FAM157A (NM_001145248) Human Tagged ORF Clone

  • TrueORF®
SKU
RG226948
FAM157A (tGFP-tagged) - Human family with sequence similarity 157, member A (FAM157A)
  $886.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FAM157A
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG226948 representing NM_001145248
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCCTTTGTTCACCACCATTCCTGGTGCCCATTCTGGTCCCATGCGTCCTCTGCCCAAGAAACACG
TAGAACCCATGGCAGTGCGACAGCTGCTTCTTGGCAACTCCACCATGATCAGGCACACTTGTCCCATGTC
TGTCCCGTTGAGCCGGCAAGTCAAGGAAGTTGCTGCCCAGAAACCAAGTGAGGACATTTACAAGAACTGG
CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACTGGATTTGCTTTTTCACCAGAGGATCC
AGATTTCCCTGTGGCCTCGCAAACAAAAAAGAAGGAAGACGGAGCAGCACAGTCATCCCTTTGTGAAAAA
GGCATTCAGGTTCTCCGCCGGCTCTGGCTGCGGGCGGCCCTCATCTAACAAGATGCTTCGGTCCATGGGT
GGAGGACAAAGGCCTACGGGCTTGGGGAGCGAGTTCTTTCGTCTCCTACATGACCTTCATCTGCTCGCGT
TTGCCATGAAGCGCATCTGGATCCACCGGAGGGGCGAGGCCACAGCTCGACCGCGAGCACCTGAGCATCC
TGCACCGCCTGCGACGGCCGTCAGGGGGCGCGATGCCGCCTCACAGAACCTCAAGCGGCGCCCGGGTTCC
GGCACAGACGGCCTGCGGCTCCAGGGGGCGGAGCCGAGCCGTCTCCTCAGGACCTACGCAGGAGGCGCCG
TCATCCCTACAGGGACACCTGAGAGGGCCCAGCCTCCTCCTCCTCAGGACCCACTCGGGAGACGCCGCTG
GCTCTCTAGGAACACCTGGGGACCGTGGCCAGGGACGACCCAGCCTCCTTCTCCTCAGCTCCTCAGGAAC
GACTGGGGAAGCTGTGGCTTCATGGTCCCCGAGGCTGCCCGAGGAAAGGTATTTCAGGACAGCCAGGAGG
GGGCGCACATCCGCCGAGAAACTGTGAGCAAGAGCGTCTGTGCTGAACCATGGCGCCACCAGAGGGCGCG
CGATCCCGCCCCAACCAACTTCCCGCTGAGGTGCCAGAAGCAGCGAGGAGCTTCAGCTTCCTCAGGGCAG
CACGAGGGTCGTGTTAACTTGGTGTTCTTCATTGGTTCCCCAACAGTTATTGCTGTACCAGATTTGCAGT
GTCCCACAAAATACTCCGGTATGTTGTAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG226948 representing NM_001145248
Red=Cloning site Green=Tags(s)

MGPLFTTIPGAHSGPMRPLPKKHVEPMAVRQLLLGNSTMIRHTCPMSVPLSRQVKEVAAQKPSEDIYKNW
QQQQQQQQQQQQQQLDLLFHQRIQISLWPRKQKRRKTEQHSHPFVKKAFRFSAGSGCGRPSSNKMLRSMG
GGQRPTGLGSEFFRLLHDLHLLAFAMKRIWIHRRGEATARPRAPEHPAPPATAVRGRDAASQNLKRRPGS
GTDGLRLQGAEPSRLLRTYAGGAVIPTGTPERAQPPPPQDPLGRRRWLSRNTWGPWPGTTQPPSPQLLRN
DWGSCGFMVPEAARGKVFQDSQEGAHIRRETVSKSVCAEPWRHQRARDPAPTNFPLRCQKQRGASASSGQ
HEGRVNLVFFIGSPTVIAVPDLQCPTKYSGMLY

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001145248
ORF Size 1149 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001145248.1, NP_001138720.1
RefSeq Size 1275 bp
RefSeq ORF 1152 bp
Locus ID 728262
Cytogenetics 3q29
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Documents
Resources
"NM_001145248" in other vectors (4)
SKU Description Size Price
RC226948 FAM157A (Myc-DDK-tagged)-Human family with sequence similarity 157, member A (FAM157A) 10 ug
$686.00
RC226948L3 Lenti ORF clone of Human family with sequence similarity 157, member A (FAM157A), Myc-DDK-tagged 10 ug
$986.00
RC226948L4 Lenti ORF clone of Human family with sequence similarity 157, member A (FAM157A), mGFP tagged 10 ug
$986.00
SC325858 FAM157A (untagged)-Human family with sequence similarity 157, member A (FAM157A) 10 ug
$732.00

Plasmid Map for RG226948

Download sequence for

Cloning scheme for RG226948

Circular map for RG226948

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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