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CUG BP1 (CELF1) (NM_001172639) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG230208
CELF1 (tGFP-tagged) - Human CUGBP, Elav-like family member 1 (CELF1), transcript variant 4
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $677.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CUG BP1
Synonyms BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP; hNab50; NAB50; NAPOR
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG230208 representing NM_001172639
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCGTTTAAGTTGGATTTCCTTCCAGAAATGATGGTGGATCATTGCTCTTTGAATTCCAGTCCCG
TCTCAAAGAAAATGAACGGCACCCTGGACCACCCAGACCAACCAGATCTTGATGCTATCAAGATGTTTGT
GGGCCAGGTTCCAAGGACCTGGTCTGAAAAGGACTTGCGGGAACTCTTCGAACAGTATGGTGCTGTGTAT
GAAATCAACGTCCTAAGGGATAGGAGCCAAAACCCGCCTCAGAGCAAAGGGTGCTGTTTTGTTACATTTT
ACACCCGTAAAGCTGCATTAGAAGCTCAGAATGCTCTTCACAACATGAAAGTCCTCCCAGGGATGCATCA
CCCTATACAGATGAAACCTGCTGACAGTGAGAAGAACAATGTGGAAGACAGGAAGCTGTTTATTGGTATG
ATTTCCAAGAAGTGCACTGAAAATGACATCCGAGTCATGTTCTCTTCGTTTGGACAGATTGAAGAATGCC
GGATATTGCGGGGACCTGATGGCCTGAGCCGAGGTTGTGCATTTGTGACTTTTACAACAAGAGCCATGGC
ACAGACGGCTATCAAGGCAATGCACCAAGCACAGACCATGGAGGGTTGCTCATCACCCATGGTGGTAAAA
TTTGCTGATACACAGAAGGACAAAGAACAGAAGAGAATGGCCCAGCAGCTCCAGCAGCAGATGCAGCAAA
TCAGCGCAGCATCTGTGTGGGGAAACCTTGCTGGTCTAAATACTCTTGGACCCCAGTATTTAGCACTTTA
TTTGCAGCTCCTTCAGCAGACTGCCTCCTCTGGGAACCTCAACACCCTGAGCAGCCTCCACCCAATGGGA
GGGTTGAATGCAATGCAGTTACAGAATTTGGCTGCACTAGCTGCTGCAGCTAGTGCAGCTCAGAACACAC
CAAGTGGTACCAATGCTCTCACTACATCCAGCAGTCCCCTCAGCGTGCTCACTAGTTCAGGGTCCTCACC
TAGCTCTAGCAGCAGTAATTCTGTCAACCCCATAGCCTCACTTGGAGCCCTGCAGACATTAGCTGGAGCA
ACGGCTGGCCTCAATGTTGGCTCTTTGGCAGGAATGGCTGCTTTAAATGGTGGCCTGGGCAGCAGTGGCC
TTTCCAATGGCACCGGGAGCACCATGGAGGCCCTCACTCAGGCCTACTCGGGTATCCAGCAATATGCTGC
TGCTGCGCTCCCCACTCTGTACAACCAGAATCTTCTGACACAGCAGAGTATTGGTGCTGCTGGAAGCCAG
AAGGAAGGTCCAGAGGGAGCCAACCTGTTCATCTACCACCTGCCCCAGGAGTTTGGTGATCAGGACCTGC
TGCAGATGTTTATGCCCTTTGGGAATGTCGTGTCTGCCAAGGTTTTCATAGACAAGCAGACAAACCTGAG
CAAGTGTTTTGGTTTTGTAAGTTACGACAATCCTGTTTCGGCCCAAGCTGCCATCCAGTCCATGAACGGC
TTTCAGATTGGCATGAAGCGGCTTAAAGTGCAGCTCAAACGTTCGAAGAATGACAGCAAGCCCTAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG230208 representing NM_001172639
Red=Cloning site Green=Tags(s)

MAAFKLDFLPEMMVDHCSLNSSPVSKKMNGTLDHPDQPDLDAIKMFVGQVPRTWSEKDLRELFEQYGAVY
EINVLRDRSQNPPQSKGCCFVTFYTRKAALEAQNALHNMKVLPGMHHPIQMKPADSEKNNVEDRKLFIGM
ISKKCTENDIRVMFSSFGQIEECRILRGPDGLSRGCAFVTFTTRAMAQTAIKAMHQAQTMEGCSSPMVVK
FADTQKDKEQKRMAQQLQQQMQQISAASVWGNLAGLNTLGPQYLALYLQLLQQTASSGNLNTLSSLHPMG
GLNAMQLQNLAALAAAASAAQNTPSGTNALTTSSSPLSVLTSSGSSPSSSSSNSVNPIASLGALQTLAGA
TAGLNVGSLAGMAALNGGLGSSGLSNGTGSTMEALTQAYSGIQQYAAAALPTLYNQNLLTQQSIGAAGSQ
KEGPEGANLFIYHLPQEFGDQDLLQMFMPFGNVVSAKVFIDKQTNLSKCFGFVSYDNPVSAQAAIQSMNG
FQIGMKRLKVQLKRSKNDSKPY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001172639
ORF Size 1536 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001172639.2
RefSeq Size 7434 bp
RefSeq ORF 1539 bp
Locus ID 10658
UniProt ID Q92879
Cytogenetics 11p11.2
Protein Families Druggable Genome
Summary Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001172639" in other vectors (4)
SKU Description Size Price
RC230208 CELF1 (Myc-DDK-tagged)-Human CUGBP, Elav-like family member 1 (CELF1), transcript variant 4 10 ug
$477.00
RC230208L3 Lenti-ORF clone of CELF1 (Myc-DDK-tagged)-Human CUGBP, Elav-like family member 1 (CELF1), transcript variant 4 10 ug
$777.00
RC230208L4 Lenti-ORF clone of CELF1 (mGFP-tagged)-Human CUGBP, Elav-like family member 1 (CELF1), transcript variant 4 10 ug
$777.00
SC328846 CELF1 (untagged)-Human CUGBP Elav-like family member 1 (CELF1) transcript variant 4 10 ug
$525.00

Plasmid Map for RG230208

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for RG230208
Circular map for RG230208

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