CILP2 (NM_153221) Human Tagged ORF Clone

SKU
RG223164
CILP2 (tGFP-tagged) - Human cartilage intermediate layer protein 2 (CILP2)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$1,709.00
3 Weeks*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CILP2
Synonyms CLIP-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG223164 representing NM_153221
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCGCTGCTGCCACTGCTCTGTCTCTGTGTCGTCGCTGCGCACCTGGCGGGGGCCCGAGACGCCA
CCCCCACCGAGGAGCCAATGGCGACTGCACTGGGCCTGGAAAGACGGTCCGTGTACACCGGCCAGCCCTC
ACCAGCCCTGGAGGACTGGGAAGAGGCCAGCGAGTGGACGTCCTGGTTCAACGTGGACCACCCCGGAGGC
GACGGCGACTTCGAGAGCCTGGCTGCCATCCGCTTCTACTACGGGCCAGCGCGCGTGTGCCCGCGACCGC
TGGCGCTGGAAGCGCGCACCACGGACTGGGCCCTGCCGTCCGCCGTCGGCGAGCGCGTGCACTTGAACCC
CACGCGCGGCTTCTGGTGCCTCAACCGCGAGCAACCGCGTGGCCGCCGCTGCTCCAACTACCACGTGCGC
TTCCGCTGCCCACTAGAAGCCTCGTGGGGCGCGTGGGGCCCGTGGGGTCCCTGCTCGGGGAGCTGTGGGC
CAGGCCGTCGCTTGCGCCGCCGCCACTGCCCAAGCCCCGCTGGGGATGCGTGTCCCGGGCGTCCTCTGGA
GGCGCAGAAGTGCGTGCGGCCTCGGTGTCCAGGGTGCAGCCTTGACACCTGTGAATGCCCGGACCACATC
CTCCTGGGCTCGGTGGTCACCCCATCTGGGCAACCACTGCTAGGAGCCAGGGTCTCCCTGCGAGACCAGC
CTGGCACTGTGGCCACCAGCGATGCTCACGGAACCTTCCGGGTGCCTGGTGTCTGTGCTGACAGCCGCGC
CAACATCAGGGCCCAGATGGATGGCTTCTCTGCAGGGGAGGCCCAGGCCCAGGCCAACGGATCCATCTCT
GTGGTCACCATCATCCTTGATAAGTTGGAGAAGCCGTACCTGGTGAAACACCCTGAGTCCCGAGTGCGAG
AGGCTGGCCAGAATGTGACTTTCTGCTGCAAAGCCTCCGGGACCCCCATGCCCAAGAAATACTCCTGGTT
CCACAATGGGACCCTGCTGGACAGGCGAGCTCATGGGTACGGGGCCCACCTGGAGCTGCGGGGACTGCGC
CCAGACCAGGCTGGCATCTACCACTGCAAGGCATGGAATGAGGCGGGTGCCGTGCGCTCGGGCACTGCCC
GGCTCACTGTACTTGCCCCAGGCCAGCCAGCCTGCGACCCCCGGCCCCGAGAGTACCTGATCAAGCTCCC
TGAGGACTGTGGTCAGCCAGGTAGTGGCCCTGCCTACCTGGATGTGGGCCTCTGTCCCGACACCCGCTGC
CCCAGCCTGGCAGGCTCCAGCCCCCGCTGCGGGGACGCCAGCTCCCGCTGCTGCTCTGTGCGCCGTCTGG
AGAGAAGGGAGATTCACTGCCCTGGCTACGTCCTCCCAGTGAAGGTGGTGGCAGAGTGTGGCTGCCAGAA
GTGTCTGCCCCCTCGGGGGCTGGTCCGGGGCCGTGTTGTGGCTGCTGACTCCGGGGAGCCGCTACGCTTC
