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EVER2 (TMC8) (NM_152468) Human Untagged Clone

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SKU
SC101329
TMC8 (untagged)-Human transmembrane channel-like 8 (TMC8)
  $1,890.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EVER2
Synonyms EV2; EVER2; EVIN2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_152468, the custom clone sequence may differ by one or more nucleotides


ATGCTGCTGCCGCGGTCGGTGTCATCGGAGCGGGCCCCTGGGGTGCCGGAGCCGGAGGAGCTGTGGGAGG
CAGAGATGGAGCGGCTGCGCGGCTCTGGGACGCCCGTGCGCGGGCTGCCCTATGCCATGATGGACAAGCG
CCTCATCTGGCAGCTGCGGGAGCCCGCGGGGGTGCAGACCTTGCGCTGGCAGCGGTGGCAGCGCCGGCGG
CAGACGGTGGAAAGGCGCCTGCGGGAGGCAGCGCAGCGGCTGGCCCGGGGCCTTGGGCTCTGGGAGGGGG
CGCTCTACGAGATCGGGGGCCTCTTCGGCACAGGAATTCGGTCCTACTTCACCTTCCTCCGCTTCCTGCT
GCTACTCAACCTGCTGAGCCTGCTGCTCACCGCAAGCTTCGTGCTGCTGCCCCTGGTCTGGCTCCGCCCC
CCTGACCCAGGCCCCACCCTGAACTTGACCCTCCAGTGCCCTGGTAGCCGCCAGTCCCCGCCTGGCGTTT
TGAGGTTCCACAATCAACTTTGGCATGTTTTGACTGGCAGGGCCTTCACCAACACCTATCTCTTCTACGG
TGCGTACCGAGTGGGGCCGGAGAGCAGCTCCGTGTACAGCATCCGCCTGGCCTACCTCCTCAGCCCGCTG
GCCTGCCTGCTCCTCTGCTTCTGTGGGACTCTGCGGCGGATGGTGAAGGGGCTGCCGCAGAAGACTCTGC
TGGGTCAGGGCTATCAGGCGCCTCTCAGCGCCAAGGTCTTCTCCTCATGGGACTTCTGCATCCGGGTGCA
GGAAGCAGCCACCATCAAGAAGCATGAGATCAGCAACGAGTTCAAGGTGGAGCTGGAGGAGGGCCGTCGC
TTCCAGCTGATGCAGCAGCAGACCCGGGCCCAGACGGCCTGCCGCCTGCTCTCCTACCTGCGGGTCAACG
TACTCAACGGGCTCCTGGTGGTTGGGGCCATCAGCGCCATCTTCTGGGCTACCAAGTACTCACAGGACAA
CAAGGAGGAGTCCCTGTTTCTGCTGCTCCAGTACCTGCCCCCTGGGGTCATCGCCCTGGTCAACTTCCTG
GGTCCCCTGCTGTTCACATTTCTGGTCCAGCTGGAGAACTACCCTCCCAACACGGAGGTCAACCTCACTC
TGATCTGGTGCGTGGTGCTGAAGCTGGCCAGCTTGGGGATGTTCTCCGTCTCCCTGGGTCAGACCATACT
GTGCATTGGCAGAGACAAGAGCAGCTGTGAGTCCTACGGCTACAACGTTTGTGACTATCAGTGCTGGGAG
AACTCCGTGGGGGAGGAGCTGTACAAGCTGAGTATCTTCAACTTCCTCCTCACCGTGGCCTTCGCCTTCC
TGGTCACCCTGCCTCGGAGGCTGCTGGTGGACCGGTTCTCAGGCCGGTTCTGGGCCTGGCTGGAACGGGA
GGAGTTCCTGGTCCCCAAGAATGTGCTGGACATCGTGGCGGGGCAGACGGTCACCTGGATGGGCCTCTTC
TACTGCCCCCTGCTGCCCCTGCTGAATAGCGTCTTCCTCTTCCTCACCTTCTACATCAAGAAGTACACCC
TCCTGAAGAACTCCAGGGCATCTTCGCGGCCCTTCCGTGCCTCCAGCTCCACCTTCTTCTTCCAGCTAGT
GCTCCTCCTGGGCCTGCTTCTGGCTGCAGTGCCCCTGGGCTATGTGGTCAGCAGCATCCACTCCTCCTGG
GACTGCGGCCTCTTCACCAACTACTCAGCACCCTGGCAAGTGGTCCCGGAGCTGGTGGCCCTTGGGCTCC
CGCCCATTGGCCAGCGTGCCCTCCACTACCTGGGCTCCCACGCCTTCAGCTTCCCCCTCCTCATCATGCT
CAGCCTTGTCCTGACGGTGTGCGTCTCCCAGACCCAGGCCAATGCCAGGGCCATCCACAGGCTCCGGAAG
CAGCTGGTGTGGCAGGTTCAGGAGAAGTGGCACCTGGTGGAGGACCTGTCGCGACTGCTGCCGGAGCCAG
GCCCGAGCGACTCTCCGGGCCCCAAGTACCCTGCCTCCCAAGCTTCGCGCCCGCAGTCCTTCTGCCCCGG
ATGCCCATGCCCTGGCTCCCCGGGCCACCAGGCCCCGCGGCCGGGCCCCTCCGTCGTGGATGCCGCGGGA
CTGCGTTCCCCTTGCCCTGGACAGCACGGTGCCCCGGCCTCCGCCCGCAGATTCCGCTTCCCCAGCGGCG
CGGAGCTGTAA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_152468 unedited
AGTATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCCAGGNACTT
GGTCCCTAGGTCCCCAGATGGGGAGACTGAGGCCGTGGCTGTGTGTCCCTCTGAGAGTTG
GAGCGGGGCTGGGCCCGAATTCGACCCCAGCAGGATTCTCTCTCATTTCTGAGCCCCGGA
GGTGGCAGAGCGGCAGACCCGGGCAAGTGAACCCTAGGGCTGCAGGAGCCCAGGCCCCGA
CGCCGGCGCAGAGGGGACGGAAGGGCCCGCCCCCAGCCCAGCGTGCACAGAGGCCATAGC
CAAGGCCTTAAGGCTCATCCAACCGGGGACTCATATCCCCCCCACCGGCAGCCCGGCGCC
CCAGCCTCTACCCGTGCCCGCCGAGATGCTGCTGCCGCGGTCGGTGTCATCGGAGCGGGC
CCCTGGGGTGCCGGAGCCGGAGGAGCTGTGGGAGGCAGAGATGGAGCGGCTGCGCGGCTC
TGGGACGCCCGTGCGCGGGCTGCCCTATGCCATGATGGACAAGCGCCTCATCTGGCCTCT
TCGGCACAGGAATTCGGTCCTACTTCACCTTCCTCCGCTTTCTGCTGCTACTCAACCTGC
TGAGCCTGCTGCTCACCGCAAGCTTCGTGCTGCTGCCCCTGGGCTGGCTCCGGCCCCCTG
ACCCAGCCCCACCCTGAACTTGACCCTTCAGTGCCCTGGTAGCCGNCACGTCCCGCCTGG
CGTTTTGAAGTTCCACACTCAACTCTGGCATGTTTTGACTGTCAGGGCCTTTACCACCAC
TAACTCTTCTACCGTGCGTACCGAGTGGGCCCGGAAAGCANTTCGTGTACAGCATTCGGC
TGGGCTACTCCCTAGACCGCTGGCTGCCTGCTCTTTGTTTTGTGGACTCTCCGCCGAGGT
GAAGGGCTGCCCCAAAAACTTGTTTTTCAGGGTTTAAGCGCCTTATGCCCAGTGTTCCTC
ATGGACTT
Restriction Sites NotI-NotI |
ACCN NM_152468
Insert Size 2500 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152468.3, NP_689681.2
RefSeq Size 4419 bp
RefSeq ORF 4419 bp
Locus ID 147138
UniProt ID Q8IU68
Cytogenetics 17q25.3
Protein Families Transmembrane
Summary Epidermodysplasia verruciformis (EV) is an autosomal recessive dermatosis characterized by abnormal susceptibility to human papillomaviruses (HPVs) and a high rate of progression to squamous cell carcinoma on sun-exposed skin. EV is caused by mutations in either of two adjacent genes located on chromosome 17q25.3. Both of these genes encode integral membrane proteins that localize to the endoplasmic reticulum and are predicted to form transmembrane channels. This gene encodes a transmembrane channel-like protein with 8 predicted transmembrane domains and 3 leucine zipper motifs. provided by RefSeq, Jul 2008
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"NM_152468" in other vectors (6)
SKU Description Size Price
RC210309 TMC8 (Myc-DDK-tagged)-Human transmembrane channel-like 8 (TMC8) 10 ug
$997.00
RC210309L1 Lenti-ORF clone of TMC8 (Myc-DDK-tagged)-Human transmembrane channel-like 8 (TMC8) 10 ug
$1,297.00
RC210309L2 Lenti-ORF clone of TMC8 (mGFP-tagged)-Human transmembrane channel-like 8 (TMC8) 10 ug
$1,297.00
RC210309L3 Lenti-ORF clone of TMC8 (Myc-DDK-tagged)-Human transmembrane channel-like 8 (TMC8) 10 ug
$1,297.00
RC210309L4 Lenti-ORF clone of TMC8 (mGFP-tagged)-Human transmembrane channel-like 8 (TMC8) 10 ug
$1,297.00
RG210309 TMC8 (tGFP-tagged) - Human transmembrane channel-like 8 (TMC8) 10 ug
$1,197.00

file-alt Citations

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