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RSPH10B (NM_173565) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG220976
RSPH10B (tGFP-tagged) - Human radial spoke head 10 homolog B (Chlamydomonas) (RSPH10B)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$789.00 $997.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RSPH10B
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG220976 representing NM_173565
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGAAAGAAAAGAAAAAAGCAGACAAAAAAGGGGAGAAGTCTGCCCGCTCTCCCTCATCTCTCTCTG
ATAATCTAGACTTTTCCAAACAAGATGGCAACACCACTAGGCAAGAGATGTCCCCAGCTGGTGTCCCATT
GCTGGGAATGCAGCTCAACGAAGTGAAACCCAAAAAAGACCGCCAAAACGTTCAGCAGAACGAAGATGCC
AGCCAATACGAAGAGTCCATTCTGACCAAACTCATAGTGGAAAGCTATGAAGGGGAAAAGGTTCGTGGGC
TGTATGAGGGAGAAGGCTTCGCGGCCTTTCAAGGCGGTTGTACCTATCGTGGTATGTTTTCAGAAGGACT
CATGCATGGACAGGGGACTTATATTTGGGCCGATGGATTAAAATATGAGGGCGACTTTGTGAAGAATGTC
CCGATGAACCACGGCGTGTACACGTGGCCGGACGGCAGCATGTATGAAGGCGAAGTGGTCAACGGCATGA
GGAACGGATTCGGGATGTTCAAGTGCAGCACCCAGCCTGTGTCCTACATCGGCCACTGGTGCAATGGCAA
GCGGCACGGGAAGGGCTCCATTTATTACAATCAAGAGGGTACGTGTTGGTACGAGGGAGACTGGGTACAA
AACATCAAAAAGGGCTGGGGAATAAGATGTTATAAATCTGGAAATATATACGAAGGCCAGTGGGAAGACA
ACATGCGCCACGGGGAGGGGAGGATGAGGTGGCTGACCACCAACGAAGAGTACACCGGGCGGTGGGAGAG
GGGCATCCAGAATGGCTTTGGAACACACACATGGTTTCTAAAGAGAATCCGCAGTTCCCAGTATCCTTTG
AGAAATGAATACATAGGGGAGTTTGTAAATGGATATCGTCACGGACGTGGCAAGTTTTATTATGCCAGTG
GAGCCATGTATGATGGAGAATGGGTTTCCAATAAGAAACATGGCATGGGCCGATTAACTTTCAAGAACGG
GCGTGTGTACGAAGGCGCATTCTCCAATGACCACATAGCTGGGTTTCCGGATCTTGAAGTTGAATTCATC
AGCTGCCTGGACCTGTCTTCAGGAGTTGCCCCAAGACTGTCCAGGAGCGCCGAACTGATCAGAAAGCTTG
ATGGCAGTGAAAGTCATTCTGTGTTGGGATCGAGCATTGAGCTGGATCTAAATTTGCTCCTGGACATGTA
CCCTGAGACAGTCCAACCTGAAGAAAAGAAGCAGGTGGAATATGCCGTCTTAAGAAATATTACAGAATTA
AGAAGAATCTACAGCTTTTACAGCAGCCTGGGATGCGGCCACTCTCTGGATAATACCTTTCTGATGACAA
AGCTTCACTTCTGGAGATTTCTAAAAGATTGCAAATTTCATCACCACAAACTAACTCTTGCTGATATGGA
CAGGATATTAAGTGCCAATAATGACATACCAGTTGAAGAAATCCATTCTCCATTTACAACAATACTTTTG
AGAACATTTTTGAATTACCTCCTGCATTTGGCGTACCACATTTATCATGAAGAATTCCAAAAGAGAAGCC
CATCCCTCTTCTTGTGTTTTACAAAACTGATGACCGAGAACATTCGTCCAAATGCCTGCCAGATAAAAGG
CAATTTATTCCGTGAGCAACAGCGGACGCTCTACTCTATAAGTTACATGAATAAGTGCTGGGAGATTTAT
CTCGCTTACTGCAGACCCAGCGCAGCGCCTCCCCACGAGCCTACGATGAAGATGAGACACTTCCTCTGGA
TGCTGAAAGACTTTAAAATGATAAATAAAGAATTAACAGCAGCTACATTTATGGAGGTCATAGCAGAGGA
TAATCGTTTCATATATGATGGAATTGACAGCAACTTTGAACCTGAGCTGGTTTTCCTGGAATTCTTTGAA
GCTCTCTTAAGCTTTGCATTCATCTGTGTTACTGACCAAATGACTAAATCCTATACAAATGTTCCAGCTG
ATGATGTGTCTGGAAATAAACATGAAACTATTTATACAATACTAAATCAGGACGCCCAGAACAAGAGTCC
CAGCGCGGTCATGAGCCACGAATCGGATGCTGCTCACTCTGACAGTGCCAGGTCATCTTCCAGCAAGTTA
GAACTCTCGCCTGATGTTAACAAAATAAGGAAATCAGAGCCCAAGATCAAGAAGTCTGTAAGTCATGAAA
GAGTCTCCAAAATGAATTTTAAATTGACTGGAAAAGGGATCACCTTTTTCTCATCTGAGAGCAAGAAATA
TGAGAGACCCAAGGATGATCGAGAGGAAGAGTTCAACACGTGGGTCAATAATATGTACGTCTTCTTTGTG
AACACGCTCTTTCATGCGTATAAACGTGAAGAAGCTATCAAGGAGAAAATAAGGGCAGACAGGTTACGTA
GCACAGCACAGGCCCAGCAGCGGAAGATGGAAGATGACGAACTGGAAGCAAGGCTGAACATCTTCATCTT
GAGAGAGGAAGAGGCCAAGAGACATGACTATGAGGTGGACATCACAGTGCTCAAGAAGCCGGCAGACGTG
TCATCCTCTCACCTCATACTGGACCCTCCCAAGGAGGATGTGACCGTGTCCCCATCCAGCAAGACCATCA
CCAGCAAGAAGAAGAAAAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG220976 representing NM_173565
Red=Cloning site Green=Tags(s)

