PKN2 (NM_006256) Human Tagged ORF Clone

SKU RG220989

PKN2 (tGFP-tagged) - Human protein kinase N2 (PKN2)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PKN2
• Synonyms: Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220989 representing NM_006256; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGTCCAACCCCGAACGGGGGGAGATTCTGCTCACGGAACTGCAGGGGGATTCCCGAAGTCTTCCGT; TTTCTGAGAATGTGAGTGCTGTTCAAAAATTAGACTTTTCAGATACAATGGTGCAGCAGAAATTGGATGA; TATCAAGGATCGAATTAAGAGAGAAATAAGGAAAGAACTGAAAATCAAAGAAGGAGCTGAAAATCTGAGG; AAAGTCACAACAGATAAAAAAAGTTTGGCTTATGTAGACAACATTTTGAAAAAATCAAATAAAAAATTAG; AAGAACTACATCACAAGCTGCAGGAATTAAATGCACATATTGTTGTATCAGATCCAGAAGATATTACAGA; TTGCCCAAGGACTCCAGATACTCCAAATAATGACCCTCGTTGTTCTACTAGCAACAATAGATTGAAGGCC; TTACAAAAACAATTGGATATAGAACTTAAAGTAAAACAAGGTGCAGAGAATATGATACAGATGTATTCAA; ATGGATCTTCAAAGGATCGGAAACTCCATGGTACAGCTCAGCAACTGCTCCAGGACAGCAAGACAAAAAT; AGAAGTCATACGAATGCAGATTCTTCAGGCAGTCCAGACTAATGAATTGGCTTTTGATAATGCAAAACCT; GTGATAAGTCCTCTTGAACTTCGGATGGAAGAATTAAGGCATCATTTTAGGATAGAGTTTGCAGTAGCAG; AAGGTGCAAAGAATGTAATGAAATTACTTGGCTCAGGAAAAGTAACAGACAGAAAAGCACTTTCAGAAGC; TCAAGCAAGATTTAATGAATCAAGTCAGAAGTTGGACCTTTTAAAGTATTCATTAGAGCAAAGATTAAAC; GAAGTCCCCAAGAATCATCCCAAAAGCAGGATTATTATTGAAGAACTTTCACTTGTTGCTGCATCACCAA; CACTAAGTCCACGTCAAAGTATGATATCTACGCAAAATCAATATAGTACACTATCCAAACCAGCAGCACT; AACAGGTACTTTGGAAGTTCGTCTTATGGGCTGCCAAGATATCCTAGAGAATGTCCCTGGACGGTCAAAA; GCAACATCAGTTGCACTGCCTGGTTGGAGTCCAAGTGAAACCAGATCATCTTTCATGAGCAGAACGAGTA; AAAGTAAAAGCGGAAGTAGTCGAAATCTTCTAAAAACCGATGACTTGTCCAATGATGTCTGTGCTGTTTT; GAAGCTCGATAATACTGTGGTTGGCCAAACTAGCTGGAAACCCATTTCCAATCAGTCATGGGACCAGAAG; TTTACACTGGAACTGGACAGGTCACGTGAACTGGAAATTTCAGTTTATTGGCGTGATTGGCGGTCTCTGT; GTGCTGTAAAATTTCTGAGGTTAGAAGATTTTTTAGACAACCAACGGCATGGCATGTGTCTCTATTTGGA; ACCACAGGGTACTTTATTTGCAGAGGTTACCTTTTTTAATCCAGTTATTGAAAGAAGACCAAAACTTCAA; AGACAAAAGAAAATTTTTTCAAAGCAACAAGGCAAAACATTTCTCAGAGCTCCTCAAATGAATATTAATA; TTGCCACTTGGGGAAGGCTAGTAAGAAGAGCTATTCCTACAGTAAATCATTCTGGCACCTTCAGCCCTCA; AGCTCCTGTGCCTACTACAGTGCCAGTGGTTGATGTACGCATCCCTCAACTAGCACCTCCAGCTAGTGAT; TCTACAGTAACCAAATTGGACTTTGATCTTGAGCCTGAACCTCCTCCAGCCCCACCACGAGCTTCTTCTC; TTGGAGAAATAGATGAATCTTCTGAATTAAGAGTTTTGGATATACCAGGACAGGATTCAGAGACTGTTTT; TGATATTCAGAATGACAGAAATAGTATACTTCCAAAATCTCAATCTGAATACAAGCCTGATACTCCTCAG; TCAGGCCTAGAATATAGTGGTATTCAAGAACTTGAGGACAGAAGATCTCAGCAAAGGTTTCAGTTTAATC; TACAAGATTTCAGGTGTTGTGCTGTCTTGGGAAGAGGACATTTTGGAAAGGTGCTTTTAGCTGAATATAA; AAACACAAATGAGATGTTTGCTATAAAAGCCTTAAAGAAAGGAGATATTGTGGCTCGAGATGAAGTAGAC; AGCCTGATGTGTGAAAAAAGAATTTTTGAAACTGTGAATAGTGTAAGGCATCCCTTTTTGGTGAACCTTT; TTGCATGTTTCCAAACCAAAGAGCATGTTTGCTTTGTAATGGAATATGCTGCCGGTGGGGACCTAATGAT; GCACATTCATACTGATGTCTTTTCTGAACCAAGAGCTGTATTTTATGCTGCTTGTGTAGTTCTTGGGTTG; CAGTATTTACATGAACACAAAATTGTTTATAGAGATTTGAAATTGGATAACTTATTGCTAGATACAGAGG; GCTTTGTGAAAATTGCTGATTTTGGTCTTTGCAAAGAAGGAATGGGATATGGAGATAGAACAAGCACATT; TTGTGGCACTCCTGAATTTCTTGCCCCAGAAGTATTAACAGAAACTTCTTATACAAGGGCTGTAGATTGG; TGGGGCCTTGGCGTGCTTATATATGAAATGCTTGTTGGTGAGTCTCCCTTTCCTGGTGATGATGAAGAGG; AAGTTTTTGACAGTATTGTAAATGATGAAGTAAGGTATCCAAGGTTCTTATCTACAGAAGCCATTTCTAT; AATGAGAAGGCTGTTAAGAAGAAATCCTGAACGGCGCCTTGGGGCTAGCGAGAAAGATGCAGAGGATGTA; AAAAAGCACCCATTTTTCCGGCTAATTGATTGGAGCGCTCTGATGGACAAAAAAGTAAAGCCACCATTTA; TACCTACCATAAGAGGACGAGAAGATGTTAGTAATTTTGATGATGAATTTACCTCAGAAGCACCTATTCT; GACTCCACCTCGAGAACCAAGGATACTTTCGGAAGAGGAGCAGGAAATGTTCAGAGATTTTGACTACATT; GCTGATTGGTGT; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220989 representing NM_006256; Red=Cloning site Green=Tags(s); ; MASNPERGEILLTELQGDSRSLPFSENVSAVQKLDFSDTMVQQKLDDIKDRIKREIRKELKIKEGAENLR; KVTTDKKSLAYVDNILKKSNKKLEELHHKLQELNAHIVVSDPEDITDCPRTPDTPNNDPRCSTSNNRLKA; LQKQLDIELKVKQGAENMIQMYSNGSSKDRKLHGTAQQLLQDSKTKIEVIRMQILQAVQTNELAFDNAKP; VISPLELRMEELRHHFRIEFAVAEGAKNVMKLLGSGKVTDRKALSEAQARFNESSQKLDLLKYSLEQRLN; EVPKNHPKSRIIIEELSLVAASPTLSPRQSMISTQNQYSTLSKPAALTGTLEVRLMGCQDILENVPGRSK; ATSVALPGWSPSETRSSFMSRTSKSKSGSSRNLLKTDDLSNDVCAVLKLDNTVVGQTSWKPISNQSWDQK; FTLELDRSRELEISVYWRDWRSLCAVKFLRLEDFLDNQRHGMCLYLEPQGTLFAEVTFFNPVIERRPKLQ; RQKKIFSKQQGKTFLRAPQMNINIATWGRLVRRAIPTVNHSGTFSPQAPVPTTVPVVDVRIPQLAPPASD; STVTKLDFDLEPEPPPAPPRASSLGEIDESSELRVLDIPGQDSETVFDIQNDRNSILPKSQSEYKPDTPQ; SGLEYSGIQELEDRRSQQRFQFNLQDFRCCAVLGRGHFGKVLLAEYKNTNEMFAIKALKKGDIVARDEVD; SLMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFVMEYAAGGDLMMHIHTDVFSEPRAVFYAACVVLGL; QYLHEHKIVYRDLKLDNLLLDTEGFVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDW; WGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGASEKDAEDV; KKHPFFRLIDWSALMDKKVKPPFIPTIRGREDVSNFDDEFTSEAPILTPPREPRILSEEEQEMFRDFDYI; ADWC; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006256
• ORF Size: 2952 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006256.4
• RefSeq Size: 3255 bp
• RefSeq ORF: 2955 bp
• Locus ID: 5586
• UniProt ID: Q16513
• Cytogenetics: 1p22.2
• Domains: C2, HR1, pkinase, S_TKc, S_TK_X, TyrKc
• Protein Families: Druggable Genome, Protein Kinase
• Summary: PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Involved in the negative regulation of ciliogenesis (PubMed:27104747).[UniProtKB/Swiss-Prot Function]