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ANKRD25 (KANK2) (NM_015493) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG229386
KANK2 (tGFP-tagged) - Human KN motif and ankyrin repeat domains 2 (KANK2), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $986.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ANKRD25
Synonyms ANKRD25; MXRA3; NPHS16; PPKWH; SIP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG229386 representing NM_015493
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCAGGTCCTGCACGTGCCTGCTCCCTTCCCAGGGACCCCTGGCCCAGCCTCCCCACCTGCCTTCC
CTGCCAAGGACCCCGATCCACCCTACTCCGTGGAGACCCCCTATGGCTACCGCCTGGACCTGGACTTCCT
CAAGTACGTGGATGACATCGAGAAGGGCCACACGCTGCGACGCGTGGCAGTGCAGCGCCGCCCCCGCCTG
AGCTCGCTGCCCCGTGGCCCTGGCTCCTGGTGGACGTCCACTGAGTCGCTGTGCTCCAATGCCAGTGGGG
ACAGCCGCCACTCAGCCTATTCCTACTGCGGCCGTGGCTTCTACCCTCAGTATGGTGCTCTGGAGACCCG
CGGTGGCTTCAATCCGCGGGTGGAGCGCACGCTGCTGGATGCCCGTCGCCGTCTCGAGGACCAGGCGGCC
ACACCCACCGGCCTGGGCTCCCTGACCCCCAGTGCGGCCGGCTCGACAGCCTCCCTGGTGGGCGTGGGGT
TGCCACCCCCGACACCACGGAGTTCAGGACTGTCCACACCGGTGCCTCCCAGTGCCGGGCACCTGGCCCA
CGTGCGGGAGCAGATGGCGGGTGCCCTGCGGAAGCTGCGGCAGCTGGAGGAGCAGGTGAAGCTGATCCCT
GTGCTCCAGGTGAAGCTCTCGGTGCTCCAGGAGGAAAAGCGGCAGCTCACAGTACAACTTAAGAGCCAGA
AGTTCCTGGGCCACCCCACAGCGGGCCGGGGTCGCAGCGAGCTCTGCCTGGACCTCCCCGATCCCCCAGA
GGACCCAGTGGCACTGGAGACCCGGAGTGTGGGCACCTGGGTTCGAGAACGGGACTTGGGCATGCCTGAT
GGGGAGGCTGCCCTCGCCGCCAAGGTCGCTGTGCTGGAGACCCAGCTCAAGAAGGCGCTGCAGGAGCTGC
AGGCAGCTCAGGCCCGGCAGGCTGACCCCCAGCCCCAGGCCTGGCCACCGCCGGACAGCCCGGTCCGCGT
GGATACAGTCCGGGTGGTAGAAGGGCCACGGGAGGTGGAGGTGGTGGCCAGCACAGCCGCTGGCGCCCCC
GCACAGCGGGCCCAGAGCCTGGAGCCTTACGGCACAGGGCTGAGGGCCCTGGCAATGCCTGGTAGGCCTG
AGAGCCCACCTGTGTTCCGCAGCCAGGAGGTGGTGGAGACAATGTGCCCAGTGCCCGCTGCAGCTACCAG
CAACGTCCATATGGTGAAGAAGATTAGCATCACAGAGCGAAGCTGCGATGGAGCAGCAGGCCTCCCAGAA
GTTCCTGCCGAATCGTCTTCGTCACCCCCGGGGTCCGAGGTAGCCTCCCTTACACAGCCTGAGAAGAGCA
CAGGCCGAGTGCCCACCCAGGAGCCCACCCACAGGGAGCCCACCAGGCAAGCAGCCTCCCAAGAGTCCGA
GGAGGCCGGGGGCACCGCACCTCCGCTGTCCTCCCCTCCAGGCGGGCCCCCGGCAGGCGTGCGATCTATC
ATGAAACGGAAAGAGGAGGTTGCAGACCCCACGGCCCACCGGAGGAGCCTCCAGTTCGTGGGGGTCAACG
GCGGGTATGAGTCGTCATCCGAGGACTCCAGCACAGCAGAGAACATCTCAGACAACGACAGCACAGAGAA
CGAGGCCCCAGAGCCGAGGGAGAGGGTTCCGAGTGTGGCCGAAGCCCCCCAGCTCAGGCCTGCAGGGACG
GCAGCGGCCAAGACCAGCCGGCAGGAGTGTCAGCTGTCTCGAGAATCTCAGCACATACCCACTGCTGAGG
GGGCATCAGGATCAAACACGGAGGAGGAGATCAGGATGGAGCTAAGCCCTGACCTCATCTCAGCCTGCTT
GGCCCTGGAAAAGTACCTGGACAATCCCAACGCCCTCACAGAGCGGGAGCTGAAAGTGGCCTACACCACA
GTGCTGCAGGAGTGGCTGCGCCTGGCCTGCCGCAGCGACGCACACCCCGAGCTGGTGCGGCGGCACCTGG
TCACGTTCCGGGCCATGTCTGCGCGGCTGCTGGACTACGTGGTCAACATCGCCGACAGCAACGGCAACAC
AGCCCTGCACTACTCCGTGTCTCATGCCAACTTCCCCGTGGTGCAGCAGCTGCTCGACAGCGGTGTCTGC
AAGGTGGACAAACAGAACCGTGCTGGCTACAGCCCTATTATGCTCACCGCCCTGGCCACCCTGAAGACCC
AGGACGACATCGAGACTGTCCTTCAGCTCTTCCGGCTTGGCAACATCAATGCCAAAGCCAGCCAGGCAGG
ACAGACGGCCCTGATGCTGGCCGTCAGCCACGGGCGGGTGGACGTTGTCAAAGCCCTGCTGGCCTGTGAG
GCAGATGTCAACGTGCAAGATGATGACGGCTCCACGGCCCTCATGTGCGCCTGTGAGCACGGCCACAAGG
AGATCGCGGGGCTGCTGCTGGCCGTGCCCAGCTGTGACATCTCACTCACAGATCGCGATGGGAGCACAGC
TCTGATGGTGGCCTTGGACGCAGGGCAGAGTGAGATTGCGTCCATGCTGTATTCCCGCATGAACATCAAG
TGCTCGTTTGCCCCAATGTCAGATGACGAGAGCCCTACATCATCCTCGGCAGAAGAG

ACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG229386 representing NM_015493
Red=Cloning site Green=Tags(s)

MAQVLHVPAPFPGTPGPASPPAFPAKDPDPPYSVETPYGYRLDLDFLKYVDDIEKGHTLRRVAVQRRPRL
SSLPRGPGSWWTSTESLCSNASGDSRHSAYSYCGRGFYPQYGALETRGGFNPRVERTLLDARRRLEDQAA
TPTGLGSLTPSAAGSTASLVGVGLPPPTPRSSGLSTPVPPSAGHLAHVREQMAGALRKLRQLEEQVKLIP
VLQVKLSVLQEEKRQLTVQLKSQKFLGHPTAGRGRSELCLDLPDPPEDPVALETRSVGTWVRERDLGMPD
GEAALAAKVAVLETQLKKALQELQAAQARQADPQPQAWPPPDSPVRVDTVRVVEGPREVEVVASTAAGAP
AQRAQSLEPYGTGLRALAMPGRPESPPVFRSQEVVETMCPVPAAATSNVHMVKKISITERSCDGAAGLPE
VPAESSSSPPGSEVASLTQPEKSTGRVPTQEPTHREPTRQAASQESEEAGGTAPPLSSPPGGPPAGVRSI
MKRKEEVADPTAHRRSLQFVGVNGGYESSSEDSSTAENISDNDSTENEAPEPRERVPSVAEAPQLRPAGT
AAAKTSRQECQLSRESQHIPTAEGASGSNTEEEIRMELSPDLISACLALEKYLDNPNALTERELKVAYTT
VLQEWLRLACRSDAHPELVRRHLVTFRAMSARLLDYVVNIADSNGNTALHYSVSHANFPVVQQLLDSGVC
KVDKQNRAGYSPIMLTALATLKTQDDIETVLQLFRLGNINAKASQAGQTALMLAVSHGRVDVVKALLACE
ADVNVQDDDGSTALMCACEHGHKEIAGLLLAVPSCDISLTDRDGSTALMVALDAGQSEIASMLYSRMNIK
CSFAPMSDDESPTSSSAEE

TRPLE - GFP Tag - V
Restriction Sites SgfI-NotI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015493
ORF Size 2577 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015493.7
RefSeq Size 4966 bp
RefSeq ORF 2580 bp
Locus ID 25959
UniProt ID Q63ZY3
Cytogenetics 19p13.2
Summary This gene encodes a member of the KN motif and ankyrin repeat domains (KANK) family of proteins, which play a role in cytoskeletal formation by regulating actin polymerization. The encoded protein functions in the sequestration of steroid receptor coactivators and possibly other proteins. Mutations in this gene are associated with impaired kidney podocyte function and nephrotic syndrome, and keratoderma and woolly hair. provided by RefSeq, Jul 2016
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"NM_015493" in other vectors (6)
SKU Description Size Price
RC229386 KANK2 (Myc-DDK-tagged)-Human KN motif and ankyrin repeat domains 2 (KANK2), transcript variant 1 10 ug
$589.00 $786.00
RC229386L3 Lenti-ORF clone of KANK2 (Myc-DDK-tagged)-Human KN motif and ankyrin repeat domains 2 (KANK2), transcript variant 1 10 ug
$1,086.00
RC229386L4 Lenti-ORF clone of KANK2 (mGFP-tagged)-Human KN motif and ankyrin repeat domains 2 (KANK2), transcript variant 1 10 ug
$1,086.00
SC114656 KANK2 (untagged)-Human KN motif and ankyrin repeat domains 2 (KANK2), transcript variant 1 10 ug
$150.00
SC318330 KANK2 (untagged)-Human KN motif and ankyrin repeat domains 2 (KANK2), transcript variant 1 10 ug
$866.00
SC328021 KANK2 (untagged)-Human KN motif and ankyrin repeat domains 2 (KANK2) transcript variant 1 10 ug
$866.00

Plasmid Map for RG229386

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG229386
Circular map for RG229386

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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