OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

CACNA1S (NM_000069) Human Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
RG223574
CACNA1S (tGFP-tagged) - Human calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK
  $2,560.00
2 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA308209 Rabbit polyclonal antibody to CACNA1S (calcium channel, voltage-dependent, L type, alpha 1S subunit)
100 ul
$665.00
TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8 100 ul
$447.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "CACNA1S or NM_000069"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CACNA1S
Synonyms CACNL1A3; Cav1.1; CCHL1A3; HOKPP; HOKPP1; hypoPP; MHS5; TTPP1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000069
ORF Size 5619 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000069.1, NP_000060.1
RefSeq Size 6160 bp
RefSeq ORF 5622 bp
Locus ID 779
UniProt ID Q13698
Cytogenetics 1q32.1
Protein Families Druggable Genome, Ion Channels: Calcium, Transmembrane
Protein Pathways Alzheimer's disease, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Calcium signaling pathway, Cardiac muscle contraction, Dilated cardiomyopathy, GnRH signaling pathway, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Vascular smooth muscle contraction
Summary This gene encodes one of the five subunits of the slowly inactivating L-type voltage-dependent calcium channel in skeletal muscle cells. Mutations in this gene have been associated with hypokalemic periodic paralysis, thyrotoxic periodic paralysis and malignant hyperthermia susceptibility. provided by RefSeq, Jul 2008
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_000069" in other vectors (3)
SKU Description Size Price
RC223574 CACNA1S (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S) 10 ug
$2,360.00
RC223574L1 Lenti ORF clone of Human calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), Myc-DDK-tagged 10 ug
$2,660.00
SC300005 CACNA1S (untagged)-Human calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S) 10 ug
$2,519.00

Plasmid Map for RG223574

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG223574
Circular map for RG223574

file-alt Citations

Na leak with gating pore properties in hypokalemic periodic paralysis V876E mutant muscle Ca channel
Fuster, Perrot, Berthier et al
J. Gen. Physiol. (2017) 149 (12), 1139-1148 DOI: 10.1085/jgp.201711834
Cited by 25
AI Summary: OriGene’s turboGFP-tagged human Cav1.1 plasmids were used to express both wild-type and V876E mutant channels in mouse muscle, enabling the study of their localization and functional properties in hypokalemic periodic paralysis research.
Snippet: Injected plasmids encoded for turboGFP-tagged WT or V876E human Cav1.1 (Origene)
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products