ABCC12 (NM_033226) Human Tagged ORF Clone

SKU RG221421

ABCC12 (tGFP-tagged) - Human ATP-binding cassette, sub-family C (CFTR/MRP), member 12 (ABCC12)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ABCC12
• Synonyms: MRP9
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221421 representing NM_033226; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGTGGGTGAAGGACCCTACCTTATCTCAGATCTGGACCAGCGAGGCCGGCGGAGATCCTTTGCAGAAA; GATATGACCCCAGCCTGAAGACCATGATCCCAGTGCGACCCTGTGCAAGGTTAGCACCCAACCCGGTGGA; TGATGCCGGGCTACTCTCCTTCGCCACATTTTCCTGGCTCACGCCGGTGATGGTGAAAGGCTACCGGCAA; AGGCTGACCGTAGACACCCTGCCCCCATTGTCGACATATGACTCATCTGACACCAATGCCAAAAGATTTC; GAGTCCTTTGGGATGAAGAGGTAGCAAGGGTGGGTCCTGAGAAGGCCTCTCTGAGCCACGTGGTGTGGAA; ATTCCAGAGGACACGCGTGTTGATGGACATCGTGGCCAACATCCTGTGCATCATCATGGCAGCCATAGGG; CCGGTGATTCTCATTCACCAAATCCTCCAGCAGACTGAGAGGACCTCTGGGAAAGTCTGGGTTGGCATTG; GACTGTGCATAGCCCTTTTTGCCACCGAGTTTACCAAAGTCTTCTTTTGGGCCCTTGCCTGGGCCATCAA; CTACCGCACGGCCATCCGGTTGAAGGTGGCGCTCTCCACCTTGGTTTTTGAAAACCTAGTGTCCTTCAAG; ACATTGACCCACATCTCTGTTGGCGAGGTGCTCAATATACTGTCAAGTGATAGCTATTCTTTGTTTGAAG; CTGCCTTGTTTTGTCCTTTGCCAGCCACCATCCCGATCCTAATGGTCTTTTGTGCGGCGTACGCCTTTTT; CATTCTGGGGCCCACAGCTCTCATCGGGATATCAGTGTATGTCATATTCATACCCGTCCAGATGTTTATG; GCCAAGCTCAATTCAGCTTTCCGAAGGTCAGCAATTTTGGTGACAGACAAGCGAGTTCAGACAATGAATG; AGTTTCTGACCTGCATCAGGCTGATCAAAATGTATGCCTGGGAGAAATCTTTTACCAACACTATCCAAGA; TATAAGAAGGAGGGAAAGAAAATTACTGGAAAAAGCTGGATTTGTCCAAAGTGGAAACTCTGCCCTGGCC; CCCATCGTGTCCACCATAGCCATCGTGCTGACATTATCCTGCCACATCCTCCTGAGACGCAAACTCACCG; CACCCGTGGCATTTAGTGTGATTGCCATGTTTAATGTAATGAAGTTTTCCATTGCAATCTTGCCCTTCTC; CATCAAAGCAATGGCTGAAGCGAATGTCTCTCTAAGGAGAATGAAGAAAATTCTCATAGATAAAAGCCCC; CCATCTTACATCACCCAACCAGAAGACCCAGATACTGTCTTGCTTTTAGCAAATGCCACCTTGACATGGG; AGCATGAAGCCAGCAGGAAAAGTACCCCAAAGAAATTGCAGAACCAGAAAAGGCATTTATGCAAGAAACA; GAGGTCAGAGGCATACAGTGAGAGGAGTCCACCAGCCAAGGGAGCCACTGGCCCAGAGGAGCAAAGTGAC; AGCCTCAAATCGGTTCTGCACAGCATAAGCTTTGTGGTGAGAAAGGGGAAGATCTTGGGAATATGTGGGA; ATGTGGGAAGTGGAAAGAGCTCCCTCCTTGCAGCTCTCCTAGGACAGATGCAGCTGCAGAAAGGGGTGGT; GGCAGTCAATGGAACTTTGGCCTACGTTTCACAGCAGGCATGGATCTTTCATGGAAATGTGAGAGAAAAC; ATACTCTTTGGAGAAAAGTATGATCACCAAAGGTATCAGCACACAGTCCGCGTCTGTGGCCTCCAGAAGG; ACCTGAGCAACCTCCCCTATGGAGACCTGACTGAGATTGGGGAGCGGGGCCTCAACCTCTCTGGGGGGCA; GAGGCAGAGGATTAGCCTGGCCCGCGCTGTCTACTCCGACCGTCAGCTCTACCTGCTGGACGACCCCCTG; TCGGCCGTGGACGCCCACGTGGGGAAGCACGTCTTTGAGGAGTGCATTAAGAAGACGCTCAGGGGAAAGA; CAGTCGTCCTGGTGACCCACCAGCTACAGTTCTTAGAGTCTTGTGATGAAGTTATTTTATTAGAAGATGG; AGAGATTTGTGAAAAGGGAACCCACAAGGAGTTAATGGAGGAGAGAGGGCGCTATGCAAAACTGATTCAC; AACCTGCGAGGATTGCAGTTCAAGGATCCTGAACACCTTTACAATGCAGCAATGGTGGAAGCCTTCAAGG; AGAGCCCTGCTGAGAGAGAGGAAGATGCTGGTATAATCGTTTTGGCTCCAGGAAATGAGAAAGATGAAGG; AAAAGAATCTGAAACAGGCTCAGAATTTGTAGACACAAAAGTTCCTGAGCACCAGCTCATCCAGACTGAA; TCCCCCCAGGAAGGAACCGTGACCTGGAAAACATATCACACGTACATTAAGGCTTCTGGAGGGTACCTCC; TTTCTCTCTTCACTGTGTTCCTCTTCCTCCTGATGATTGGCAGCGCTGCCTTCAGCAACTGGTGGCTGGG; TCTCTGGTTGGACAAGGGCTCACGGATGACCTGTGGGCCCCAGGGCAACAGGACCATGTGTGAGGTCGGC; GCGGTGCTGGCAGACATCGGTCAGCATGTGTACCAGTGGGTGTACACTGCAAGCATGGTGTTCATGCTGG; TGTTTGGCGTCACCAAAGGCTTCGTCTTCACCAAGACCACACTGATGGCATCCTCCTCTCTGCATGACAC; GGTGTTTGATAAGATCTTAAAGAGCCCAATGAGTTTCTTTGACACGACTCCCACTGGCAGGCTAATGAAC; CGTTTTTCCAAGGATATGGACGAGCTGGATGTGAGGCTGCCGTTTCACGCAGAGAACTTTCTGCAGCAGT; TTTTTATGGTGGTGTTTATTCTCGTGATCTTGGCTGCTGTGTTTCCTGCTGTCCTTTTAGTCGTGGCCAG; CCTTGCTGTAGGCTTCTTCATTCTGTTACGCATTTTCCACAGAGGAGTCCAGGAGCTCAAGAAGGTGGAG; AATGTCAGCCGGTCACCCTGGTTCACCCACATCACCTCCTCCATGCAGGGCCTGGGCATCATTCACGCCT; ATGGCAAGAAGGAGAGCTGCATCACCTATCACCTCCTCTACTTTAACTGTGCTCTCAGGTGGTTTGCGCT; GAGAATGGATGTCCTCATGAACATCCTTACCTTCACTGTGGCCTTGTTGGTGACCCTGAGTTTCTCCTCC; ATCAGTACTTCATCCAAAGGCCTGTCATTGTCATACATCATCCAGCTGAGCGGACTGCTCCAAGTGTGTG; TGCGAACGGGAACAGAGACGCAAGCCAAATTCACCTCCGTGGAGCTGCTCAGGGAATACATTTCGACCTG; TGTTCCTGAATGCACTCATCCCCTCAAAGTGGGGACCTGTCCCAAGGACTGGCCCAGCTGTGGGGAGATC; ACCTTCAGAGACTATCAGATGAGATACAGAGACAACACCCCCCTTGTTCTCGACAGCCTGAACTTGAACA; TACAAAGTGGGCAGACAGTCGGGATTGTTGGAAGAACAGGTTCCGGAAAGTCATCGTTAGGAATGGCTTT; GTTTCGTCTGGTGGAGCCAGCCAGTGGCACAATCTTTATTGATGAGGTGGATATCTGCATTCTCAGCTTG; GAAGACCTCAGAACCAAGCTGACTGTGATCCCACAGGATCCTGTCCTGTTTGTAGGTACAGTAAGGTACA; ACTTGGATCCCTTTGAGAGTCACACCGATGAGATGCTCTGGCAGGTTCTGGAGAGAACATTCATGAGAGA; CACAATAATGAAACTCCCAGAAAAATTACAGGCAGAAGTCACAGAAAATGGAGAAAACTTCTCAGTAGGG; GAACGTCAGCTGCTTTGTGTGGCCCGAGCTCTTCTCCGTAATTCAAAGATCATTCTCCTTGATGAAGCCA; CCGCCTCTATGGACTCCAAGACTGACACCCTGGTTCAGAACACCATCAAAGATGCCTTCAAGGGCTGCAC; TGTGCTGACCATCGCCCACCGCCTCAACACAGTTCTCAACTGCGATCACGTCCTGGTTATGGAAAATGGG; AAGGTGATTGAGTTTGACAAGCCTGAAGTCCTTGCAGAGAAGCCAGATTCTGCATTTGCGATGTTACTAG; CAGCAGAAGTCAGATTG; ; AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221421 representing