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ANKS4B (NM_145865) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG220310
ANKS4B (tGFP-tagged) - Human ankyrin repeat and sterile alpha motif domain containing 4B (ANKS4B)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ANKS4B
Synonyms HARP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG220310 representing NM_145865
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTACTCGTTACCACCAAGCTGCTAGTGATAGTTACCTGGAACTTCTAAAAGAGGCTACCAAGCGAG
ATCTAAATCTTTCGGATGAAGACGGCATGACTCCTACTCTCTTGGCAGCCTACCATGGGAACTTGGAAGC
CCTAGAGATAATCTGCAGTAGAGGAGGGGACCCTGATAGGTGTGACATCTGGGGAAACACTCCTCTACAT
TTTGCAGCCTCCAATGGCCATGCCCACTGCGTCTCATTCCTGGTCAACTTTGGTGCCAACATCTTTGCCC
TGGATAATGACTTACAGACTCCACTGGATGCTGCTGCCAGCAGGGAGCAGAATGAATGTGTTGCTCTCCT
GGACAAGGCTGCCACTGCACAGAACATCATGAACCCCAAGAAGGTCACCAGGCTGAAGGAGCAGGCTCAG
AAGAATGCCAGGAGGCAGATCAAAGAGTGTGAGAGGCTCCAGGAGAAGCACCAAAATAAGATGGCCCACA
CCTACAGCAAGGAGGAATCCGGGACTCTCTCTTCTTCCAAGGGTACCTTCTCCAGATCATCCCCTTCAAA
TGCTTCTGCTCCTGGCACATTCGGGTCACTATCTAAGGGCATTAAAGACACTTTCAAGATCAAGTTCAAG
AAGAACAAAGATACAGCAGAACAGGTGGGGAAGGAAGGCAGAAGTGGGCAGAGGAACGTGATGGAAGTGT
TCAGAGAGGAAGAGGAAGACTCGTTCTCAGGGGACTTCAAAGAGAAGCTCCAGTTGTCAGCAGAGGAGGA
CGGCAGTGTGCACCATGAATCCATTCTCAATCGTCCAGGTCTAGGAAGTATTGTTTTTAGAAGGAACAGG
ATATCGAGTCCTGAAGACATCTCAGATAGCAAGAGAGAGTTTGGTTTTAAACTGCCCAGTGAATTGCTTC
AAAGACAAGGAGCATCAGAGGCTGATGAGGGTGCAGCTGATGAAGAGGGAGAGGAAAACGGCCTCAAAGA
TGATCTGCCGTGGGATGACGATGAAGTGGAGTGGGAGGAAGATGTGGTCGATGCCACGCCCCTGGAAGTG
TTCTTGCTGTCTCAGCACCTGGAAGAATTCCTGCCTATCTTCAAGAGAGAGCAGATTGATCTAGAAGCTC
TGCTGCTCTGCTCTGATGAGGACCTTCAGAGCATACAAATGCAGCTGGGTCCCAGGAAGAAAGTTCTGAA
TGCTATCAACAGGAGGAAGCAGGTGCTTCAACAGCCTGGGCAGCTGGTCGACACCAGCCTGAGATGGAGA
GTTTTGGCCTGGAGCATTGGGGTGATGCTGTGGCCCGCTGGCAGCACTCCAGGCGGCACCCCCTCTTTAC
CCAATGCCAGACCACTGGGAATGGATTCTAGGGCATCGGAAATGCCTACC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG220310 representing NM_145865
Red=Cloning site Green=Tags(s)

MSTRYHQAASDSYLELLKEATKRDLNLSDEDGMTPTLLAAYHGNLEALEIICSRGGDPDRCDIWGNTPLH
FAASNGHAHCVSFLVNFGANIFALDNDLQTPLDAAASREQNECVALLDKAATAQNIMNPKKVTRLKEQAQ
KNARRQIKECERLQEKHQNKMAHTYSKEESGTLSSSKGTFSRSSPSNASAPGTFGSLSKGIKDTFKIKFK
KNKDTAEQVGKEGRSGQRNVMEVFREEEEDSFSGDFKEKLQLSAEEDGSVHHESILNRPGLGSIVFRRNR
ISSPEDISDSKREFGFKLPSELLQRQGASEADEGAADEEGEENGLKDDLPWDDDEVEWEEDVVDATPLEV
FLLSQHLEEFLPIFKREQIDLEALLLCSDEDLQSIQMQLGPRKKVLNAINRRKQVLQQPGQLVDTSLRWR
VLAWSIGVMLWPAGSTPGGTPSLPNARPLGMDSRASEMPT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145865
ORF Size 1380 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145865.1, NP_665872.1
RefSeq Size 2906 bp
RefSeq ORF 1254 bp
Locus ID 257629
UniProt ID Q8N8V4
Cytogenetics 16p12.2
Summary As part of the intermicrovillar adhesion complex/IMAC plays a role in epithelial brush border differentiation, controlling microvilli organization and length. Plays a role in assembly of the complex (PubMed:26812018). May play a role in cellular response to endoplasmic reticulum stress (By similarity).UniProtKB/Swiss-Prot Function
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"NM_145865" in other vectors (6)
SKU Description Size Price
RC220310 ANKS4B (Myc-DDK-tagged)-Human ankyrin repeat and sterile alpha motif domain containing 4B (ANKS4B) 10 ug
$289.00 $457.00
RC220310L1 Lenti-ORF clone of ANKS4B (Myc-DDK-tagged)-Human ankyrin repeat and sterile alpha motif domain containing 4B (ANKS4B) 10 ug
$757.00
RC220310L2 Lenti-ORF clone of ANKS4B (mGFP-tagged)-Human ankyrin repeat and sterile alpha motif domain containing 4B (ANKS4B) 10 ug
$757.00
RC220310L3 Lenti-ORF clone of ANKS4B (Myc-DDK-tagged)-Human ankyrin repeat and sterile alpha motif domain containing 4B (ANKS4B) 10 ug
$757.00
RC220310L4 Lenti-ORF clone of ANKS4B (mGFP-tagged)-Human ankyrin repeat and sterile alpha motif domain containing 4B (ANKS4B) 10 ug
$757.00
SC306281 ANKS4B (untagged)-Human ankyrin repeat and sterile alpha motif domain containing 4B (ANKS4B) 10 ug
$457.00

Plasmid Map for RG220310

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG220310
Circular map for RG220310

file-alt Citations

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