SOX30 (NM_007017) Human Tagged ORF Clone

  • TrueORF®
SKU
RG220735
SOX30 (tGFP-tagged) - Human SRY (sex determining region Y)-box 30 (SOX30), transcript variant 2
  $665.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SOX30
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG220735 representing NM_007017
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAGAGCCAGACCCGAGCCGCCGCCTCAGCCGCGCCCGTTGCGTCCCGCTCCGCCCCCGCTGCCGG
TCGAGGGCACCTCCTTTTGGGCAGCAGCCATGGAGCCCCCTCCGTCGTCTCCCACACTGAGCGCGGCAGC
CAGTGCGACCTTGGCCTCGTCGTGCGGGGAGGCAGTGGCGTCCGGCTTACAGCCCGCGGTGCGGCGGCTG
CTGCAGGTGAAGCCAGAGCAGGTGTTGCTGCTACCACAGCCTCAGGCCCAGAACGAGGAAGCCGCTGCCT
CGTCCGCGCAGGCGCGGCTGTTGCAGTTCAGGCCCGACCTGCGGCTCCTGCAGCCGCCGACAGCGTCAGA
CGGCGCCACCTCCAGGCCCGAGTTGCACCCGGTGCAGCCCCTGGCGCTGCATGTCAAGGCCAAGAAGCAG
AAGCTGGGGCCCAGCCTGGATCAGTCAGTGGGGCCTCGAGGGGCCGTCGAAACCGGTCCTAGAGCCTCCA
GGGTGGTCAAGTTGGAAGGCCCCGGGCCGGCCCTCGGCTACTTCCGAGGGGACGAGAAGGGCAAGCTGGA
GGCGGAGGAGGTCATGAGAGACTCGATGCAAGGCGGGGCAGGCAAAAGCCCGGCAGCCATCCGAGAAGGT
GTGATCAAAACGGAGGAACCCGAGAGACTCCTCGAGGACTGCAGGCTCGGCGCGGAGCCCGCGTCCAATG
GCCTGGTTCATGGCAGCGCGGAGGTCATCTTGGCCCCAACGTCCGGTGCCTTTGGGCCGCACCAGCAAGA
CCTTAGGATCCCTTTGACGCTCCACACGGTCCCCCCTGGGGCCCGGATCCAGTTTCAGGGAGCTCCGCCT
TCAGAGCTGATAAGATTGACCAAGGTCCCCCTGACACCAGTGCCTACTAAAATGCAGTCCCTACTGGAGC
CTTCTGTAAAAATTGAAACCAAAGATGTCCCGCTCACCGTGTTGCCCTCAGATGCAGGCATACCAGATAC
TCCCTTCAGTAAGGACAGAAATGGTCATGTGAAGCGACCCATGAACGCATTTATGGTTTGGGCAAGGATC
CACCGACCAGCACTAGCCAAAGCTAACCCAGCAGCCAACAATGCAGAAATCAGTGTCCAGCTTGGGTTAG
AGTGGAACAAACTTAGTGAAGAACAAAAGAAACCCTATTACGATGAAGCACAAAAGATTAAGGAAAAGCA
CAGAGAGGAATTTCCTGGTTGGGTTTATCAGCCTCGTCCAGGGAAGCGAAAACGATTCCCTCTAAGTGTT
TCCAATGTATTTTCTGGTACCACACAGAATATCATCTCTACAAATCCTACAACAGTTTATCCTTACCGCT
CACCTACGTACTCTGTGGTAATTCCCAGCCTACAGAATCCCATCACTCATCCAGTTGTACATGCCCTTAC
AGTCGGCCTCCCTTTGGCTATGGAAATTTTCCGAGTTCAATGCCAGAATGCCTTAGTTATTATGAAGACA
GGTACCCAAAACATGAGGGTATCTTTTCAACTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG220735 representing NM_007017
Red=Cloning site Green=Tags(s)

MERARPEPPPQPRPLRPAPPPLPVEGTSFWAAAMEPPPSSPTLSAAASATLASSCGEAVASGLQPAVRRL
LQVKPEQVLLLPQPQAQNEEAAASSAQARLLQFRPDLRLLQPPTASDGATSRPELHPVQPLALHVKAKKQ
KLGPSLDQSVGPRGAVETGPRASRVVKLEGPGPALGYFRGDEKGKLEAEEVMRDSMQGGAGKSPAAIREG
VIKTEEPERLLEDCRLGAEPASNGLVHGSAEVILAPTSGAFGPHQQDLRIPLTLHTVPPGARIQFQGAPP
SELIRLTKVPLTPVPTKMQSLLEPSVKIETKDVPLTVLPSDAGIPDTPFSKDRNGHVKRPMNAFMVWARI
HRPALAKANPAANNAEISVQLGLEWNKLSEEQKKPYYDEAQKIKEKHREEFPGWVYQPRPGKRKRFPLSV
SNVFSGTTQNIISTNPTTVYPYRSPTYSVVIPSLQNPITHPVVHALTVGLPLAMEIFRVQCQNALVIMKT
GTQNMRVSFQL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007017
ORF Size 1503 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007017.2, NP_008948.1
RefSeq Size 2787 bp
RefSeq ORF 1506 bp
Locus ID 11063
UniProt ID O94993
Cytogenetics 5q33.3
Summary This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein acts as a transcriptional regulator when present in a complex with other proteins. It can activate p53 transcription to promote tumor cell apoptosis in lung cancer. The protein may be involved in the differentiation of developing male germ cells. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 5. provided by RefSeq, Apr 2015
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Documents
Resources
"NM_007017" in other vectors (4)
SKU Description Size Price
RC220735 SOX30 (Myc-DDK-tagged)-Human SRY (sex determining region Y)-box 30 (SOX30), transcript variant 2 10 ug
$466.00
RC220735L3 Lenti-ORF clone of SOX30 (Myc-DDK-tagged)-Human SRY (sex determining region Y)-box 30 (SOX30), transcript variant 2 10 ug
$766.00
RC220735L4 Lenti-ORF clone of SOX30 (mGFP-tagged)-Human SRY (sex determining region Y)-box 30 (SOX30), transcript variant 2 10 ug
$766.00
SC303866 SOX30 (untagged)-Human SRY (sex determining region Y)-box 30 (SOX30), transcript variant 2 10 ug
$514.00

Plasmid Map for RG220735

Download sequence for

Cloning scheme for RG220735

Circular map for RG220735

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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