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LRRC4B (NM_001080457) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG224845
LRRC4B (tGFP-tagged) - Human leucine rich repeat containing 4B (LRRC4B)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$789.00 $853.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LRRC4B
Synonyms HSM; LRIG4; NGL-3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG224845 representing NM_001080457
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCGTGCCCGCGGCTCCCCGTGCCCCCCGCTGCCGCCCGGTAGGATGTCCTGGCCCCACGGGGCAT
TGCTCTTCCTCTGGCTCTTCTCCCCACCCCTGGGGGCCGGTGGAGGTGGAGTGGCCGTGACGTCTGCCGC
CGGAGGGGGCTCCCCGCCGGCCACCTCCTGCCCCGTGGCCTGCTCCTGCAGCAACCAGGCCAGCCGGGTG
ATCTGCACACGGAGAGACCTGGCCGAGGTCCCAGCCAGCATCCCGGTCAACACGCGGTACCTGAACCTGC
AAGAGAACGGCATCCAGGTGATCCGGACGGACACGTTCAAGCACCTGCGGCACCTGGAGATTCTGCAGCT
GAGCAAGAACCTGGTGCGCAAGATCGAGGTGGGCGCCTTCAACGGGCTGCCCAGCCTCAACACGCTGGAG
CTTTTTGACAACCGGCTGACCACGGTGCCCACGCAGGCCTTCGAGTACCTGTCCAAGCTGCGGGAGCTCT
GGCTGCGGAACAACCCCATCGAGAGCATCCCCTCCTACGCCTTCAACCGCGTGCCCTCGCTGCGGCGCCT
GGACCTGGGCGAGCTCAAGCGGCTGGAATACATCTCGGAGGCGGCCTTCGAGGGGCTGGTCAACCTGCGC
TACCTCAACCTGGGCATGTGCAACCTCAAGGACATCCCCAACCTGACGGCCCTGGTGCGCCTGGAGGAGC
TGGAGCTGTCGGGCAACCGGCTGGACCTGATCCGCCCGGGCTCCTTCCAGGGTCTCACCAGCCTGCGCAA
GCTGTGGCTCATGCACGCCCAGGTAGCCACCATCGAGCGCAACGCCTTCGACGACCTCAAGTCGCTGGAG
GAGCTCAACCTGTCCCACAACAACCTGATGTCGCTGCCCCACGACCTCTTCACGCCCCTGCACCGCCTCG
AGCGCGTGCACCTCAACCACAACCCCTGGCATTGCAACTGCGACGTGCTCTGGCTGAGCTGGTGGCTCAA
GGAGACGGTGCCCAGCAACACGACGTGCTGCGCCCGCTGTCATGCGCCCGCCGGCCTCAAGGGGCGCTAC
ATTGGGGAGCTGGACCAGTCGCATTTCACCTGCTATGCGCCCGTCATCGTGGAGCCGCCCACGGACCTCA
ACGTCACCGAGGGCATGGCTGCCGAGCTCAAATGCCGCACGGGCACCTCCATGACCTCCGTCAACTGGCT
GACGCCCAACGGCACCCTCATGACCCACGGCTCCTACCGCGTGCGCATCTCCGTCCTGCATGACGGCACG
CTTAACTTCACCAACGTCACCGTGCAGGACACGGGCCAGTACACGTGCATGGTGACGAACTCAGCCGGCA
ACACCACCGCCTCGGCCACGCTCAACGTCTCGGCCGTGGACCCCGTGGCGGCCGGGGGCACCGGCAGCGG
CGGGGGCGGCCCTGGGGGCAGTGGTGGTGTTGGAGGGGGCAGTGGCGGCTACACCTACTTCACCACGGTG
ACCGTGGAGACCCTGGAGACGCAGCCCGGAGAGGAGGCCCTGCAGCCGCGGGGGACGGAGAAGGAACCGC
CAGGGCCCACGACAGACGGTGTCTGGGGTGGGGGCCGGCCTGGGGACGCGGCCGGCCCTGCCTCGTCTTC
TACCACGGCACCCGCCCCGCGCTCCTCGCGGCCCACGGAGAAGGCGTTCACGGTGCCCATCACGGATGTG
ACGGAGAACGCCCTCAAGGACCTGGACGACGTCATGAAGACCACCAAAATCATCATCGGCTGCTTCGTGG
CCATCACGTTCATGGCCGCGGTGATGCTCGTGGCCTTCTACAAGCTGCGCAAGCAGCACCAGCTCCACAA
GCACCACGGGCCCACGCGCACCGTGGAGATCATCAACGTGGAGGACGAGCTGCCCGCCGCCTCGGCCGTG
TCCGTGGCCGCCGCGGCCGCCGTGGCCAGTGGGGGTGGTGTGGGCGGGGACAGCCACCTGGCCCTGCCCG
CCCTGGAGCGAGACCACCTCAACCACCACCACTACGTGGCTGCCGCCTTCAAGGCGCACTACAGCAGCAA
CCCCAGCGGCGGGGGCTGCGGGGGCAAAGGCCCGCCTGGCCTCAACTCCATCCACGAACCTCTGCTCTTC
AAGAGCGGCTCCAAGGAGAACGTGCAAGAGACGCAGATC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG224845 representing NM_001080457
Red=Cloning site Green=Tags(s)

