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CD82 (NM_002231) Human Tagged ORF Clone

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SKU
RC200457
CD82 (Myc-DDK-tagged)-Human CD82 molecule (CD82), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CD82
Synonyms 4F9; C33; GR15; IA4; KAI1; R2; SAR2; ST6; TSPAN27
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200457 representing NM_002231
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCTCAGCCTGTATCAAAGTCACCAAATACTTTCTCTTCCTCTTCAACTTGATCTTCTTTATCCTGG
GCGCAGTGATCCTGGGCTTCGGGGTGTGGATCCTGGCCGACAAGAGCAGTTTCATCTCTGTCCTGCAAAC
CTCCTCCAGCTCGCTTAGGATGGGGGCCTATGTCTTCATCGGCGTGGGGGCAGTCACTATGCTCATGGGC
TTCCTGGGCTGCATCGGCGCCGTCAACGAGGTCCGCTGCCTGCTGGGGCTGTACTTTGCTTTCCTGCTCC
TGATCCTCATTGCCCAGGTGACGGCCGGGGCCCTCTTCTACTTCAACATGGGCAAGCTGAAGCAGGAGAT
GGGCGGCATCGTGACTGAGCTCATTCGAGACTACAACAGCAGTCGCGAGGACAGCCTGCAGGATGCCTGG
GACTACGTGCAGGCTCAGGTGAAGTGCTGCGGCTGGGTCAGCTTCTACAACTGGACAGACAACGCTGAGC
TCATGAATCGCCCTGAGGTCACCTACCCCTGTTCCTGCGAAGTCAAGGGGGAAGAGGACAACAGCCTTTC
TGTGAGGAAGGGCTTCTGCGAGGCCCCCGGCAACAGGACCCAGAGTGGCAACCACCCTGAGGACTGGCCT
GTGTACCAGGAGGGCTGCATGGAGAAGGTGCAGGCGTGGCTGCAGGAGAACCTGGGCATCATCCTCGGCG
TGGGCGTGGGTGTGGCCATCGTCGAGCTCCTGGGGATGGTCCTGTCCATCTGCTTGTGCCGGCACGTCCA
TTCCGAAGACTACAGCAAGGTCCCCAAGTAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200457 representing NM_002231
Red=Cloning site Green=Tags(s)

MGSACIKVTKYFLFLFNLIFFILGAVILGFGVWILADKSSFISVLQTSSSSLRMGAYVFIGVGAVTMLMG
FLGCIGAVNEVRCLLGLYFAFLLLILIAQVTAGALFYFNMGKLKQEMGGIVTELIRDYNSSREDSLQDAW
DYVQAQVKCCGWVSFYNWTDNAELMNRPEVTYPCSCEVKGEEDNSLSVRKGFCEAPGNRTQSGNHPEDWP
VYQEGCMEKVQAWLQENLGIILGVGVGVAIVELLGMVLSICLCRHVHSEDYSKVPKY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002231
ORF Size 801 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002231.4
RefSeq Size 1715 bp
RefSeq ORF 804 bp
Locus ID 3732
UniProt ID P27701
Cytogenetics 11p11.2
Domains transmembrane4
Protein Families Druggable Genome, Transmembrane
Protein Pathways p53 signaling pathway
MW 29.4 kDa
Summary This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. provided by RefSeq, Jul 2008
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"NM_002231" in other vectors (7)
SKU Description Size Price
RC200457L1 Lenti ORF clone of Human CD82 molecule (CD82), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC200457L2 Lenti ORF clone of Human CD82 molecule (CD82), transcript variant 1, mGFP tagged 10 ug
$600.00
RC200457L3 Lenti ORF clone of Human CD82 molecule (CD82), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC200457L4 Lenti ORF clone of Human CD82 molecule (CD82), transcript variant 1, mGFP tagged 10 ug
$600.00
RG200457 CD82 (tGFP-tagged) - Human CD82 molecule (CD82), transcript variant 1 10 ug
$489.00 $500.00
SC107926 CD82 (untagged)-Human CD82 molecule (CD82), transcript variant 1 10 ug
$300.00
SC324395 CD82 (untagged)-Human CD82 molecule (CD82), transcript variant 1 10 ug
$300.00

Plasmid Map for RC200457

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200457
Circular map for RC200457

file-alt Citations

CD82 suppresses CD44 alternative splicing-dependent melanoma metastasis by mediating U2AF2 ubiquitination and degradation
Zhang, Feng, Liu et al
Oncogene (2016)
Cited by 45
AI Summary: OriGene products contributed to this study by providing complementary DNA clones for CD44s, CD44v8-10, hemagglutinin-tagged CD82, and GST-tagged U2AF2, which were used for transfection experiments to investigate the molecular mechanisms underlying melanoma metastasis.
Snippet: Complementary DNA transfection. Complementary DNA clones of CD44s, CD44v8–10, hemagglutinin-tagged CD82 and GST-tagged U2AF2 (Origene, Rockville, MD, USA) were inserted into the expression vector pcDNA3.1 (Invitrogen, Carlsbad, CA, USA). ... Cite SaveSaving
Showing 1-1 of 1 papers.
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