NM_033226; Red=Cloning site Green=Tags(s); ; MVGEGPYLISDLDQRGRRRSFAERYDPSLKTMIPVRPCARLAPNPVDDAGLLSFATFSWLTPVMVKGYRQ; RLTVDTLPPLSTYDSSDTNAKRFRVLWDEEVARVGPEKASLSHVVWKFQRTRVLMDIVANILCIIMAAIG; PVILIHQILQQTERTSGKVWVGIGLCIALFATEFTKVFFWALAWAINYRTAIRLKVALSTLVFENLVSFK; TLTHISVGEVLNILSSDSYSLFEAALFCPLPATIPILMVFCAAYAFFILGPTALIGISVYVIFIPVQMFM; AKLNSAFRRSAILVTDKRVQTMNEFLTCIRLIKMYAWEKSFTNTIQDIRRRERKLLEKAGFVQSGNSALA; PIVSTIAIVLTLSCHILLRRKLTAPVAFSVIAMFNVMKFSIAILPFSIKAMAEANVSLRRMKKILIDKSP; PSYITQPEDPDTVLLLANATLTWEHEASRKSTPKKLQNQKRHLCKKQRSEAYSERSPPAKGATGPEEQSD; SLKSVLHSISFVVRKGKILGICGNVGSGKSSLLAALLGQMQLQKGVVAVNGTLAYVSQQAWIFHGNVREN; ILFGEKYDHQRYQHTVRVCGLQKDLSNLPYGDLTEIGERGLNLSGGQRQRISLARAVYSDRQLYLLDDPL; SAVDAHVGKHVFEECIKKTLRGKTVVLVTHQLQFLESCDEVILLEDGEICEKGTHKELMEERGRYAKLIH; NLRGLQFKDPEHLYNAAMVEAFKESPAEREEDAGIIVLAPGNEKDEGKESETGSEFVDTKVPEHQLIQTE; SPQEGTVTWKTYHTYIKASGGYLLSLFTVFLFLLMIGSAAFSNWWLGLWLDKGSRMTCGPQGNRTMCEVG; AVLADIGQHVYQWVYTASMVFMLVFGVTKGFVFTKTTLMASSSLHDTVFDKILKSPMSFFDTTPTGRLMN; RFSKDMDELDVRLPFHAENFLQQFFMVVFILVILAAVFPAVLLVVASLAVGFFILLRIFHRGVQELKKVE; NVSRSPWFTHITSSMQGLGIIHAYGKKESCITYHLLYFNCALRWFALRMDVLMNILTFTVALLVTLSFSS; ISTSSKGLSLSYIIQLSGLLQVCVRTGTETQAKFTSVELLREYISTCVPECTHPLKVGTCPKDWPSCGEI; TFRDYQMRYRDNTPLVLDSLNLNIQSGQTVGIVGRTGSGKSSLGMALFRLVEPASGTIFIDEVDICILSL; EDLRTKLTVIPQDPVLFVGTVRYNLDPFESHTDEMLWQVLERTFMRDTIMKLPEKLQAEVTENGENFSVG; ERQLLCVARALLRNSKIILLDEATASMDSKTDTLVQNTIKDAFKGCTVLTIAHRLNTVLNCDHVLVMENG; KVIEFDKPEVLAEKPDSAFAMLLAAEVRL; ; SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033226
• ORF Size: 4077 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_033226.1, NP_150229.1
• RefSeq Size: 5168 bp
• RefSeq ORF: 4080 bp
• Locus ID: 94160
• UniProt ID: Q96J65
• Cytogenetics: 16q12.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• Summary: This gene is a member of the superfamily of ATP-binding cassette (ABC) transporters and the encoded protein contains two ATP-binding domains and 12 transmembrane regions. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies: ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White. This gene is a member of the MRP subfamily which is involved in multi-drug resistance. This gene and another subfamily member are arranged head-to-tail on chromosome 16q12.1. Increased expression of this gene is associated with breast cancer. [provided by RefSeq, Jul 2008]