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EDG7 (LPAR3) (NM_012152) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG223706
LPAR3 (tGFP-tagged) - Human lysophosphatidic acid receptor 3 (LPAR3)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EDG7
Synonyms Edg-7; EDG7; GPCR; HOFNH30; LP-A3; LPA3; RP4-678I3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG223706 representing NM_012152
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGAGTGTCACTATGACAAGCACATGGACTTTTTTTATAATAGGAGCAACACTGATACTGTCGATG
ACTGGACAGGAACAAAGCTTGTGATTGTTTTGTGTGTTGGGACGTTTTTCTGCCTGTTTATTTTTTTTTC
TAATTCTCTGGTCATCGCGGCAGTGATCAAAAACAGAAAATTTCATTTCCCCTTCTACTACCTGTTGGCT
AATTTAGCTGCTGCCGATTTCTTCGCTGGAATTGCCTATGTATTCCTGATGTTTAACACAGGCCCAGTTT
CAAAAACTTTGACTGTCAACCGCTGGTTTCTCCGTCAGGGGCTTCTGGACAGTAGCTTGACTGCTTCCCT
CACCAACTTGCTGGTTATCGCCGTGGAGAGGCACATGTCAATCATGAGGATGCGGGTCCATAGCAACCTG
ACCAAAAAGAGGGTGACACTGCTCATTTTGCTTGTCTGGGCCATCGCCATTTTTATGGGGGCGGTCCCCA
CACTGGGCTGGAATTGCCTCTGCAACATCTCTGCCTGCTCTTCCCTGGCCCCCATTTACAGCAGGAGTTA
CCTTGTTTTCTGGACAGTGTCCAACCTCATGGCCTTCCTCATCATGGTTGTGGTGTACCTGCGGATCTAC
GTGTACGTCAAGAGGAAAACCAACGTCTTGTCTCCGCATACAAGTGGGTCCATCAGCCGCCGGAGGACAC
CCATGAAGCTAATGAAGACGGTGATGACTGTCTTAGGGGCGTTTGTGGTATGCTGGACCCCGGGCCTGGT
GGTTCTGCTCCTCGACGGCCTGAACTGCAGGCAGTGTGGCGTGCAGCATGTGAAAAGGTGGTTCCTGCTG
CTGGCGCTGCTCAACTCCGTCGTGAACCCCATCATCTACTCCTACAAGGACGAGGACATGTATGGCACCA
TGAAGAAGATGATCTGCTGCTTCTCTCAGGAGAACCCAGAGAGGCGTCCCTCTCGCATCCCCTCCACAGT
CCTCAGCAGGAGTGACACAGGCAGCCAGTACATAGAGGATAGTATTAGCCAAGGTGCAGTCTGCAATAAA
AGCACTTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG223706 representing NM_012152
Red=Cloning site Green=Tags(s)

MNECHYDKHMDFFYNRSNTDTVDDWTGTKLVIVLCVGTFFCLFIFFSNSLVIAAVIKNRKFHFPFYYLLA
NLAAADFFAGIAYVFLMFNTGPVSKTLTVNRWFLRQGLLDSSLTASLTNLLVIAVERHMSIMRMRVHSNL
TKKRVTLLILLVWAIAIFMGAVPTLGWNCLCNISACSSLAPIYSRSYLVFWTVSNLMAFLIMVVVYLRIY
VYVKRKTNVLSPHTSGSISRRRTPMKLMKTVMTVLGAFVVCWTPGLVVLLLDGLNCRQCGVQHVKRWFLL
LALLNSVVNPIIYSYKDEDMYGTMKKMICCFSQENPERRPSRIPSTVLSRSDTGSQYIEDSISQGAVCNK
STS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012152
ORF Size 1059 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012152.3
RefSeq Size 1148 bp
RefSeq ORF 1062 bp
Locus ID 23566
UniProt ID Q9UBY5
Cytogenetics 1p22.3
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
Summary This gene encodes a member of the G protein-coupled receptor family, as well as the EDG family of proteins. This protein functions as a cellular receptor for lysophosphatidic acid and mediates lysophosphatidic acid-evoked calcium mobilization. This receptor couples predominantly to G(q/11) alpha proteins. provided by RefSeq, Jul 2008
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"NM_012152" in other vectors (6)
SKU Description Size Price
RC223706 LPAR3 (Myc-DDK-tagged)-Human lysophosphatidic acid receptor 3 (LPAR3) 10 ug
$457.00
RC223706L1 Lenti ORF clone of Human lysophosphatidic acid receptor 3 (LPAR3), Myc-DDK-tagged 10 ug
$757.00
RC223706L2 Lenti ORF clone of Human lysophosphatidic acid receptor 3 (LPAR3), mGFP tagged 10 ug
$757.00
RC223706L3 Lenti ORF clone of Human lysophosphatidic acid receptor 3 (LPAR3), Myc-DDK-tagged 10 ug
$757.00
RC223706L4 Lenti ORF clone of Human lysophosphatidic acid receptor 3 (LPAR3), mGFP tagged 10 ug
$757.00
SC115507 LPAR3 (untagged)-Human lysophosphatidic acid receptor 3 (LPAR3) 10 ug
$457.00

Plasmid Map for RG223706

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG223706
Circular map for RG223706

file-alt Citations

Discovery of PIPE-791 , A Potent and Brain-Penetrant Lysophosphatidic Acid Receptor 1 Antagonist with Slow Tight Binding Characteristics for the Treatment of Neuroinflammatory Disorders
Chen, Baccei, Baccei et al
J Med Chem (2026)
AI Summary: OriGene’s human LPAR1–6 plasmids (Cat. Nos. RG206066, RG206444, RG223706, RG223927, RG209907, RG207345) were used to transiently express LPAR receptors in HEK293T cells, enabling the evaluation of PIPE-791’s antagonistic activity against LPA-induced calcium mobilization in the study.
Snippet: …RG206066), LPAR2 (Origene, Cat. … RG206444), LPAR3 (Origene, Cat. … RG223706), LPAR4 (Origene, Cat. … RG223927), LPAR5 (Origene, Cat. … RG209907), and LPAR6 (Origene, Cat. … RG207345) were maxiprepped at Eton Biosciences (San Diego, CA, USA). …
Showing 1-1 of 1 papers.
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