CCDC50 (NM_174908) Human Untagged Clone

SKU
SC101288
CCDC50 (untagged)-Human coiled-coil domain containing 50 (CCDC50), transcript variant 1
$300.00
In Stock*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CCDC50
Synonyms C3orf6; DFNA44; YMER
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC101288 sequence for NM_174908 edited (data generated by NextGen Sequencing)
ATGGCTGAAGTCAGCATCGACCAGTCCAAGCTGCCTGGAGTCAAGGAAGTATGCCGAGAT
TTTGCTGTCCTGGAGGACCACACCCTGGCTCACAGCCTGCAGGAACAAGAGATTGAGCAT
CATTTGGCATCGAACGTTCAGCGGAACCGTTTGGTCCAGCATGATCTCCAGGTGGCTAAG
CAGCTCCAAGAGGAAGATCTGAAAGCGCAGGCCCAGCTCCAGAAGCGCTACAAAGACCTT
GAACAACAAGACTGTGAAATTGCTCAGGAAATTCAGGAGAAGCTGGCTATTGAGGCAGAG
AGACGACGCATTCAGGAGAAGAAGGATGAGGACATAGCTCGCCTTTTGCAAGAAAAGGAG
TTACAGGAAGAGAAAAAGAGAAAGAAACACTTTCCAGAGTTCCCTGCAACCCGTGCTTAT
GCAGATAGTTACTATTATGAAGATGGAGGAATGAAGCCAAGAGTGATGAAAGAAGCTGTA
TCTACTCCATCACGAATGGCCCACAGGGATCAGGAATGGTATGATGCTGAAATTGCCAGA
AAACTGCAAGAAGAAGAACTTTTGGCTACCCAGGTGGACATGAGAGCCGCTCAAGTAGCT
CAAGATGAAGAAATCGCTCGACTTCTAATGGCTGAAGAAAAGAAAGCTTACAAAAAAGCC
AAGGAGCGGGAGAAATCATCTTTGGACAAAAGAAAGCAAGACCCCGAGTGGAAGCCAAAA
ACAGCTAAAGCAGCAAATTCCAAGTCAAAAGAGAGTGATGAACCTCACCATTCTAAGAAT
GAAAGGCCAGCACGGCCACCACCACCTATCATGACAGATGGTGAAGATGCGGATTACACT
CATTTTACAAACCAGCAGAGTTCCACACGGCATTTCTCAAAATCAGAGTCCTCTCATAAA
GGTTTTCATTACAAACATTAA

Clone variation with respect to NM_174908.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_174908 unedited
GCGGCCGCGAATTCGGCACCAGGATATTTGGTATCGATTGGGGCCGGGGACGCGGAGCAG
GTGGCCGCGGCGGGGCAGCTGGGCCGCCAGCTTGGTGCCTCGGGGACCGTCTCCCGCTGC
TTTGGTCACCAGCCCCTGCCCGCCCGACCCGCTCCGTTCTCCGGCCTGCGAGCCCTGCCG
GCCGGACTTTGCGCCGCGTCCGGCGCTGCTGCTGCGCTCGGGGCCCCGCTCGGCGCCGGC
GGTGACCGGGAAGCCCGCGTTAAAGGGGCAACCGGGACCCTGGCCCGGTATGGCTGAAGT
CAGCATCGACCAGTCCAAGCTGCCTGGAGTCAAGGAAGTATGCCGAGATTTTGCTGTCCT
GGAGGACCACACCCTGGCTCACAGCCTGCAGGAACAAGAGATTGAGCATCATTTGGCATC
GAACGTTCAGCGGAACCGTTTGGTCCAGCATGATCTCCAGGTGGCTAAGCAGCTCCAAGA
GGAAGATCTGAAAGCGCAGGCCCAGCTCCAGAAGCGCTACAAAGACCTTGAACAACAAGA
CTGTGAAATTGCTCAGGAAATTCAGGAGAAGCTGGCTATTGAGGCAGAGAGACGACGCAT
TCAGGAGAAGAAGGATGAGGACATANCTCGGCTTTTGCAGANAAGGAGTTACAGGGAGAG
AAAAAGAGAAAGAAACACTTTCCAGAGTTCCCTGCACCCGTGCTTATGCAGATAGTTACT
ATTATGAAGATGGG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_174908 unedited
TCGACCATTGGAGAGGCAACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGGCA
GCCACCCGGGATCTGTTCAGGAAAAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTT
TTTTTTTTTTTTTTTTTGCTTTCACATTTATATAAAAATGACAAAGTAAAAAGCAGAGAA
CACAGTGAAAAGTGTCTGGCAGTTCATTAAAATACAGTTGAGTTGCTTCTATAGTCTCAA
ACATTATTATATTATTTGAATGAGAAAGAGTATGAGGATTTAACTGGCTGAATTTCATTT
CTATCTCCTTATTCATAGGGTAATAATTACTCTGATATTATTTAAAAAGTGTTTGCTTTA
CACAAAAATAAAATAAACCTAAAATATTTTAAAATTGTAATCTTTTAAAAACCTTTAAAA
AAAGTAACAAATTTTCAATAAAGATGTTGAATTAGCTATCACAAGGTGATAGCTTAATTG
GTCACCTACTAAAACACCCGAAACACTAACATTCCCGGTAAGGGTTAAGTGGGCCCACTT
TTTTTGAAGGCATATCCCCGTTAAAATTTTCAAAAAAAAAAACTGGGGGAANTACTGGGA
AGCCGAAGAATGCCNAATTTTTTAAGGAATGGGTCCTGGGTAAAAAAAAAAAAAATATTT
TTGAAACAAGAAGGGGGAAGGCTGTGCCGCATTANNAAAAAAAGAGGGGTGTGTGTGCCN
NAANGNNNCNNAGAAAGCAGTGGGGGAGCGAGGNGAANNCNNGAGNGNAGCGGNNAGNNA
GCGCTAANACGGAGCAACGGGCANCGCNNNCNGATNNANAACGAGGGNGGGAGGGGGANC
GAGNNNGCCNNCANAGGGGNNNAANNNNAGNCAANNCNNCT
Restriction Sites NotI-NotI
ACCN NM_174908
Insert Size 4000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_174908.2, NP_777568.1
RefSeq Size 1926 bp
RefSeq ORF 921 bp
Locus ID 152137
UniProt ID Q8IVM0
Cytogenetics 3q28
Summary This gene encodes a soluble, cytoplasmic, tyrosine-phosphorylated protein with multiple ubiquitin-interacting domains. Mutations in this gene cause nonsyndromic, postlingual, progressive sensorineural DFNA44 hearing loss. In mouse, the protein is expressed in the inner ear during development and postnatal maturation and associates with microtubule-based structures. This protein may also function as a negative regulator of NF-kB signaling and as an effector of epidermal growth factor (EGF)-mediated cell signaling. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Transcript Variant: This variant (1) lacks an in-frame exon in the coding region, compared to variant 2, and encodes the short isoform. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.
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Citations

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