MAGEB1 (NM_177404) Human Tagged ORF Clone

SKU RG224906

MAGEB1 (tGFP-tagged) - Human melanoma antigen family B, 1 (MAGEB1), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: MAGEB1
• Synonyms: CT3.1; DAM10; MAGE-Xp; MAGEL1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224906 representing NM_177404; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCCTCGGGGTCAGAAGAGTAAGCTCCGTGCTCGTGAGAAACGCCGCAAGGCGCGAGAGGAGACCCACT; ATCTCAAGGTTGCTCACGCCACTGCAGCAGAGAAAGAGGAGTGCCCCTCCTCCTCTCCTGTTTTAGGGGA; TACTCCCACAAGCTCCCCTGCTGCTGGCATTCCCCAGAAGCCTCAGGGAGCTCCACCCACCACCACTGCT; GCTGCAGCTGTGTCATGTACCGAATCTGACGAAGGTGCCAAATGCCAAGGTGAGGAAAATGCAAGTTTCT; CCCAGGCCACAACATCCACTGAGAGCTCAGTCAAAGATCCTGTAGCCTGGGAGGCAGGAATGCTGATGCA; CTTCATTCTACGTAAGTATAAAATGAGAGAGCCCATTATGAAGGCAGATATGCTGAAGGTTGTTGATGAA; AAGTACAAGGAGTACTTCACTGAGATCCTCAATGGAGCCTCTCGCCGCTTGGAGCTCGTCTTTGGCCTTG; ATTTGAAGGAAGACAACCCTAGTGGCCACACCTACACCCTCGTCAGTAAGCTAAACCTCACCAATGATGG; AAACCTGAGCAATGATTGGGACTTTCCCAGGAATGGGCTTCTGATGCCTCTCCTGGGTGTGATCTTCTTA; AAGGGCAACTCTGCCACCGAGGAAGAGATCTGGAAATTCATGAATGTGTTGGGAGCCTATGATGGAGAGG; AGCACTTAATCTATGGGGAACCCCGTAAGTTCATCACCCAAGATCTGGTGCAGGAAAAATATCTGAAGTA; CGAGCAGGTGCCCAACAGTGATCCCCCACGCTATCAATTCCTATGGGGTCCGAGAGCCTATGCTGAAACC; ACCAAGATGAAAGTCCTCGAGTTTTTGGCCAAGATGAATGGTGCCACTCCCCGTGACTTCCCATCCCATT; ATGAAGAGGCTTTGAGAGATGAGGAAGAGAGAGCCCAAGTCCGATCCAGTGTTAGAGCCAGGCGTCGCAC; TACTGCCACGACTTTTAGAGCGCGTTCTAGAGCCCCATTCAGCAGGTCCTCCCACCCCATG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224906 representing NM_177404; Red=Cloning site Green=Tags(s); ; MPRGQKSKLRAREKRRKAREETHYLKVAHATAAEKEECPSSSPVLGDTPTSSPAAGIPQKPQGAPPTTTA; AAAVSCTESDEGAKCQGEENASFSQATTSTESSVKDPVAWEAGMLMHFILRKYKMREPIMKADMLKVVDE; KYKEYFTEILNGASRRLELVFGLDLKEDNPSGHTYTLVSKLNLTNDGNLSNDWDFPRNGLLMPLLGVIFL; KGNSATEEEIWKFMNVLGAYDGEEHLIYGEPRKFITQDLVQEKYLKYEQVPNSDPPRYQFLWGPRAYAET; TKMKVLEFLAKMNGATPRDFPSHYEEALRDEEERAQVRSSVRARRRTTATTFRARSRAPFSRSSHPM; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177404
• ORF Size: 1041 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_177404.1, NP_796379.1
• RefSeq Size: 1722 bp
• RefSeq ORF: 1044 bp
• Locus ID: 4112
• UniProt ID: P43366
• Cytogenetics: Xp21.2
• Summary: This gene is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. This gene is localized in the DSS (dosage-sensitive sex reversal) critical region, and expressed in testis and in a significant fraction of tumors of various histological types. This gene and other MAGEB members are clustered on chromosome Xp22-p21. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene, however, the full length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]