MVKEKKKADKKGEKSARSPSSLSDNLDFSKQDGNTTRQEMSPAGVPLLGMQLNEVKPKKDRQNVQQNEDA
SQYEESILTKLIVESYEGEKVRGLYEGEGFAAFQGGCTYRGMFSEGLMHGQGTYIWADGLKYEGDFVKNV
PMNHGVYTWPDGSMYEGEVVNGMRNGFGMFKCSTQPVSYIGHWCNGKRHGKGSIYYNQEGTCWYEGDWVQ
NIKKGWGIRCYKSGNIYEGQWEDNMRHGEGRMRWLTTNEEYTGRWERGIQNGFGTHTWFLKRIRSSQYPL
RNEYIGEFVNGYRHGRGKFYYASGAMYDGEWVSNKKHGMGRLTFKNGRVYEGAFSNDHIAGFPDLEVEFI
SCLDLSSGVAPRLSRSAELIRKLDGSESHSVLGSSIELDLNLLLDMYPETVQPEEKKQVEYAVLRNITEL
RRIYSFYSSLGCGHSLDNTFLMTKLHFWRFLKDCKFHHHKLTLADMDRILSANNDIPVEEIHSPFTTILL
RTFLNYLLHLAYHIYHEEFQKRSPSLFLCFTKLMTENIRPNACQIKGNLFREQQRTLYSISYMNKCWEIY
LAYCRPSAAPPHEPTMKMRHFLWMLKDFKMINKELTAATFMEVIAEDNRFIYDGIDSNFEPELVFLEFFE
ALLSFAFICVTDQMTKSYTNVPADDVSGNKHETIYTILNQDAQNKSPSAVMSHESDAAHSDSARSSSSKL
ELSPDVNKIRKSEPKIKKSVSHERVSKMNFKLTGKGITFFSSESKKYERPKDDREEEFNTWVNNMYVFFV
NTLFHAYKREEAIKEKIRADRLRSTAQAQQRKMEDDELEARLNIFILREEEAKRHDYEVDITVLKKPADV
SSSHLILDPPKEDVTVSPSSKTITSKKKKK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173565
ORF Size 2610 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173565.2
RefSeq Size 3125 bp
RefSeq ORF 2613 bp
Locus ID 222967
UniProt ID B2RC85
Cytogenetics 7p22.1
Protein Families Druggable Genome
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Documents
Resources
"NM_173565" in other vectors (4)
SKU Description Size Price
RC220976 RSPH10B (Myc-DDK-tagged)-Human radial spoke head 10 homolog B (Chlamydomonas) (RSPH10B) 10 ug
$797.00
RC220976L3 Lenti ORF clone of Human radial spoke head 10 homolog B (Chlamydomonas) (RSPH10B), Myc-DDK-tagged 10 ug
$1,097.00
RC220976L4 Lenti ORF clone of Human radial spoke head 10 homolog B (Chlamydomonas) (RSPH10B), mGFP tagged 10 ug
$1,097.00
SC306863 RSPH10B (untagged)-Human radial spoke head 10 homolog B (Chlamydomonas) (RSPH10B) 10 ug
$877.00

Plasmid Map for RG220976

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG220976
Circular map for RG220976

file-alt Citations

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