RNASE9 (NM_001110356) Human Tagged ORF Clone

  • TrueORF®
SKU
RG225233
RNASE9 (tGFP-tagged) - Human ribonuclease, RNase A family, 9 (non-active) (RNASE9), transcript variant 5
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RNASE9
Synonyms h461; HEL128; RAK1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG225233 representing NM_001110356
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTATTGCCGCAGCAGCTGCTGCAGCTGG
TGCAGTTTCAAGAGGTGGATACAGATTTTGATTTCCCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTG
TTTGGAAAAATTTTTTAGTACAGGGCCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTT
ATTGAACCTGGAATGCCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACT
ACAAACACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATCTGTTA
CAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGTCTTGTAGAAGGAGTG
TATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATACGAATCACTTTATAGGAAGGGCTACG
TCCTTATCACTTGTTCATGGCAAAATGAAATGCAAAAACGTATTCCTCATACTATAAATGATCTCGTGGA
GCCACCTGAACACAGAAGTTTCCTCAGTGAGGATGGTGTCTTTGTCATATCGCCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG225233 representing NM_001110356
Red=Cloning site Green=Tags(s)

MMRTLITTHPLPLLLLPQQLLQLVQFQEVDTDFDFPEEDKKEEFEECLEKFFSTGPARPPTKEKVKRRVL
IEPGMPLNHIEYCNHEIMGKNVYYKHRWVAEHYFLLMQYDELQKICYNRFVPCKNGIRKCNRSKGLVEGV
YCNLTEAFEIPACKYESLYRKGYVLITCSWQNEMQKRIPHTINDLVEPPEHRSFLSEDGVFVISP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001110356
ORF Size 615 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001110356.1, NP_001103826.1
RefSeq Size 1120 bp
RefSeq ORF 618 bp
Locus ID 390443
UniProt ID P60153
Cytogenetics 14q11.2
Protein Families Secreted Protein
Summary Does not exhibit any ribonuclease activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001110356" in other vectors (4)
SKU Description Size Price
RC225233 RNASE9 (Myc-DDK-tagged)-Human ribonuclease, RNase A family, 9 (non-active) (RNASE9), transcript variant 5 10 ug
$300.00
RC225233L3 Lenti ORF clone of Human ribonuclease, RNase A family, 9 (non-active) (RNASE9), transcript variant 5, Myc-DDK-tagged 10 ug
$600.00
RC225233L4 Lenti ORF clone of Human ribonuclease, RNase A family, 9 (non-active) (RNASE9), transcript variant 5, mGFP tagged 10 ug
$600.00
SC317184 RNASE9 (untagged)-Human ribonuclease, RNase A family, 9 (non-active) (RNASE9), transcript variant 5 10 ug
$330.00

Plasmid Map for RG225233

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Cloning scheme for RG225233

Circular map for RG225233

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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