MOSMO (NM_001164579) Human Tagged ORF Clone

  • TrueORF®
SKU
RG228103
C16orf52 (tGFP-tagged) - Human chromosome 16 open reading frame 52 (C16orf52)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MOSMO
Synonyms ATTHOG; BC030336; C16orf52
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG228103 representing NM_001164579
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATAAACTGACCATCATCTCAGGATGTCTCTTTCTGGCCGCCGATATCTTCGCCATCGCCAGCATCG
CCAACCCGGACTGGATCAACACCGGGGAGTCTGCGGGAGCACTCACTGTGGGCCTCGTGCGACAGTGTCA
AACAATCCATGGACGAGACCGGACGTGCATCCCTCCCCGGCTTCCCCCGGAGTGGGTCACCACACTGTTT
TTTATCATCATGGGAATCATTTCATTGACTGTCACATGTGGTTTGCTGGTGGCTTCCCACTGGCGAAGAG
AAGCTACAAAATATGCTCGATGGATAGCATTCACTGGAATGATCCTTTTCTGTATGGCTGCCCTAATATT
TCCAATAGGATTTTACATCAATGAAGTCGGAGGTCAACCTTATAAATTACCCAACAACACAGTAGTTGGG
TCATCATATGTACTTTTTGTCTTATCAATTTTCTTTACAATAGTAGGACTTCTATTTGCTGGCAAAGTTT
GTTTACCAGGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG228103 representing NM_001164579
Red=Cloning site Green=Tags(s)

MDKLTIISGCLFLAADIFAIASIANPDWINTGESAGALTVGLVRQCQTIHGRDRTCIPPRLPPEWVTTLF
FIIMGIISLTVTCGLLVASHWRREATKYARWIAFTGMILFCMAALIFPIGFYINEVGGQPYKLPNNTVVG
SSYVLFVLSIFFTIVGLLFAGKVCLPG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001164579
ORF Size 501 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001164579.2
RefSeq Size 4445 bp
RefSeq ORF 504 bp
Locus ID 730094
UniProt ID Q8NHV5
Cytogenetics 16p12.2
Summary Acts as a negative regulator of hedgehog signaling probably by promoting internalization and subsequent degradation of smoothened protein (SMO) present in the ciliary membrane. Plays a role in sonic hedgehog (SHH)-induced spinal neural progenitor cells differentiation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001164579" in other vectors (4)
SKU Description Size Price
RC228103 C16orf52 (Myc-DDK-tagged)-Human chromosome 16 open reading frame 52 (C16orf52) 10 ug
$300.00
RC228103L3 Lenti ORF clone of Human chromosome 16 open reading frame 52 (C16orf52), Myc-DDK-tagged 10 ug
$600.00
RC228103L4 Lenti ORF clone of Human chromosome 16 open reading frame 52 (C16orf52), mGFP tagged 10 ug
$600.00
SC326738 C16orf52 (untagged)-Human chromosome 16 open reading frame 52 (C16orf52) 10 ug
$330.00

Plasmid Map for RG228103

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Cloning scheme for RG228103

Circular map for RG228103

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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