GCCAGGATTCTGCTGGGCCAGGAGCCCATCGGCTTCACCGCCTACCAGGGCGACTTTACCATTGAGGTGC
CGCCCTCCACCCAGCGGCTGGTGGTGACTTTTGTGGACCCCAGCGGTGAGTTCATGGACGCTGTCCGGGT
CTTGCCTTTTGATCCTCGAGGTGCCGGCGTGTACCACGAGGTCAAGGCCATGCGGAAGAAAGCCCCGGTC
ATTTTACATACCAGCCAGAGCAACACGATCCCCCTGGGCGAGCTGGAAGATGAGGCGCCCCTGGGCGAGC
TGGTCCTGCCTTCTGGCGCTTTCCGCAGAGCCGACGGCAAACCCTACTCGGGGCCTGTGGAGGCCCGGGT
GACGTTCGTGGACCCCCGAGACCTCACCTCGGCGGCGTCTGCCCCCAGTGACCTGCGCTTCGTGGACAGC
GACGGCGAGCTGGCTCCACTGCGCACCTACGGCATGTTCTCCGTGGACCTCCGTGCGCCCGGCTCCGCGG
AGCAGCTGCAGGTGGGGCCGGTGGCCGTGCGGGTGGCCGCCAGCCAGATCCACATGCCAGGCCACGTGGA
GGCCCTCAAGCTGTGGTCGCTGAACCCCGAGACCGGGTTGTGGGAGGAGGAGAGCGGCTTCCGGCGCGAG
GGGTCCTCGGGCCCCCGGGTGCGCCGGGAGGAGCGCGTCTTCCTGGTGGGCAACGTGGAGATCCGGGAGC
GGCGCCTGTTCAATCTGGACGTGCCTGAGCGCCGCCGCTGCTTCGTGAAGGTGCGCGCCTACGCCAACGA
CAAGTTCACCCCCAGCGAGCAGGTGGAGGGCGTGGTGGTCACGCTGGTCAATCTGGAGCCCGGCCCCGGC
TTCTCCGCCAACCCCCGTGCCTGGGGCCGCTTTGACAGCGCGGTCACCGGCCCCAATGGCGCCTGCCTCC
CCGCCTTCTGCGACGCCGACAGGCCAGACGCCTACACCGCCCTGGTCACCGCCACCCTGGGCGGCGAGGA
GCTGGAGCCGGCCCCTTCCTTGCCCCGCCCACTCCCGGCCACCGTGGGCGTCACCCAGCCCTACCTGGAC
AGGCTGGGGTACCGTCGGACGGACCACGACGATCCCGCCTTCAAGCGTAACGGCTTCCGCATCAACCTCG
CCAAGCCCAGGCCAGGTGACCCCGCCGAGGCCAATGGGCCTGTGTACCCGTGGCGCAGCCTGCGGGAATG
CCAGGGGGCCCCGGTGACTGCCAGCCACTTCCGCTTCGCCAGGGTGGAGGCGGACAAGTACGAGTACAAC
GTGGTCCCCTTCCGAGAGGGCACACCTGCCTCCTGGACTGGCGATCTCCTGGCCTGGTGGCCCAACCCGC
AGGAGTTCCGGGCCTGCTTCCTCAAGGTGAAGATCCAGGGTCCCCAGGAGTATATGGTCCGCTCCCACAA
CGCAGGGGGCAGCCACCCACGCACCCGCGGCCAGCTCTACGGACTTCGGGATGCCCGGAGTGTGCGAGAC
CCCGAGCGTCCGGGCACCTCGGCAGCCTGCGTGGAGTTCAAGTGCAGCGGGATGCTGTTCGACCAGCGGC
AGGTGGACAGGACGCTGGTGACCATTATGCCCCAGGGCAGCTGCCGGCGCGTGGCCGTCAACGGACTCCT
TCGGGATTACCTGACCCGGCACCCCCCACCGGTGCCCGCGGAGGACCCAGCTGCCTTCTCCATGCTGGCC
CCCCTAGACCCTCTGGGCCACAACTATGGCGTCTACACTGTCACTGACCAGAGCCCACGCTTGGCCAAGG
AGATCGCCATTGGCCGCTGCTTTGATGGTTCCTCTGACGGCTTCTCCAGAGAGATGAAGGCTGATGCCGG
