Contactin 1 (CNTN1) (NM_175038) Human Tagged ORF Clone
$1,077.00
3 Weeks*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Contactin 1 |
| Synonyms | F3; GP135; MYPCN |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_175038 |
| ORF Size | 3021 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_175038.2, NP_778203.1 |
| RefSeq Size | 3394 bp |
| RefSeq ORF | 3024 bp |
| Locus ID | 1272 |
| UniProt ID | Q12860 |
| Cytogenetics | 12q12 |
| Protein Pathways | Cell adhesion molecules (CAMs) |
| Summary | The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Dec 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_175038" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC220493 | CNTN1 (Myc-DDK-tagged)-Human contactin 1 (CNTN1), transcript variant 2 | 10 ug |
$877.00
|
|
| RC220493L3 | Lenti ORF clone of Human contactin 1 (CNTN1), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,177.00
|
|
| RC220493L4 | Lenti ORF clone of Human contactin 1 (CNTN1), transcript variant 2, mGFP tagged | 10 ug |
$1,177.00
|
|
| SC310010 | CNTN1 (untagged)-Human contactin 1 (CNTN1), transcript variant 2 | 10 ug |
$965.00
|
|
Citations
Chronic Inflammatory Demyelinating Polyneuropathy With Concurrent Membranous Nephropathy: An Anti-paranode and Podocyte Protein Antibody Study and Literature Survey
Hashimoto, Ogata, Yamasaki et al
Front Neurol (2018) 9, 997 DOI: 10.3389/fneur.2018.00997
Hashimoto, Ogata, Yamasaki et al
Front Neurol (2018) 9, 997 DOI: 10.3389/fneur.2018.00997
AI Summary: OriGene's human CNTN1 cDNA plasmid was used to generate HEK293 cell lines expressing CNTN1-turbo GFP fusion proteins, enabling the detection of anti-CNTN1 autoantibodies in patient samples in this study.
Snippet: We generated a HEK293 cell line stably expressing human CNTN1-turbo green fluorescent protein (GFP) fusion proteins using a plasmid containing a full-length cDNA encoding human CNTN1 (OriGene Technologies Inc., Rockville, MD). The plasmids were transfected into naive HEK 293 cells using FuGENE6 (Promega Corporation, Madison, WI) according to the manufacturer's recommendations
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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