ZDHHC19 (NM_001039617) Human Tagged ORF Clone

  • TrueORF®
SKU
RG221741
ZDHHC19 (tGFP-tagged) - Human zinc finger, DHHC-type containing 19 (ZDHHC19)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZDHHC19
Synonyms DHHC19
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG221741 representing NM_001039617
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACACTCTTAACGGATGCCACGCCGCTGGTGAAGGAGCCCCATCCCCTGCCTCTGGTCCCACGTCCCT
GGTTCCTCCCTAGCCTCTTTGCTGCCTTCAATGTGGTGCTGCTGGTCTTTTTCAGTGGCCTCTTCTTCGC
ATTCCCTTGCAGGTGGCTGGCTCAGAACGGGGAGTGGGCCTTTCCTGTTATCACAGGCTCCCTCTTTGTC
CTTACCTTCTTCAGTCTTGTTTCACTCAACTTCTCAGACCCTGGCATCTTACATCAAGGCTCCGCTGAGC
AGGGCCCCTTGACGGTGCACGTGGTGTGGGTGAACCACGGGGCCTTCCGCCTGCAATGGTGTCCAAAGTG
CTGCTTCCACCGCCCGCCCCGGACTTACCACTGCCCCTGGTGCAACATCTGTGTGGAGGACTTTGACCAC
CACTGCAAGTGGGTCAATAACTGCATCGGTCACCGCAACTTCCGCTTCTTCATGCTGCTTGTCCTGTCCC
TGTGCCTCTACTCGGGCGCCATGCTGGTCACCTGTCTCATCTTCCTGGTGCGCACAACCCACCTGCCCTT
CTCCACCGACAAGGCCATCGCCATCGTGGTGGCCGTGTCCGCCGCGGGCCTCCTGGTGCCGCTGTCCCTC
CTGCTGCTGATCCAGGCACTGTCCGTGAGCTCGGCCGACCGCACCTACAAGGGCAAGTGCAGACACCTTC
AGGGATACAACCCCTTCGACCAGGGCTGTGCCAGCAACTGGTATTTAACAATTTGTGCACCACTGGGACC
CAAGTACATGGCTGAAGCTGTCCAGCTGCAGAGAGTGGTGGGGCCTGACTGGACATCCATGCCGAATCTG
CACCCTCCAATGTCCCCCTCTGCTCTCAACCCCCCAGCCCCAACCTCTGGGTCCCTACAAAGCAGGGAAG
GGACCCCCGGGGCGTGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG221741 representing NM_001039617
Red=Cloning site Green=Tags(s)

MTLLTDATPLVKEPHPLPLVPRPWFLPSLFAAFNVVLLVFFSGLFFAFPCRWLAQNGEWAFPVITGSLFV
LTFFSLVSLNFSDPGILHQGSAEQGPLTVHVVWVNHGAFRLQWCPKCCFHRPPRTYHCPWCNICVEDFDH
HCKWVNNCIGHRNFRFFMLLVLSLCLYSGAMLVTCLIFLVRTTHLPFSTDKAIAIVVAVSAAGLLVPLSL
LLLIQALSVSSADRTYKGKCRHLQGYNPFDQGCASNWYLTICAPLGPKYMAEAVQLQRVVGPDWTSMPNL
HPPMSPSALNPPAPTSGSLQSREGTPGAW

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001039617
ORF Size 927 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039617.2
RefSeq Size 1370 bp
RefSeq ORF 930 bp
Locus ID 131540
UniProt ID Q8WVZ1
Cytogenetics 3q29
Protein Families Transmembrane
Summary Palmitoyltransferase that mediates palmitoylation of STAT3 and RRAS (PubMed:31462771, PubMed:20074548). Palmitoylation of STAT3 induces the homodimerization and transcriptional activation of STAT3, thereby, promoting inflammation and cancer (PubMed:31462771). Palmitoylation of RRAS is linked to cell viability (PubMed:20074548). May be involved in S-stearoylation of STAT3 (Probable).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001039617" in other vectors (4)
SKU Description Size Price
RC221741 ZDHHC19 (Myc-DDK-tagged)-Human zinc finger, DHHC-type containing 19 (ZDHHC19) 10 ug
$300.00
RC221741L3 Lenti ORF clone of Human zinc finger, DHHC-type containing 19 (ZDHHC19), Myc-DDK-tagged 10 ug
$600.00
RC221741L4 Lenti ORF clone of Human zinc finger, DHHC-type containing 19 (ZDHHC19), mGFP tagged 10 ug
$600.00
SC310746 ZDHHC19 (untagged)-Human zinc finger, DHHC-type containing 19 (ZDHHC19) 10 ug
$330.00

Plasmid Map for RG221741

Download sequence for

Cloning scheme for RG221741

Circular map for RG221741

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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