OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Pfkfb3 (NM_057135) Rat Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
RR209579
Pfkfb3 (Myc-DDK-tagged ORF) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), (10 ug)
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $568.00
5 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00

Other products for "Pfkfb3 or NM_057135"

Specifications
Specifications
Product Data
Type Rat Tagged ORF Clone
Target Symbol Pfkfb3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RR209579 representing NM_057135
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTTTGGAACTGACCCAGAGCCGGGTGCAGAAGATCTGGGTGCCTGTCGATCACCGACCCTCGTTGC
CCAGATCCTGTGGGCCGAAGCTGACCAACTCCCCAACGGTGATTGTGATGGTGGGGCTCCCAGCCCGGGG
TAAGACTTACATCTCCAAGAAGCTGACTCGCTACCTCAACTGGATAGGTGTTCCAACGAAAGTGTTCAAT
GTGGGAGAATACCGACGGGAGGCTGTGAAGCAATACAGCTCTTACAACTTCTTCCGCCCTGACAATGAGG
AGGCCATGAGAGTCCGAAAACAGTGTGCCTTAGCTGCCCTGAGAGACGTCAAAAGCTACCTGACAAAGGA
AGGAGGACAGATTGCAGTTTTTGATGCCACCAATACGACGAGAGAAAGGAGGCACATGATCCTTCATTTT
GCCAAAGAAAATGACTTCAAGGCATTTTTCATCGAGTCCGTCTGTGACGACCCTACAGTTGTGGCCTCCA
ATATCATGGAAGTGAAAATCTCCAGCCCGGATTACAAAGACTGCAACTCCGCAGAAGCCATGGACGACTT
CATGAAGAGGATCAACTGCTACGAAGCCAGCTACCAGCCTCTTGACCCTGATAAATGTGACAGGGACCTG
TCATTCATCAAAGTGATTGACGTTGGCCGGAGGTTCTTGGTGAACAGAGTGCAGGACCACATCCAGAGCC
GAATCGTGTATTACCTGATGAACATCCACGTGCAGCCCCGGACCATCTACCTGTGTCGGCACGGCGAGAA
TGAGTACAACGTGCAGGGCAAGATTGGGGGCGACTCAGGCCTGTCCAGCAGAGGCAAGAAGTTCGCCAAT
GCCCTGAGCAAGTTCGTTGAAGAGCAGAATCTGAAGGACCTCCGCGTGTGGACCAGTCAGCTGAAAAGTA
CCATCCAGACCGCGGAGGCCCTAAGGCTCCCCTACGAGCAGTGGAAGGCGCTCAATGAGATTGATGCTGG
TGTGTGTGAGGAACTAACCTATGAGGAGATCAGAGACACCTACCCTGAGGAGTATGCACTGCGTGAGCAA
GACAAATACTACTACCGCTATCCCACAGGCGAGTCCTACCAGGATCTTGTCCAGCGCCTGGAGCCTGTGA
TCATGGAATTAGAGCGCCAAGAGAACGTATTGGTCATCTGTCACCAGGCTGTCCTGCGCTGCCTACTAGC
CTACTTCCTTGACAAGAGTGCAGAGGAGATGCCATACCTGAAATGCCCACTTCACACAGTCCTGAAACTG
ACGCCCGTGGCGTATGGGTGCCGTGTGGAGTCCATTTACTTGAATGTAGAATCAGTGAGCACGCATCGGG
AGAGGTCAGAGGCTGTCAAAATACAACACTTTGCCAGTGTAGTCAGACCTTCCTCTTACACCGAACTTGA
CTTTCTGTCTGTGGAGTCCGCAAAACAGGATGCAAAGAAGGGACCTAACCCTCTCATGAGACGCAATAGT
GTCACCCCACTAGCCAGCCCCGAGCCCACCAAAAAGCCTCGCATCAACAGCTTTGAGGAGCATGTGGCTT
CCACTTCTGCTGCCCTGCCAAGCTGCCTGCCCCCGGAAGTGCCCACGCAGCTGCCCGGACAACCTTTGCT
AGGGAAAGCCTGTCTACGAACTGTCTGTCACATTTTTTCAAAGTTTTCTCCTTAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RR209579 representing NM_057135
Red=Cloning site Green=Tags(s)

MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFN
VGEYRREAVKQYSSYNFFRPDNEEAMRVRKQCALAALRDVKSYLTKEGGQIAVFDATNTTRERRHMILHF
AKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRINCYEASYQPLDPDKCDRDL
SFIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEYNVQGKIGGDSGLSSRGKKFAN
ALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQ
DKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKL
TPVAYGCRVESIYLNVESVSTHRERSEAVKIQHFASVVRPSSYTELDFLSVESAKQDAKKGPNPLMRRNS
VTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQPLLGKACLRTVCHIFSKFSPY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_057135
ORF Size 1665 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_057135.1, NP_476476.1
RefSeq Size 2148 bp
RefSeq ORF 1668 bp
Locus ID 117276
UniProt ID O35552
Cytogenetics 17q12.2
MW 63.7 kDa
Summary catalyzes the biosynthesis and degradation of fructose 2,6-bisphosphate, which plays a role in glycolysis RGD, Feb 2006
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_057135" in other vectors (3)
SKU Description Size Price
RN209579 Pfkfb3 (untagged ORF) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), (10 ug) 10 ug
$569.00
RR209579L3 Lenti ORF clone of Pfkfb3 (Myc-DDK-tagged ORF) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), (10 ug) 10 ug
$868.00
RR209579L4 Lenti ORF clone of Pfkfb3 (mGFP-tagged ORF) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), (10 ug) 10 ug
$868.00

Plasmid Map for RR209579

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RR209579
Circular map for RR209579

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products