MARARGSPCPPLPPGRMSWPHGALLFLWLFSPPLGAGGGGVAVTSAAGGGSPPATSCPVACSCSNQASRV
ICTRRDLAEVPASIPVNTRYLNLQENGIQVIRTDTFKHLRHLEILQLSKNLVRKIEVGAFNGLPSLNTLE
LFDNRLTTVPTQAFEYLSKLRELWLRNNPIESIPSYAFNRVPSLRRLDLGELKRLEYISEAAFEGLVNLR
YLNLGMCNLKDIPNLTALVRLEELELSGNRLDLIRPGSFQGLTSLRKLWLMHAQVATIERNAFDDLKSLE
ELNLSHNNLMSLPHDLFTPLHRLERVHLNHNPWHCNCDVLWLSWWLKETVPSNTTCCARCHAPAGLKGRY
IGELDQSHFTCYAPVIVEPPTDLNVTEGMAAELKCRTGTSMTSVNWLTPNGTLMTHGSYRVRISVLHDGT
LNFTNVTVQDTGQYTCMVTNSAGNTTASATLNVSAVDPVAAGGTGSGGGGPGGSGGVGGGSGGYTYFTTV
TVETLETQPGEEALQPRGTEKEPPGPTTDGVWGGGRPGDAAGPASSSTTAPAPRSSRPTEKAFTVPITDV
TENALKDLDDVMKTTKIIIGCFVAITFMAAVMLVAFYKLRKQHQLHKHHGPTRTVEIINVEDELPAASAV
SVAAAAAVASGGGVGGDSHLALPALERDHLNHHHYVAAAFKAHYSSNPSGGGCGGKGPPGLNSIHEPLLF
KSGSKENVQETQI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001080457
ORF Size 2139 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001080457.2
RefSeq Size 2958 bp
RefSeq ORF 2142 bp
Locus ID 94030
UniProt ID Q9NT99
Cytogenetics 19q13.33
Protein Families Druggable Genome, Transmembrane
Summary Synaptic adhesion protein. Regulates the formation of excitatory synapses. The trans-synaptic adhesion between LRRC4B and PTPRF regulates the formation of excitatory synapses in a bidirectional manner (By similarity).UniProtKB/Swiss-Prot Function
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"NM_001080457" in other vectors (6)
SKU Description Size Price
RC224845 LRRC4B (Myc-DDK-tagged)-Human leucine rich repeat containing 4B (LRRC4B) 10 ug
$653.00
RC224845L1 Lenti ORF clone of Human leucine rich repeat containing 4B (LRRC4B), Myc-DDK-tagged 10 ug
$953.00
RC224845L2 Lenti ORF clone of Human leucine rich repeat containing 4B (LRRC4B), mGFP tagged 10 ug
$953.00
RC224845L3 Lenti ORF clone of Human leucine rich repeat containing 4B (LRRC4B), Myc-DDK-tagged 10 ug
$953.00
RC224845L4 Lenti ORF clone of Human leucine rich repeat containing 4B (LRRC4B), mGFP tagged 10 ug
$953.00
SC315826 LRRC4B (untagged)-Human leucine rich repeat containing 4B (LRRC4B) 10 ug
$719.00

Plasmid Map for RG224845

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG224845
Circular map for RG224845

file-alt Citations

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