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PRAM1 (NM_032152) Human Untagged Clone

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SKU
SC106058
PRAM1 (untagged)-Human PML-RARA regulated adaptor molecule 1 (PRAM1)
  $920.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PRAM1
Synonyms PML-RAR; PRAM-1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_032152, the custom clone sequence may differ by one or more nucleotides


ATGGCCCATCACCTGCCTGCAGCCATGGAGAGCCATCAGGACTTCCGGAGCATCAAAGCAAAGTTCCAGG
CCTCTCAGCCGGAGCCCAGCGACCTGCCCAAAAAACCTCCGAAGCCTGAGTTTGGTAAACTGAAGAAGTT
CTCCCAGCCTGAGCTAAGCGAGCACCCCAAGAAGGCCCCGCTGCCTGAGTTTGGTGCAGTGTCCTTGAAG
CCCCCGCCGCCTGAGGTCACTGACCTCCCCAAGAAGCCCCCGCCGCCTGAGGTCACTGACCTCCCCAAGA
AGCCCCCGCCGCCTGAGGTCACTGACCTCCCCAAGAAGCCCCCGCCGCCTGAGGTCACTGACCTCCCCAA
GAAGCCGTCCAAACTGGAGTTGAGTGACCTCTCCAAGAAGTTCCCACAGCTGGGGGCCACTCCGTTTCCA
AGGAAGCCCCTGCAGCCTGAGGTCGGTGAGGCCCCTTTGAAGGCCTCGCTGCCGGAGCCTGGTGCGCCGG
CCCGGAAACCCCTGCAGCCCGACGAACTCAGTCACCCCGCCAGACCCCCCTCCGAACCCAAATCCGGCGC
ATTCCCCAGGAAGCTCTGGCAACCCGAGGCCGGTGAGGCTACCCCGAGGTCCCCGCAGCCTGAGTTGAGT
ACCTTTCCCAAGAAGCCTGCGCAGCCTGAGTTCAACGTGTACCCCAAAAAGCCTCCGCAGCCTCAGGTCG
GTGGCCTCCCTAAGAAGTCCGTGCCGCAGCCTGAGTTCAGCGAGGCCGCTCAGACTCCCCTCTGGAAGCC
TCAGTCCAGCGAGCCGAAGCGCGACTCCAGCGCCTTTCCCAAAAAGGCCTCCCAGCCTCCGCTGAGTGAC
TTTCCCAAGAAGCCTCCGCAGCCTGAGCTTGGGGACCTCACCAGGACCTCCTCAGAGCCCGAAGTCAGCG
TGCTTCCCAAGAGGCCGCGGCCGGCCGAATTCAAAGCGCTCTCCAAGAAGCCCCCGCAGCCCGAGCTGGG
CGGCCTCCCCAGGACCTCCTCAGAGCCCGAGTTCAACTCACTCCCCAGGAAGCTGCTGCAGCCGGAGCGC
CGGGGGCCACCCCGCAAGTTCTCACAGCCTGAGCCCAGCGCTGTCCTCAAGAGACACCCGCAGCCTGAGT
TCTTCGGTGATCTCCCTCGAAAGCCTCCACTCCCCAGCTCCGCTTCCGAGAGCTCACTGCCTGCGGCCGT
GGCCGGCTTCAGCTCCCGGCACCCGCTCAGCCCTGGGTTTGGAGCGGCTGGGACACCCCGCTGGAGGTCA
GGAGGCCTGGTTCACAGTGGAGGGGCCAGGCCAGGCCTCAGACCCAGCCATCCACCCCGGCGGAGGCCTC
TGCCCCCTGCCAGCAGCCTGGGACACCCTCCAGCCAAGCCCCCGCTGCCCCCGGGGCCCGTGGATATGCA
GAGCTTTCGGAGACCCTCTGCAGCATCCATAGATCTACGGAGGACCCGCTCGGCCGCTGGGCTCCACTTC
CAGGACCGACAGCCTGAAGACATCCCGCAGGTCCCAGATGAGATCTACGAGCTGTATGACGATGTGGAAC
CCAGAGATGACTCCAGCCCCAGCCCCAAGGGCAGAGATGAAGCGCCCTCAGTTCAGCAAGCCGCCAGGAG
GCCACCACAAGACCCAGCGCTCAGGAAGGAGAAGGATCCCCAGCCACAGCAGTTGCCACCCATGGACCCA
AAGTTGCTGAAGCAGCTGAGGAAGGCAGAGAAGGCCGAGAGGGAGTTCCGGAAGAAGTTCAAGTTTGAAG
GGGAGATCGTGGTTCACACGAAGATGATGATCGACCCCAACGCTAAGACACGTCGCGGGGGTGGCAAGCA
CCTCGGGATCCGGCGCGGGGAGATCCTGGAGGTGATCGAGTTCACCAGCAATGAGGAGATGCTGTGCCGG
GACCCCAAAGGCAAATATGGCTACGTGCCCAGAACAGCGCTCCTGCCCCTGGAGACGGAGGTGTACGATG
ATGTCGACTTCTGCGATCCCCTGGAAAACCAACCACTCCCCCTGGGACGGTAA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_032152 unedited
NNNTTGTCAGATTTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACCAGGCTCGG
GTCCCACTCATCCCGTGGCCCATCACCTGCCTGCAGCCATGGAGAGCCATCAGGACTTCC
GGAGCATCAAAGCAAAGTTCCAGGCCTCTCAGCCGGAGCCCAGCGACCTGCCCAAAAAAC
CTCCGAAGCCTGAGTTTGGTAAACTGAAGAAGTTCTCCCAGCCTGAGCTAAGCGAGCACC
CCAAGAAGGCCCCGCTGCCTGAGTTTGGTGCAGTGTCCTTGAAGCCCCCGCCGCCTGAGG
TCACTGACCTCCCCAAGAAGCCCCCGCCGCCTGAGGTCACTGACCTCCCCAAGAAGCCCC
CGCCGCCTGAGGTCACTGACCTCCCCAAGAAGCCCCCGCCGCCTGAGGTCACTGACCTCC
CCAAGAAGCCGTCCAAACTGGAGTTGAGTGACCTCTCCAAGAAGTTCCCACAGCTGGGGG
CCACTCCGTTTCCAAGGAAGCCCCTGCAGCCTGAGGTCGGTGAGGCCCCTTTGAAGGCCT
CGCTGCCGGAGCCTGGTGCGCCGGCCCCGGAAACCCCTGCAGCCCGACGAACTCAGTCAC
CCCGCCAGACCCCCCTCCGAACCCAAATCCGGCGCATTCCCCAGGAAGCTCTGGCAACCC
GAGGCCGGTGAGGCTACCCCGAGGTCCCCGCAGCCTGAGTTGAGTACCTTTCCCAAGAAG
CCTGCGCAGCCTGAGTTCAACGTGTACCCCATAAAGCCTCCGCAGCCTCAGGNTCGGTGG
CCTACCTAAAAAGTNCGTGCCGCAGCCTGAGTTCAGCGAGGCCGCTCAGACTCCCCTCTG
GAAAGCTCAGTCCAGCGAGCCCGAGCGCGACTCCAGCGCCTTTCCCAAAAGGGACTCCAG
NCTNCGCTGANTGAC
Restriction Sites NotI-NotI |
ACCN NM_032152
Insert Size 2320 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032152.3, NP_115528.3
RefSeq Size 2189 bp
RefSeq ORF 2010 bp
Locus ID 84106
UniProt ID Q96QH2
Cytogenetics 19p13.2
Protein Families Druggable Genome
Summary The protein encoded by this gene is similar to FYN binding protein (FYB/SLAP-130), an adaptor protein involved in T cell receptor mediated signaling. This gene is expressed and regulated during normal myelopoiesis. The expression of this gene is induced by retinoic acid and is inhibited by the expression of PML-RARalpha, a fusion protein of promyelocytic leukemia (PML) and the retinoic acid receptor-alpha (RARalpha). provided by RefSeq, Jul 2008
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"NM_032152" in other vectors (6)
SKU Description Size Price
RC206972 PRAM1 (Myc-DDK-tagged)-Human PML-RARA regulated adaptor molecule 1 (PRAM1) 10 ug
$920.00
RC206972L1 Lenti ORF clone of Human PML-RARA regulated adaptor molecule 1 (PRAM1), Myc-DDK-tagged 10 ug
$1,220.00
RC206972L2 Lenti ORF clone of Human PML-RARA regulated adaptor molecule 1 (PRAM1), mGFP tagged 10 ug
$1,220.00
RC206972L3 Lenti ORF clone of Human PML-RARA regulated adaptor molecule 1 (PRAM1), Myc-DDK-tagged 10 ug
$1,220.00
RC206972L4 Lenti ORF clone of Human PML-RARA regulated adaptor molecule 1 (PRAM1), mGFP tagged 10 ug
$1,220.00
RG206972 PRAM1 (tGFP-tagged) - Human PML-RARA regulated adaptor molecule 1 (PRAM1) 10 ug
$1,120.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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