WNK4 (NM_032387) Human Tagged ORF Clone
$1,282.00
3 Weeks*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | WNK4 |
| Synonyms | PHA2B; PRKWNK4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_032387 |
| ORF Size | 3729 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_032387.5 |
| RefSeq Size | 4147 bp |
| RefSeq ORF | 3732 bp |
| Locus ID | 65266 |
| UniProt ID | Q96J92 |
| Cytogenetics | 17q21.2 |
| Protein Families | Druggable Genome, Protein Kinase |
| Summary | This gene encodes a member of the WNK family of serine-threonine protein kinases. The kinase is part of the tight junction complex in kidney cells, and regulates the balance between NaCl reabsorption and K(+) secretion. The kinase regulates the activities of several types of ion channels, cotransporters, and exchangers involved in electrolyte flux in epithelial cells. Mutations in this gene result in pseudohypoaldosteronism type IIB.provided by RefSeq, Sep 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_032387" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC223269 | WNK4 (Myc-DDK-tagged)-Human WNK lysine deficient protein kinase 4 (WNK4) | 10 ug |
$1,082.00
|
|
| RC223269L1 | Lenti ORF clone of Human WNK lysine deficient protein kinase 4 (WNK4), Myc-DDK-tagged | 10 ug |
$1,382.00
|
|
| RC223269L2 | Lenti ORF clone of Human WNK lysine deficient protein kinase 4 (WNK4), mGFP tagged | 10 ug |
$1,382.00
|
|
| RC223269L3 | Lenti ORF clone of Human WNK lysine deficient protein kinase 4 (WNK4), Myc-DDK-tagged | 10 ug |
$1,382.00
|
|
| RC223269L4 | Lenti ORF clone of Human WNK lysine deficient protein kinase 4 (WNK4), mGFP tagged | 10 ug |
$1,382.00
|
|
| SC305438 | WNK4 (untagged)-Human WNK lysine deficient protein kinase 4 (WNK4) | 10 ug |
$1,191.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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