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Hyaluronidase PH20 (SPAM1) (NM_001174045) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG230203
SPAM1 (tGFP-tagged) - Human sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant 4
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $674.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Hyaluronidase PH20 or NM_001174045"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Hyaluronidase PH20
Synonyms HEL-S-96n; HYA1; HYAL1; HYAL3; HYAL5; PH-20; PH20; SPAG15
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG230203 representing NM_001174045
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGTGCTAAAATTCAAGCACATCTTTTTCAGAAGCTTTGTTAAATCAAGTGGAGTATCCCAGATAG
TTTTCACCTTCCTTCTGATTCCATGTTGCTTGACTCTGAATTTCAGAGCACCTCCTGTTATTCCAAATGT
GCCTTTCCTCTGGGCCTGGAATGCCCCAAGTGAATTTTGTCTTGGAAAATTTGATGAGCCACTAGATATG
AGCCTCTTCTCTTTCATAGGAAGCCCCCGAATAAACGCCACCGGGCAAGGTGTTACAATATTTTATGTTG
ATAGACTTGGCTACTATCCTTACATAGATTCAATCACAGGAGTAACTGTGAATGGAGGAATCCCCCAGAA
GATTTCCTTACAAGACCATCTGGACAAAGCTAAGAAAGACATTACATTTTATATGCCAGTAGACAATTTG
GGAATGGCTGTTATTGACTGGGAAGAATGGAGACCCACTTGGGCAAGAAACTGGAAACCTAAAGATGTTT
ACAAGAATAGGTCTATTGAATTGGTTCAGCAACAAAATGTACAACTTAGTCTCACAGAGGCCACTGAGAA
AGCAAAACAAGAATTTGAAAAGGCAGGGAAGGATTTCCTGGTAGAGACTATAAAATTGGGAAAATTACTT
CGGCCAAATCACTTGTGGGGTTATTATCTTTTTCCGGATTGTTACAACCATCACTATAAGAAACCCGGTT
ACAATGGAAGTTGCTTCAATGTAGAAATAAAAAGAAATGATGATCTCAGCTGGTTGTGGAATGAAAGCAC
TGCTCTTTACCCATCCATTTATTTGAACACTCAGCAGTCTCCTGTAGCTGCTACACTCTATGTGCGCAAT
CGAGTTCGGGAAGCCATCAGAGTTTCCAAAATACCTGATGCAAAAAGTCCACTTCCGGTTTTTGCATATA
CCCGCATAGTTTTTACTGATCAAGTTTTGAAATTCCTTTCTCAAGATGAACTTGTGTATACATTTGGCGA
AACTGTTGCTCTGGGTGCTTCTGGAATTGTAATATGGGGAACCCTCAGTATAATGCGAAGTATGAAATCT
TGCTTGCTCCTAGACAATTACATGGAGACTATACTGAATCCTTACATAATCAACGTCACACTAGCAGCCA
AAATGTGTAGCCAAGTGCTTTGCCAGGAGCAAGGAGTGTGTATAAGGAAAAACTGGAATTCAAGTGACTA
TCTTCACCTCAACCCAGATAATTTTGCTATTCAACTTGAGAAAGGTGGAAAGTTCACAGTACGTGGAAAA
CCGACACTTGAAGACCTGGAGCAATTTTCTGAAAAATTTTATTGCAGCTGTTATAGCACCTTGAGTTGTA
AGGAGAAAGCTGATGTAAAAGACACTGATGCTGTTGATGTGTGTATTGCTGATGGTGTCTGTATAGATGC
TTTTCTAAAACCTCCCATGGAGACAGAAGAACCTCAAATTTTCTACAATGCTTCACCCTCCACACTATCT
GCCACAATGTTCATTGTTAGTATTTTGTTTCTTATCATTTCTTCTGTAGCGAGTTTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG230203 representing NM_001174045
Red=Cloning site Green=Tags(s)

MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDM
SLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNL
GMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLL
RPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRN
RVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKS
CLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGK
PTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLS
ATMFIVSILFLIISSVASL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001174045
ORF Size 1527 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001174045.1, NP_001167516.1
RefSeq Size 2236 bp
RefSeq ORF 1530 bp
Locus ID 6677
UniProt ID P38567
Cytogenetics 7q31.32
Protein Families Druggable Genome, Transmembrane
Protein Pathways Glycosaminoglycan degradation, Metabolic pathways
Summary Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Mar 2010
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Documents
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"NM_001174045" in other vectors (4)
SKU Description Size Price
RC230203 SPAM1 (Myc-DDK-tagged)-Human sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant 4 10 ug
$289.00 $474.00
RC230203L3 Lenti-ORF clone of SPAM1 (Myc-DDK-tagged)-Human sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant 4 10 ug
$774.00
RC230203L4 Lenti-ORF clone of SPAM1 (mGFP-tagged)-Human sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant 4 10 ug
$774.00
SC328841 SPAM1 (untagged)-Human sperm adhesion molecule 1 (PH-20 hyaluronidase zona pellucida binding) (SPAM1) transcript variant 4 10 ug
$522.00

Plasmid Map for RG230203

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG230203
Circular map for RG230203

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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