RPH3AL (NM_001190413) Human Tagged ORF Clone

  • TrueORF®
SKU
RG230793
RPH3AL (tGFP-tagged) - Human rabphilin 3A-like (without C2 domains) (RPH3AL), transcript variant 4
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RPH3AL
Synonyms NOC2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG230793 representing NM_001190413
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGACACCATCTTCGGCAGCGGGAATGATCAGTGGGTTTGCCCCAATGACCGGCAGCTTGCCCTTC
GAGCCAAGCTGCAGACGGGCTGGTCCGTGCACACCTACCAGACGGAGAAGCAGAGGAGGAAGCAGCACCT
CAGCCCGGCGGAGGTGGAGGCCATCCTGCAGGTCATCCAGAGGGCAGAGCGGCTCGACGTCCTGGAGCAG
CAGAGAATCGGGCGGCTGGTGGAGCGGCTGGAGACCATGAGGCAGAATGTGATGGGGAACGGCCTGTCCC
AGTGTCTGCTCTGCGGGGAGGTGCTGGGCTTCCTGGGCAGCTCGTCGGTGTTCTGCAAAGACTGCAGGAA
GGTCTGGAAGAGGTCGGGGGCCTGGTTCTACAAAGGGCTCCCCAAGTATATCTTGCCCCTGAAGACCCCT
GGCCGAGCTGATGACCCCCACTTCCGACCTTTGCCCACGGAACCGGCAGAGCGAGAGCCCAGAAGCTCTG
AGACCAGCCGCATCTACACGTGGGCCCGAGGAAGAGTGGTTTCCAGTGACAGTGACAGTGACTCGGATCT
TAGCTCCTCCAGCCTAGAGGACAGACTCCCATCCACTGGGGTCAGGGACCGGAAAGGCGACAAACCCTGG
AAGGAGTCAGGTGGCAGCGTGGAGGCCCCCAGGATGGGGTTCACCCACCCGCCGGGCCACCTCTCTGGGT
GCCAGAGCAGCCTGGCCAGTGGTGAGACGGGGACAGGCTCTGCTGACCCGCCAGGGGGACCCCGCCCCGG
GCTGACCCGAAGGGCCCCGGTAAAAGACACACCTGGACGAGCCCCCGCTGCTGACGCAGCTCCAGCAGGC
CCCTCCAGCTGCCTGGGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG230793 representing NM_001190413
Red=Cloning site Green=Tags(s)

MADTIFGSGNDQWVCPNDRQLALRAKLQTGWSVHTYQTEKQRRKQHLSPAEVEAILQVIQRAERLDVLEQ
QRIGRLVERLETMRQNVMGNGLSQCLLCGEVLGFLGSSSVFCKDCRKVWKRSGAWFYKGLPKYILPLKTP
GRADDPHFRPLPTEPAEREPRSSETSRIYTWARGRVVSSDSDSDSDLSSSSLEDRLPSTGVRDRKGDKPW
KESGGSVEAPRMGFTHPPGHLSGCQSSLASGETGTGSADPPGGPRPGLTRRAPVKDTPGRAPAADAAPAG
PSSCLG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001190413
ORF Size 858 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001190413.1, NP_001177342.1
RefSeq Size 2491 bp
RefSeq ORF 861 bp
Locus ID 9501
UniProt ID Q9UNE2
Cytogenetics 17p13.3
Protein Families Secreted Protein
Summary The protein encoded by this gene plays a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells and plays a key role in insulin secretion by pancreatic cells. This gene is likely a tumor suppressor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Jun 2010
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Documents
Resources
"NM_001190413" in other vectors (5)
SKU Description Size Price
RC230793 RPH3AL (Myc-DDK-tagged)-Human rabphilin 3A-like (without C2 domains) (RPH3AL), transcript variant 4 10 ug
$300.00
RC230793L1 Lenti ORF clone of Human rabphilin 3A-like (without C2 domains) (RPH3AL), transcript variant 4, Myc-DDK-tagged 10 ug
$600.00
RC230793L2 Lenti ORF clone of Human rabphilin 3A-like (without C2 domains) (RPH3AL), transcript variant 4, mGFP tagged 10 ug
$600.00
RC230793L3 Lenti ORF clone of Human rabphilin 3A-like (without C2 domains) (RPH3AL), transcript variant 4, Myc-DDK-tagged 10 ug
$600.00
RC230793L4 Lenti ORF clone of Human rabphilin 3A-like (without C2 domains) (RPH3AL), transcript variant 4, mGFP tagged 10 ug
$600.00

Plasmid Map for RG230793

Download sequence for

Cloning scheme for RG230793

Circular map for RG230793

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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