KDM5A (NM_001042603) Human Tagged ORF Clone

  • TrueORF®
SKU
RG221461
KDM5A (tGFP-tagged) - Human lysine (K)-specific demethylase 5A (KDM5A)
  $2,330.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KDM5A
Synonyms RBBP-2; RBBP2; RBP2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001042603
ORF Size 5070 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001042603.3
RefSeq Size 10835 bp
RefSeq ORF 5073 bp
Locus ID 5927
UniProt ID P29375
Cytogenetics 12p13.33
Protein Families Druggable Genome
Summary This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression. provided by RefSeq, Aug 2013
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Documents
Resources
"NM_001042603" in other vectors (4)
SKU Description Size Price
RC221461 KDM5A (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 5A (KDM5A) 10 ug
$2,130.00
RC221461L1 Lenti ORF clone of Human lysine (K)-specific demethylase 5A (KDM5A), Myc-DDK-tagged 10 ug
$2,430.00
RC221461L3 Lenti ORF clone of Human lysine (K)-specific demethylase 5A (KDM5A), Myc-DDK-tagged 10 ug
$2,430.00
SC311431 KDM5A (untagged)-Human lysine (K)-specific demethylase 5A (KDM5A) 10 ug
$2,131.00

Plasmid Map for RG221461

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Cloning scheme for RG221461

Circular map for RG221461

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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