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PGBD1 (NM_032507) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG223042
PGBD1 (tGFP-tagged) - Human piggyBac transposable element derived 1 (PGBD1), transcript variant 2
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$789.00 $941.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PGBD1
Synonyms dJ874C20.4; HUCEP-4; SCAND4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG223042 representing NM_032507
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTATGAAGCTTTGCCAGGCCCTGCTCCTGAAAATGAAGATGGCCTTGTGAAAGTGAAGGAGGAAGATC
CCACCTGGGAGCAGGTGTGCAACTCACAGGAGGGCAGCTCCCACACTCAGGAGATTTGCCGCCTGCGCTT
TCGGCACTTCTGCTACCAGGAGGCTCACGGACCCCAGGAAGCTCTGGCCCAACTCCGAGAACTTTGTCAT
CAATGGCTGAGACCGGAGATGCACACCAAGGAACAGATAATGGAACTGCTGGTGCTGGAGCAGTTCCTGA
CCATCCTGCCCAAGGAGCTCCAGCCCTGTGTGAAGACATATCCTCTGGAGAGTGGAGAGGAGGCAGTGAC
AGTGCTGGAGAATCTAGAGACAGGAAGTGGAGACACAGGACAACAGGCCTCTGTCTATATTCAGGGACAG
GACATGCACCCAATGGTGGCAGAATATCAAGGAGTCTCTTTGGAGTGTCAGAGCCTCCAGCTCCTGCCTG
GGATAACCACCCTGAAGTGTGAACCTCCACAGCGTCCTCAAGGGAACCCCCAAGAAGTGAGTGGGCCTGT
TCCCCACGGATCAGCTCATCTCCAGGAAAAAAACCCCAGAGACAAGGCTGTAGTGCCTGTGTTTAACCCA
GTCAGGTCCCAGACATTGGTGAAGACTGAGGAAGAAACAGCCCAGGCCGTTGCTGCAGAGAAGTGGTCAC
ATCTGAGTCTGACTCGGAGGAACCTCTGTGGGAACTCAGCTCAGGAGACAGTTATGAGCCTCAGTCCGAT
GACTGAAGAAATTGTAACTAAAGATAGATTGTTTAAAGCAAAGCAAGAAACTTCTGAAGAAATGGAACAA
AGTGGAGAAGCCTCAGGAAAGCCCAACAGAGAGTGTGCACCCCAGATTCCTTGTAGTACTCCTATTGCTA
CTGAAAGGACAGTTGCACATTTGAACACTCTGAAGGACCGTCACCCAGGTGATTTGTGGGCCCGCATGCA
CATCTCATCCCTGGAATATGCTGCAGGAGACATTACCCGAAAAGGGAGAAAAAAAGACAAAGCTCGAGTG
AGTGAACTGCTCCAAGGCCTCTCATTCTCTGGTGACTCAGATGTGGAAAAAGATAATGAGCCTGAGATCC
AGCCTGCTCAAAAGAAGTTAAAGGTATCATGTTTCCCAGAAAAGAGTTGGACCAAAAGAGACATTAAACC
CAATTTTCCAAGCTGGTCAGCACTGGATTCTGGACTTTTGAATCTCAAGAGCGAAAAGTTGAACCCAGTA
GAGCTTTTTGAATTATTTTTTGATGATGAAACATTCAACTTAATTGTCAATGAAACCAATAATTATGCTT
CTCAGAAAAATGTCAGCTTGGAAGTCACAGTTCAGGAAATGAGGTGTGTGTTTGGTGTCTTACTTTTGAG
TGGATTTATGAGGCATCCTAGAAGGGAAATGTATTGGGAAGTCTCTGACACCGATCAGAACCTGGTTAGA
GATGCAATCAGAAGGGACAGATTTGAATTGATTTTCTCAAACCTGCACTTTGCAGATAATGGCCACCTAG
ATCAAAAAGATAAGTTTACAAAGTTGAGACCTCTCATAAAACAAATGAATAAAAATTTCCTCTTGTATGC
TCCCCTGGAAGAATACTATTGCTTTGATAAGTCAATGTGTGAATGCTTTGATAGTGACCAATTCCTGAAT
GGAAAGCCTATTAGAATTGGCTATAAAATTTGGTGTGGTACAACCACACAGGGTTATCTGGTTTGGTTTG
AACCCTATCAAGAAGAATCAACTATGAAGGTAGATGAGGATCCTGATCTTGGGTTAGGTGGAAATCTAGT
GATGAACTTCGCTGATGTTCTTTTAGAGAGAGGTCAGTATCCCTATCACCTGTGTTTTGATAGCTTCTTT
ACAAGTGTCAAATTGTTGTCAGCCTTGAAAAAGAAGGGGGTGAGGGCAACAGGAACAATTCGTGAGAACA
GGACCGAAAAATGTCCCCTTATGAATGTAGAACATATGAAAAAAATGAAGAGAGGGTATTTTGATTTCCG
AATAGAAGAAAACAATGAGATAATTTTGTGTCGTTGGTATGGGGATGGCATTATCAGTCTGTGCTCCAAT
GCTGTGGGCATAGAACCAGTCAATGAGGTAAGCTGTTGTGATGCTGATAACGAAGAAATCCCTCAGATAA
GTCAACCATCCATAGTAAAAGTGTATGATGAATGCAAGGAAGGTGTAGCTAAAATGGATCAAATTATTTC
GAAATACAGGGTGAGGATAAGAAGCAAGAAATGGTACTCAATTTTGGTGAGCTACATGATTGATGTAGCC
ATGAACAATGCATGGCAACTACACAGAGCCTGTAACCCAGGTGCTTCTCTAGACCCCTTGGATTTTCGGA
GATTTGTTGCACATTTCTACTTGGAACACAATGCTCATCTGTCAGAT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG223042 representing NM_032507
Red=Cloning site Green=Tags(s)