CACAGCCGTCACCTTCCAGTGCCGGGAGCCACCGGCCGGACGACCCAGCCTCTTCCAGAGGCTGCTGGAG
TCCCCGGCGACAGCACTTGGTGACATCCGCAGGGAGATGAGCGAGGCGGCGCAGGCACAGGCCCGGGCCT
CAGGTCCCCTCCGCACCCGCCGGGGTAGGGTCCGGCAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG223164 representing NM_153221
Red=Cloning site Green=Tags(s)

MASLLPLLCLCVVAAHLAGARDATPTEEPMATALGLERRSVYTGQPSPALEDWEEASEWTSWFNVDHPGG
DGDFESLAAIRFYYGPARVCPRPLALEARTTDWALPSAVGERVHLNPTRGFWCLNREQPRGRRCSNYHVR
FRCPLEASWGAWGPWGPCSGSCGPGRRLRRRHCPSPAGDACPGRPLEAQKCVRPRCPGCSLDTCECPDHI
LLGSVVTPSGQPLLGARVSLRDQPGTVATSDAHGTFRVPGVCADSRANIRAQMDGFSAGEAQAQANGSIS
VVTIILDKLEKPYLVKHPESRVREAGQNVTFCCKASGTPMPKKYSWFHNGTLLDRRAHGYGAHLELRGLR
PDQAGIYHCKAWNEAGAVRSGTARLTVLAPGQPACDPRPREYLIKLPEDCGQPGSGPAYLDVGLCPDTRC
PSLAGSSPRCGDASSRCCSVRRLERREIHCPGYVLPVKVVAECGCQKCLPPRGLVRGRVVAADSGEPLRF
ARILLGQEPIGFTAYQGDFTIEVPPSTQRLVVTFVDPSGEFMDAVRVLPFDPRGAGVYHEVKAMRKKAPV
ILHTSQSNTIPLGELEDEAPLGELVLPSGAFRRADGKPYSGPVEARVTFVDPRDLTSAASAPSDLRFVDS
DGELAPLRTYGMFSVDLRAPGSAEQLQVGPVAVRVAASQIHMPGHVEALKLWSLNPETGLWEEESGFRRE
GSSGPRVRREERVFLVGNVEIRERRLFNLDVPERRRCFVKVRAYANDKFTPSEQVEGVVVTLVNLEPGPG
FSANPRAWGRFDSAVTGPNGACLPAFCDADRPDAYTALVTATLGGEELEPAPSLPRPLPATVGVTQPYLD
RLGYRRTDHDDPAFKRNGFRINLAKPRPGDPAEANGPVYPWRSLRECQGAPVTASHFRFARVEADKYEYN
VVPFREGTPASWTGDLLAWWPNPQEFRACFLKVKIQGPQEYMVRSHNAGGSHPRTRGQLYGLRDARSVRD
PERPGTSAACVEFKCSGMLFDQRQVDRTLVTIMPQGSCRRVAVNGLLRDYLTRHPPPVPAEDPAAFSMLA
PLDPLGHNYGVYTVTDQSPRLAKEIAIGRCFDGSSDGFSREMKADAGTAVTFQCREPPAGRPSLFQRLLE
SPATALGDIRREMSEAAQAQARASGPLRTRRGRVRQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_153221
ORF Size 3468 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153221.1, NP_694953.1
RefSeq Size 3602 bp
RefSeq ORF 3471 bp
Locus ID 148113
UniProt ID Q8IUL8
Cytogenetics 19p13.11
Summary May play a role in cartilage scaffolding.[UniProtKB/Swiss-Prot Function]
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.