PCBP3 (NM_001130141) Human Tagged ORF Clone

  • TrueORF®
SKU
RG225516
PCBP3 (tGFP-tagged) - Human poly(rC) binding protein 3 (PCBP3), transcript variant 2
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PCBP3
Synonyms ALPHA-CP3; PCBP3-OT1; PCBP3OT
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG225516 representing NM_001130141
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGAAGGTGACGCCTTCTGGGCCCCATCTGTCCTTCCTCACAGCACCCTCAGCACCTTAAGCCACC
ACCCTCAGCCACAATTTGGCAGAAGGATGGAGTCCAAGGTCTCAGAAGGTGGCCTGAATGTGACCCTCAC
CATCCGCCTGCTGATGCATGGAAAGGAAGTTGGAAGCATCATCGGGAAGAAAGGAGAAACTGTGAAGAAG
ATGCGTGAGGAGAGTGGTGCAAGGATCAACATCTCAGAGGGAAACTGCCCAGAGAGGATTGTGACCATCA
CAGGCCCCACAGACGCCATCTTCAAGGCCTTTGCCATGATCGCATACAAGTTTGAGGAGGATATCATCAA
CTCCATGAGCAACAGCCCTGCCACCAGCAAGCCCCCAGTGACGCTGAGGCTGGTGGTGCCTGCCAGCCAG
TGTGGGTCCCTGATCGGCAAAGGAGGCTCCAAGATCAAGGAGATCAGGGAGTCCACAGGTGCCCAGGTGC
AGGTGGCTGGGGACATGCTGCCCAACTCCACGGAGCGAGCGGTGACCATCTCGGGGACCCCAGATGCCAT
CATCCAGTGCGTCAAGCAGATCTGTGTGGTCATGCTGGAGGCCTACACAATCCAGGGACAGTATGCCATC
CCTCACCCGGATTTGACCAAGCTCCACCAGTTGGCCATGCAGCAAACCCCCTTTCCTCCCCTCGGACAGA
CCAACCCCGCTTTCCCCGGAGAAAAGCTGCCTTTACACTCCTCCGAAGAAGCTCAAAATCTGATGGGCCA
GTCATCAGGTCTGGACGCCAGCCCACCGGCCAGCACTCATGAGCTCACCATTCCCAATGATCTAATAGGC
TGCATAATTGGACGCCAAGGGACCAAAATCAATGAAATTCGACAGATGTCTGGAGCTCAGATCAAAATCG
CCAACGCCACGGAAGGGTCCTCAGAGCGTCAGATCACCATCACGGGGACCCCGGCCAACATCAGCCTTGC
CCAGTATCTCATCAACGCCAGGCTGACGTCCGAGGTCACCGGGATGGGCACGCTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG225516 representing NM_001130141
Red=Cloning site Green=Tags(s)

MGEGDAFWAPSVLPHSTLSTLSHHPQPQFGRRMESKVSEGGLNVTLTIRLLMHGKEVGSIIGKKGETVKK
MREESGARINISEGNCPERIVTITGPTDAIFKAFAMIAYKFEEDIINSMSNSPATSKPPVTLRLVVPASQ
CGSLIGKGGSKIKEIRESTGAQVQVAGDMLPNSTERAVTISGTPDAIIQCVKQICVVMLEAYTIQGQYAI
PHPDLTKLHQLAMQQTPFPPLGQTNPAFPGEKLPLHSSEEAQNLMGQSSGLDASPPASTHELTIPNDLIG
CIIGRQGTKINEIRQMSGAQIKIANATEGSSERQITITGTPANISLAQYLINARLTSEVTGMGTL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001130141
ORF Size 1035 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001130141.2
RefSeq Size 1922 bp
RefSeq ORF 1038 bp
Locus ID 54039
UniProt ID P57721
Cytogenetics 21q22.3
Summary This gene encodes a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. The protein encoded by this gene lacks the nuclear localization signals found in other subfamily members. Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Jan 2017
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Documents
Resources
"NM_001130141" in other vectors (4)
SKU Description Size Price
RC225516 PCBP3 (Myc-DDK-tagged)-Human poly(rC) binding protein 3 (PCBP3), transcript variant 2 10 ug
$457.00
RC225516L3 Lenti ORF clone of Human poly(rC) binding protein 3 (PCBP3), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC225516L4 Lenti ORF clone of Human poly(rC) binding protein 3 (PCBP3), transcript variant 2, mGFP tagged 10 ug
$757.00
SC324976 PCBP3 (untagged)-Human poly(rC) binding protein 3 (PCBP3), transcript variant 2 10 ug
$503.00

Plasmid Map for RG225516

Download sequence for

Cloning scheme for RG225516

Circular map for RG225516

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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