DCUN1D5 (NM_032299) Human Untagged Clone

SKU SC105502

DCUN1D5 (untagged)-Human DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae) (DCUN1D5)

Product Data

• Type: Human Untagged Clone
• Target Symbol: DCUN1D5
• Synonyms: SCCRO5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC105502 sequence for NM_032299 edited (data generated by NextGen Sequencing); ATGCCGGTGAAGAAGAAGAGAAAATCCCCTGGGGTGGCAGCAGCAGTAGCGGAAGACGGA; GGCCTCAAAAAGTGTAAAATCTCCAGCTATTGCAGATCCCAACCCCCTGCTAGACTAATA; AGTGGAGAGGAACATTTTTCAAGCAAGAAGTGCCTGGCTTGGTTTTATGAATATGCAGGT; CCTGATGAAGTTGTAGGGCCAGAAGGAATGGAAAAATTTTGTGAAGACATTGGTGTTGAA; CCTGAAAATATTATTATGTTAGTTTTAGCGTGGAAATTGGAGGCTGAAAGCATGGGATTT; TTTACCAAGGAAGAATGGTTAAAGGGAATGACTTCATTACAGTGTGACTGCACAGAAAAG; TTACAAAACAAATTTGACTTTTTGCGCTCACAGTTGAATGATATTTCGTCATTTAAGAAT; ATCTACAGATATGCCTTTGATTTTGCAAGGGATAAAGATCAGAGAAGCCTTGATATTGAT; ACTGCTAAATCTATGTTAGCTCTTCTGCTTGGGAGGACATGGCCACTGTTTTCAGTATTT; TACCAGTACCTGGAGCAATCAAAGTATCGTGTTATGAACAAAGATCAATGGTACAATGTA; TTAGAATTCAGCAGAACAGTCCATGCTGATCTTAGTAACTATGATGAAGATGGTGCTTGG; CCTGTTCTTCTTGATGAATTTGTTGAGTGGCAAAAAGTCCGTCAGACATCATAG; ; Clone variation with respect to NM_032299.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032299 unedited; GTTTTGTATACGATACTATAGGCGGCCGCGAATTCGGCACGAGGGCTGTGACTGGTGGAG; CTGCCGCGCTGTCCGCGTTATCTCCTCCCGGTGAGAACGAACCGCAGTGTCCACCGGCGA; GGAGCCAGCCCTGTCCCGGTCAGAGAAAGACGACGAGGATACCTGGGAGCGGGCGGCGGC; CGGGCTGGGCCGCGCCGGTGCGGGCTGGCGACTCTGCTCCTCCGCTTGCTGCTGTCTCTG; GGAACTGGGTGCCAGCGCTGAGGGGCTTCCAGCGGACAGGGACCCCCTTCCCCGGCTCCC; CTGCCCACCCTGCCGGGGAGGGCGGAAGATGCCGGTGAAGAAGAAGAGAAAATCCCCTGG; GGTGGCAGCAGCAGTAGCGGAAGACGGAGGCCTCAAAAAGTGTAAAATCTCCAGCTATTG; CAGATCCCAACCCCCTGCTAGACTAATAAGTGGAGAGGAACATTTTTCAAGCAAGAAGTG; CCTGGCTTGGTTTTATGAATATGCAGGTCCTGATGAAGTTGTAGGGCCAGAAGGAATGGA; AAAATTTTGTGAAGACATTGGTGTTGAACCTGAAAATATTATTATGTTAGTTTTAGCGTG; GAAATTGGAGGCTGAAAGCATGGGATTTTTTACCAAGGAAGAATGGTTAAAGGGAATGAC; TTCATTACAGTGTGACTGCACANAAAAGTTACAAAACAAATTTGACTTTTTGCGCTCACA; GTTGAATGATATTTCGTCATTTAAGAATATCTACAGATATGCCTTTGATTNTGCAGGGGA; TAAGATCAGAGAAGCCTTGATATTGATACTGCTAAATCTATGTTAGCTCTTCTGCTTTGG; GAGACATGGCCACTGTTTTCAGTATTNTACCAGTACCTGGNAGCATCAAGTATCGTGTTA; TGAACAAAGATCATGGGGTACATGTATTAGAATCAGCAGACAGTCCATGCTGATCTTAGT; ACTATGATGAGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032299 unedited; TGGACCGCGGCACGCATTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATTAAAATGCCCT; CAATTTATTTGATGCCTTAGACATTTAAAACATTAAGCTGTAAGAGCCTTGAAGGCCAGA; ACAAAGTTTTATCAGAGATGAATTATCACAATAGTTTTAAGTACACTTCTTTAAGAGGGC; AGAAGAACAACACCTGGCATTCTTACCAATTACACCTCAAACTGAACATATATTTCAATA; AATCCTTTCATAATTTCACATTAGCAAGTAGGCAGACAGGACATGTTTTGTTGAAGAAAT; ACTTCAAAGTGAAATGTTTCATTTTATACGTATGTATCATGTATTCTGAATCTGTGCTAT; TCCACATACCTTGTATGCCTTGAAAACTCTGGAGTTATCTCAGAATTTACTCCCACCCCG; ACCCCGAGTGATGGCATTCAATATTAAGATATAATTTTTGGTTTGCTAGATGAAAAGGCA; TTTGTATTTAAGAGAAATACTCCTCAATCTAACATTCCCAAAGAAGGCTTCAAATTGCAG; CTCAAGAAACACTAATCTAGAGTGCTTCTGAGTAAAAACCAATAAAATCTATACATTATA; CACAAAAAATGCTACTACTGGCAAAAATGTATTATATCAATAATTTTATCACCAGCTTTT; AAGAACTAAGAAATCCTTACACACTAGAAGACAAACCTGGTAATGCAGCCAGGGCCAGAC; CACAATCCCCCGTTTTCATTACACACCTGTCACCTGAATCCCTTGGCCATTTCCCTAAAG; GTTTTACCTCCTTACCTTATTAAAAATTATGGCCCCCAAGGACCGGGGGGGTGGGGGGGT; TGCATTTTGCCAAATCAAATTTGGGCCTTACTTCTCCCCAGAGGAATACCTTCGGTAAAT; CTCCTAAAAAACAACCCACCCCTGGTTAAAGCCCTTAGGGTTTCTTCCGGGGCAGGGGAG; GCCTCCGACATCTCCACGGGGCCGGGGAGGCCCTAACCCCCTCCAAAAGGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_032299
• Insert Size: 4420 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032299.2, NP_115675.1
• RefSeq Size: 1277 bp
• RefSeq ORF: 714 bp
• Locus ID: 84259
• UniProt ID: Q9BTE7
• Cytogenetics: 11q22.3
• Domains: DUF298
• Summary: Contributes to the neddylation of all cullins by transfering NEDD8 from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes which is necessary for the activation of cullin-RING E3 ubiquitin ligases (CRLs) (PubMed:26906416, PubMed:23201271, PubMed:19617556). May play a role in DNA damage response and may participate to cell proliferation and anchorage-independent cell growth (PubMed:23098533, PubMed:24192928).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.