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PRAM1 (NM_032152) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC206972L4
Lenti ORF clone of Human PML-RARA regulated adaptor molecule 1 (PRAM1), mGFP tagged
ORF Plasmid:
mGFP DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $1,220.00
3 Weeks*
Size
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PRAM1
Synonyms PML-RAR; PRAM-1
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC206972).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032152
ORF Size 2010 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032152.3
RefSeq Size 2235 bp
RefSeq ORF 2013 bp
Locus ID 84106
UniProt ID Q96QH2
Cytogenetics 19p13.2
Protein Families Druggable Genome
MW 74 kDa
Summary The protein encoded by this gene is similar to FYN binding protein (FYB/SLAP-130), an adaptor protein involved in T cell receptor mediated signaling. This gene is expressed and regulated during normal myelopoiesis. The expression of this gene is induced by retinoic acid and is inhibited by the expression of PML-RARalpha, a fusion protein of promyelocytic leukemia (PML) and the retinoic acid receptor-alpha (RARalpha). provided by RefSeq, Jul 2008
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Documents
Resources
"NM_032152" in other vectors (6)
SKU Description Size Price
RC206972 PRAM1 (Myc-DDK-tagged)-Human PML-RARA regulated adaptor molecule 1 (PRAM1) 10 ug
$920.00
RC206972L1 Lenti ORF clone of Human PML-RARA regulated adaptor molecule 1 (PRAM1), Myc-DDK-tagged 10 ug
$1,220.00
RC206972L2 Lenti ORF clone of Human PML-RARA regulated adaptor molecule 1 (PRAM1), mGFP tagged 10 ug
$1,220.00
RC206972L3 Lenti ORF clone of Human PML-RARA regulated adaptor molecule 1 (PRAM1), Myc-DDK-tagged 10 ug
$1,220.00
RG206972 PRAM1 (tGFP-tagged) - Human PML-RARA regulated adaptor molecule 1 (PRAM1) 10 ug
$1,120.00
SC106058 PRAM1 (untagged)-Human PML-RARA regulated adaptor molecule 1 (PRAM1) 10 ug
$920.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.