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WNT6 (NM_006522) Human Untagged Clone

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SKU
SC103558
WNT6 (untagged)-Human wingless-type MMTV integration site family, member 6 (WNT6)
  $457.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol WNT6
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_006522 edited
GAATTCGGCACCAGGAGACACAGGCGCTGGCTGCCCCGTCCGCTCTCCGCCTCCGCCGCG
CCCTCCTCGCCCGGGATGGGCCCCCCCGCCGCCGCCGGATCCCTCGCCTCCCGGCCGCCG
CCGTTGCGCTCGCCGCGCTCGCACTGAAGCCCGGGCCCTCGCGCGCCGCGGTTCGCCCCG
CAGCCTCGCCCCCTGCCCACCCGGGCGGCCGTAGGGCGGTCACGATGCTGCCGCCCTTAC
CCTCCCGCCTCGGGCTGCTGCTGCTGCTGCTCCTGTGCCCGGCGCACGTCGGCGGACTGT
GGTGGGCTGTGGGCAGCCCCTTGGTTATGGACCCTACCAGCATCTGCAGGAAGGCACGGC
GGCTGGCCGGGCGGCAGGCCGAGTTGTGCCAGGCTGAGCCGGAAGTGGTGGCAGAGCTAG
CTCGGGGCGCCCGGCTCGGGGTGCGAGAGTGCCAGTTCCAGTTCCGCTTCCGCCGCTGGA
ATTGCTCCAGCCACAGCAAGGCCTTTGGACGCATCCTGCAACAGGACATTCGGGAGACGG
CCTTCGTGTTCGCCATCACTGCGGCCGGCGCCAGCCACGCCGTCACGCAGGCCTGTTCTA
TGGGCGAGCTGCTGCAGTGCGGCTGCCAGGCGCCCCGCGGGCGGGCCCCTCCCCGGCCCT
CCGGCCTGCCCGGCACCCCCGGACCCCCTGGCCCCGCGGGCTCCCCGGAAGGCAGCGCCG
CCTGGGAGTGGGGAGGCTGCGGCGACGACGTGGACTTCGGGGACGAGAAGTCGAGGCTCT
TTATGGACGCGCGGCACAAGCGGGGACGCGGAGACATCCGCGCGTTGGTGCAACTGCACA
ACAACGAGGCGGGCAGGCTGGCCGTGCGGAGCCACACGCGCACCGAGTGCAAATGCCACG
GGCTGTCGGGATCATGCGCGCTGCGCACCTGCTGGCAGAAGCTGCCTCCATTTCGCGAGG
TGGGCGCGCGGCTGCTGGAGCGCTTCCACGGCGCCTCACGCGTCATGGGCACCAACGACG
GCAAGGCCCTGCTGCCCGCCGTCCGCACGCTCAAGCCGCCGGGCCGAGCGGACCTCCTCT
ACGCCGCCGATTCGCCCGACTTCTGCGCCCCCAACCGACGCACCGGCTCCCCCGGCACGC
GCGGTCGCGCCTGCAATAGCAGCGCCCCGGACCTCAGCGGCTGCGACCTGCTGTGCTGCG
GCCGCGGGCACCGCCAGGAGAGCGTGCAGCTCGAAGAGAACTGCCTGTGCCGCTTCCACT
GGTGCTGCGTAGTACAGTGCCACCGCTGCCGTGTGCGCAAGGAGCTCAGCCTCTGCCTGT
GACCCGCCGCCCGGCCGCTAGACTGACTTCGCGCAGCGGTGGCTCGCACCTGTGGGACCT
CAGGGCACCGGCACCGGGCGCCTCTCGCCGCTCGAGCCCAGCCTCTCCCTGCCAAAGCCC
AACTCCCAGGGCTCTGGAAATGGTGAGGCGAGGGGCTTGAGAGGAACGCCCACCCACGAA
GGCCCAGGGCGCCAGACGGCCCCGAAAAGGCGCTCGGGGAGCGTTTAAAGGACACTGTAC
AGGCCCTCCCTCCCCTTGGCCTCTAGGAGGAAACAGTTTTTTAGACTGGAAAAAAGCCAG
TCTAAAGGCCTCTGGATACTGGGCTCCCCAGAACTGCTGGCCACAGGATGGTGGGTGAGG
TTAGTATCAATAAAGATATTTAAACCACCAAAAAAAAAAAAAAAAAAACTCGAC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006522 unedited
GTCAGAATTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACCAGAGACACAG
CGCTGGCTGCCCCGTCCGCTCTCCGCCTCCGCCGCGCCCTCCTCGCCCGGGATGGGCCCC
CCCGCCGCCGCCGGATCCCTCGCCTCCCGGCCGCCGCCGTTGCGCTCGCCGCGCTCGCAC
TGAAGCCCGGGCCCTCGCGCGCCGCGGTTCGCCCCGCAGCCTCGCCCCCTGCCCACCCGG
GCGGCCGTAGGGCGGTCACGATGCTGCCGCCCTTACCCTCCCGCCTCGGGCTGCTGCTGC
TGCTGCTCCTGTGCCCGGCGCACGTCGGCGGACTGTGGTGGGCTGTGGGCAGCCCCTTGG
TTATGGACCCTACCAGCATCTGCAGGAAGGCACGGCGGCTGGCCGGGCGGCAGGCCGAGT
TGTGCCAGGCTGAGCCGGAAGTGGTGGCAGAGCTAGCTCGGGGCGCCCGGCTCGGGGTGC
GAGAGTGCCAGTTCCAGTTCCGCTTCCGCCGCTGGAATTGCTCCAGCCACAGCAAGGCCT
TTGGACGCATCCTGCAACAGGACATTCGGGAGACGGCCTTCGTGTTCGCCATCACTGCGG
CCGGCGCCAGCCACGCCGTCACGCAGGCCTGTTCTATGGGCGAGCTGCTGCAGTGCGGCT
GCCAGGCGCCCCGCGGGCGGGCCCCTCCCCGGCCCTCCGGCCTGCCCGGCACCCCCGGAC
CCCCTGGCCCCGCGGGCTCCCCGGAAGGCAGCGCCGCCTGGGGAGTGGGGGAGGCTGCNG
CGACNACNTGGACTTCGGGGACGAGAAGTCGAGGCTCTTTATGGACGCGCGGCACAAGCG
GGGACGCGNAGACATNCGCGCGTTGGTGGCACTGCACNAACACGAGCGGNNCAGCCTGGC
CGTGCGAGCCCACGCGCACCGATGCAATGCCACGGCTGTCGGGATCAGCNCGCTGCGACT
GCTGCAGAGCTGCTCATTTCC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_006522 unedited
NGGGTAACACTATGNACCGCGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTGG
TGGTTTAAATATCTTTATTGATACTAACCTCACCCACCATCCTGTGGCCAGCAGTTCTGG
GGAGCCCAGTATCCAGAGGCCTTTAGACTGGCTTTTTTCCAGTCTAAAAAACTGTTTCCT
CCTAGAGGCCAAGGGGAGGGAGGGCCTGTACAGTGTCCTTTAAACGCTCCCCGAGCGCCT
TTTCGGGGCCGTCTGGCGCCCTGGGCCTTCGTGGGTGGGCGTTCCTCTCAAGCCCCTCGC
CTCACCATTTCCAGAGCCCTGGGAGTTGGGCTTTGGCAGGGAGAGGCTGGGCTCGAGCGG
CGAGAGGCGCCCGGTGCCGGTGCCCTGAGGTCCCACAGGTGCGAGCCACCGCTGCGCGAA
GTCAGTCTAGCGGCCGGGCGGCGGGTCACAGGCAGAGGCTGAGCTCCTTGCGCACACGGC
AGCGGTGGCACTGTACTACGCAGCACCAGTGGAAGCGGCACAAGCAGTTCTGTTCGAGCT
GCACGCTCTCCTGGCGGTGCCCGCGGCCGCAGCACAACAAGTCGCAGCCGCTGAAGTCCG
GGGCGCTGCTTTTGCAAGCGCGAACGCGCCTGCCGGGGGAACCAGTGCGTCGGGTGGGGG
GCGCAAAATTGGGCGAAATCGCGGCGAAAGGAAGTTCCCTTCGGCCGGCGGCTTAACCTT
CCGACCGCGGGCAACAAGGGCTTTGCCTCGTTGGGGCCCTGACCCCCGAGGCGCCTGGAA
ACGCTTCAAAAACCGGTCCCCCCTTTTGGAAAGGGAGCGATTTCTTGCACCTGTTCGCCC
GCGCAAGTTCCCCAAGGCGGGGCTATTGCCTCCGGGCGGGTGGGGTTCCCCCCGGC
Restriction Sites NotI-NotI |
ACCN NM_006522
Insert Size 1730 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006522.3, NP_006513.1
RefSeq Size 1700 bp
RefSeq ORF 1098 bp
Locus ID 7475
UniProt ID Q9Y6F9
Cytogenetics 2q35
Domains wnt
Protein Families Adult stem cells, Cancer stem cells, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway, Transmembrane
Protein Pathways Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway
Summary The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is overexpressed in cervical cancer cell line and strongly coexpressed with another family member, WNT10A, in colorectal cancer cell line. The gene overexpression may play key roles in carcinogenesis. This gene and the WNT10A gene are clustered in the chromosome 2q35 region. The protein encoded by this gene is 97% identical to the mouse Wnt6 protein at the amino acid level. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_006522" in other vectors (6)
SKU Description Size Price
RC202575 WNT6 (Myc-DDK-tagged)-Human wingless-type MMTV integration site family, member 6 (WNT6) 10 ug
$457.00
RC202575L1 Lenti ORF clone of Human wingless-type MMTV integration site family, member 6 (WNT6), Myc-DDK-tagged 10 ug
$757.00
RC202575L2 Lenti ORF clone of Human wingless-type MMTV integration site family, member 6 (WNT6), mGFP tagged 10 ug
$757.00
RC202575L3 Lenti ORF clone of Human wingless-type MMTV integration site family, member 6 (WNT6), Myc-DDK-tagged 10 ug
$757.00
RC202575L4 Lenti ORF clone of Human wingless-type MMTV integration site family, member 6 (WNT6), mGFP tagged 10 ug
$757.00
RG202575 WNT6 (tGFP-tagged) - Human wingless-type MMTV integration site family, member 6 (WNT6) 10 ug
$489.00 $657.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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