCTGCTTGTGCCGGCACGTCCATTCCGAAGAC; TACAGCAAGGTCCCCAAGTACTGA; ; Clone variation with respect to NM_002231.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002231 unedited; GCAGCCCAATTCGCACGAGGCCGACGCCGTCTGAGGCACGAGCGGGTGACGCTGGGCCTG; CAGCGCGGAGCAGAAAGCAGAACCCGCAGAGTCCTCCCTGCTGCTGTGTGGACGACACGT; GGGCACAGGCAGAAGTGGGCCCTGTGACCAGCTGCACTGGTTTCGTGGAAGGAAGCTCCA; TACTGGCGGGATGGGCTCAGCCTGTATCAAAGTCACCAAATACTTTCTCTTCCTCTTCAA; CTTGATCTTCTTTATCCTGGGCGCAGTGATCCTGGGCTTCGGGGTGTGGATCCTGGCCGA; CAAGAGCAGTTTCATCTCTGTCCTGCAAACCTCCTCCAGCTCGCTTAGGATGGGGGCCTA; TGTCTTCATCGGCGTGGGGGCAGTCACTATGCTCATGGGCTTCCTGGGCTGCATCGGCGC; CGTCAACGAGGTCCGCTGCCTGCTGGGGCTGTACTTTGCTTTCCTGCTCCTGATCCTCAT; TGCCCAGGTGACGGCCGGGGCCCTCTTCTACTTCAACATGGGCAAGCTGAAGCAGGAGAT; GGGCGGCATCGTGACTGAGCTCATTCGAGACTACAACAGCAGTCGCGAGGACAGCCTGCA; GGATGCCTGGGACTACGTGCAGGCTCAGGTGAAGTGCTGCGGCTGGGTCAGCTTCTACAA; CTGGACAGACAACGCTGAGCTCATGAATCGCCCTGAGGTCACCTACCCCTGTTCCTGCGA; AGTCAAGGGGGAAGAGGACAACAGCCTTTCTGTGAGGAAGGGCTTCTGCGAGGCCCCCGG; CCACAGGACCCANAGTGGCAACCACCCTGAGGACTGGCCTGTGTACCAGGAAGGCTGCAT; GGGAAAAGTGCAAGCTTGGCTGCAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002231 unedited; GCTCTTAGCGGGCCCCTAGTCTGGAAGTGCAGTAGCACGATCTCAGCTCGCTGCAACCTT; CTCTCCCGGGTTCACACCATTGTGCTGCCTGAGCCTCCCAATAGCTGGGACTACAGGTGC; CCGCCACCGCGCCCGGCTAAATTTTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGT; TAGCCAGGATGGTCTCGATCTCCTGACCTCGTGATCCACCCGCCTCGGCCTCCCAAAGGG; CGGGGAGTACAGGCGTGAGCCACCGCGCCCGGCCCCCTCCTCCCCAATTTTTCATACAGC; TGCCCCTATACAATATACACACCCTTGAGGGCAGGTAGAAGTCCAGCCCACCTGCGCCAG; GGACGCTGTGGGGAGCATTTTTCTCTGCGTTGCCTAAGAGAACCCTGATGGGCGGTGAGC; TGAGGAACCACACAACAGTCACGGCTCAATGCTGGTACCGCCTACGCCAGGAGAGATCGT; TATGTCCTACAAAGCCCTCTACTACCATTTAGGGAGGGCTCCAGGGTCTTCCCACTGCAC; CGGGATGCACGCCCTGCAGACGGAGCCATGGGACATCTCTGGAGCCCCGAGGTTGTGTGT; CACGCCTGCACATGCCATTTTCCCTTCCTCACTTCTTGTTGTTTTTTTTTTGCTTTCCCG; ATGGCCCTCCTCCGCCTATTCGTTTGTCCGGTACTATCCCCCCTTTTTCCTTTTTCGCCC; CTCTCTTTCCTCTCCCTCGCTCCTACTTTGCCCCCCCCGCTCCACCCGTTTTTTCTTTTC; CTCTTCCTCTTTCGGTTCCTTATTTCCGCACTCACGTCCTTCCCTCTCCCTATCCACTTC; TCCCGTCCCCTCTCTTTTCTCTCCCCCATTTATTCTCCCCCCTATTTTTTCCTTTTTCGC; GGCCTCGCCTTTCCTCCCGCCTTTTTCTCTCTTTCACCGCTTTTCATCTCTCTNCTCTTA; TCCTCTTTCTCCACGTCATTCCTACCCACCCACACCACTCTATTTCTTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_002231
• Insert Size: 1680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002231.3, NP_002222.1
• RefSeq Size: 1715 bp
• RefSeq ORF: 804 bp
• Locus ID: 3732
• UniProt ID: P27701
• Cytogenetics: 11p11.2
• Domains: transmembrane4
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: p53 signaling pathway
• Summary: This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes the longer isoform (1).