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FLJ42957 (NM_207436) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG223547
FLJ42957 (tGFP-tagged) - Human FLJ42957 protein (FLJ42957)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $489.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FLJ42957
Synonyms MGC148154, MGC148155
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG223547 representing NM_207436
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTTCTCCCCGTACAGCACGATGATTACAGTCTGTGTTTGTTTCAACAGTCGTGTACAACTGACAG
TGCCATCATTTACTGCCTGGCTCAGGTCACGTTACTCTAAGGCTTTATTTATGGTGTTACGAAGGGCAGC
ACAGGAAAAGGACAAGGGTGTCTGTCAGGGATGGCACTGTGTTAAAAAGTGGGCGTGCAAGGGCCGCATT
CCCGGGCAGCCGCTGCAACCTCAGCCCCTGGGCCCTTACCTCCGCAGCCTCTCCCAGCATCCAGCTACCC
AGACTCCAAGGCCCCAGGCGAGAGCCAGCTCTCGGTACCTGGAGCTCCACAGGTCCCAGAATCGGGGTGG
ATCAGAGTTCAAATTCTGGTTCTGCTACTGTCTAATTGCGTGCTGCAGGGACTCAATCTCTTCATCTGGG
AAATGGGAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG223547 representing NM_207436
Red=Cloning site Green=Tags(s)

MSFSPYSTMITVCVCFNSRVQLTVPSFTAWLRSRYSKALFMVLRRAAQEKDKGVCQGWHCVKKWACKGRI
PGQPLQPQPLGPYLRSLSQHPATQTPRPQARASSRYLELHRSQNRGGSEFKFWFCYCLIACCRDSISSSG
KWE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_207436
ORF Size 429 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_207436.1, NP_997319.1
RefSeq Size 1597 bp
RefSeq ORF 431 bp
Locus ID 400077
Cytogenetics 12q24.22
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"NM_207436" in other vectors (3)
SKU Description Size Price
RC223547 FLJ42957 (Myc-DDK-tagged)-Human FLJ42957 protein (FLJ42957) 10 ug
$289.00
RC223547L3 Lenti ORF clone of Human FLJ42957 protein (FLJ42957), Myc-DDK-tagged 10 ug
$450.00
RC223547L4 Lenti ORF clone of Human FLJ42957 protein (FLJ42957), mGFP tagged 10 ug
$450.00

Plasmid Map for RG223547

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Cloning scheme for RG223547
Circular map for RG223547

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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