MYEALPGPAPENEDGLVKVKEEDPTWEQVCNSQEGSSHTQEICRLRFRHFCYQEAHGPQEALAQLRELCH
QWLRPEMHTKEQIMELLVLEQFLTILPKELQPCVKTYPLESGEEAVTVLENLETGSGDTGQQASVYIQGQ
DMHPMVAEYQGVSLECQSLQLLPGITTLKCEPPQRPQGNPQEVSGPVPHGSAHLQEKNPRDKAVVPVFNP
VRSQTLVKTEEETAQAVAAEKWSHLSLTRRNLCGNSAQETVMSLSPMTEEIVTKDRLFKAKQETSEEMEQ
SGEASGKPNRECAPQIPCSTPIATERTVAHLNTLKDRHPGDLWARMHISSLEYAAGDITRKGRKKDKARV
SELLQGLSFSGDSDVEKDNEPEIQPAQKKLKVSCFPEKSWTKRDIKPNFPSWSALDSGLLNLKSEKLNPV
ELFELFFDDETFNLIVNETNNYASQKNVSLEVTVQEMRCVFGVLLLSGFMRHPRREMYWEVSDTDQNLVR
DAIRRDRFELIFSNLHFADNGHLDQKDKFTKLRPLIKQMNKNFLLYAPLEEYYCFDKSMCECFDSDQFLN
GKPIRIGYKIWCGTTTQGYLVWFEPYQEESTMKVDEDPDLGLGGNLVMNFADVLLERGQYPYHLCFDSFF
TSVKLLSALKKKGVRATGTIRENRTEKCPLMNVEHMKKMKRGYFDFRIEENNEIILCRWYGDGIISLCSN
AVGIEPVNEVSCCDADNEEIPQISQPSIVKVYDECKEGVAKMDQIISKYRVRIRSKKWYSILVSYMIDVA
MNNAWQLHRACNPGASLDPLDFRRFVAHFYLEHNAHLSD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032507
ORF Size 2427 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032507.3, NP_115896.1
RefSeq Size 3082 bp
RefSeq ORF 2430 bp
Locus ID 84547
UniProt ID Q96JS3
Cytogenetics 6p22.1
Domains LER
Protein Families Druggable Genome, Transcription Factors
Summary The piggyBac family of proteins, found in diverse animals, are transposases related to the transposase of the canonical piggyBac transposon from the moth, Trichoplusia ni. This family also includes genes in several genomes, including human, that appear to have been derived from the piggyBac transposons. This gene belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel, with no obvious relationship to other transposases, or other known protein families. This gene product is specifically expressed in the brain, however, its exact function is not known. Alternative splicing results in multiple transcript variants encoding the same protein.provided by RefSeq, May 2010
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Documents
Resources
"NM_032507" in other vectors (4)
SKU Description Size Price
RC223042 PGBD1 (Myc-DDK-tagged)-Human piggyBac transposable element derived 1 (PGBD1), transcript variant 2 10 ug
$741.00
RC223042L3 Lenti ORF clone of Human piggyBac transposable element derived 1 (PGBD1), transcript variant 2, Myc-DDK-tagged 10 ug
$1,041.00
RC223042L4 Lenti ORF clone of Human piggyBac transposable element derived 1 (PGBD1), transcript variant 2, mGFP tagged 10 ug
$1,041.00
SC314746 PGBD1 (untagged)-Human piggyBac transposable element derived 1 (PGBD1), transcript variant 2 10 ug
$816.00

Plasmid Map for RG223042

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG223042
Circular map for RG223042

file-alt Citations

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