splicing factor 1 (SF1) (NM_004630) Human Tagged ORF Clone

SKU RG219995

SF1 (tGFP-tagged) - Human splicing factor 1 (SF1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: splicing factor 1
• Synonyms: BBP; D11S636; MBBP; ZCCHC25; ZFM1; ZNF162
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219995 representing NM_004630; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGACCGGAGCGAACGCCACGCCGTTGGACTTCCCAAGTAAGAAGCGGAAGAGGAGCCGCTGGAACC; AAGACACAATGGAACAGAAGACAGTGATTCCAGGAATGCCTACAGTTATTCCCCCTGGACTTACTCGAGA; ACAAGAAAGAGCTTATATAGTGCAACTGCAGATAGAAGACCTGACTCGTAAACTGCGCACAGGAGACCTG; GGCATCCCCCCTAACCCTGAGGACAGGTCCCCTTCCCCTGAGCCCATCTACAATAGCGAGGGGAAGCGGC; TTAACACCCGAGAGTTCCGCACCCGCAAAAAGCTGGAAGAGGAGCGGCACAACCTCATCACAGAGATGGT; TGCACTCAATCCGGATTTCAAGCCACCTGCAGATTACAAACCTCCAGCAACACGTGTGAGTGATAAAGTC; ATGATTCCACAAGATGAGTACCCAGAAATCAACTTTGTGGGGCTGCTCATCGGGCCCAGAGGGAACACCC; TGAAGAACATAGAGAAGGAGTGCAATGCCAAGATTATGATCCGGGGGAAAGGGTCTGTGAAAGAAGGGAA; GGTTGGGCGCAAAGATGGCCAGATGTTGCCAGGAGAAGATGAGCCACTTCATGCCCTGGTTACTGCCAAT; ACAATGGAGAACGTCAAAAAGGCAGTGGAACAGATAAGAAACATCCTGAAGCAGGGTATCGAGACTCCAG; AGGACCAGAATGATCTACGGAAGATGCAGCTTCGGGAGTTGGCTCGCTTAAATGGGACCCTTCGGGAAGA; CGATAACAGGATCTTAAGACCCTGGCAGAGCTCAGAGACCCGCAGCATTACCAACACCACAGTGTGTACC; AAGTGTGGAGGGGCTGGCCACATTGCTTCAGACTGTAAATTCCAAAGGCCTGGTGATCCTCAGTCAGCTC; AGGATAAAGCACGGATGGATAAAGAATATTTGTCCCTCATGGCTGAACTGGGTGAAGCACCTGTCCCAGC; ATCTGTGGGCTCCACCTCTGGGCCTGCCACCACACCCCTGGCCAGCGCACCTCGTCCTGCTGCTCCCGCC; AACAACCCACCTCCACCGTCTCTCATGTCTACCACCCAGAGCCGCCCACCCTGGATGAATTCTGGCCCTT; CAGAGAGTCGGCCCTACCACGGCATGCATGGAGGTGGTCCTGGTGGGCCCGGAGGTGGCCCCCACAGCTT; CCCACACCCATTACCCAGCCTGACAGGTGGGCATGGTGGACATCCCATGCAGCACAACCCCAATGGACCC; CCACCCCCTTGGATGCAGCCACCACCACCACCGATGAACCAGGGCCCCCACCCTCCTGGGCACCATGGCC; CTCCTCCAATGGATCAGTACCTGGGAAGTACGCCTGTGGGCTCTGGGGTCTATCGCCTGCATCAAGGAAA; AGGTATGATGCCGCCACCACCTATGGGCATGATGCCGCCGCCGCCGCCGCCTCCCAGTGGGCAGCCCCCA; CCCCCTCCCTCTGGTCCTCTTCCCCCATGGCAACAACAGCAGCAGCAGCCTCCGCCACCCCCTCCGCCCA; GCAGCAGTATGGCTTCCAGTACCCCCTTGCCATGGCAGCAAAATACGACGACTACCACCACGAGCGCTGG; CACAGGGTCCATCCCGCCATGGCAACAGCAGCAGGCGGCTGCCGCAGCTTCTCCAGGAGCCCCTCAGATG; CAAGGCAACCCCACTATGGTGCCCCTGCCCCCCGGGGTCCAGCCGCCTCTGCCGCCTGGGGCCCCTCCCC; CTCCGCCGCCTCCACCGCCTGGTTCCGCCGGCATGATGTATGCCCCGCCCCCTCCTCCTCCGCCTCCCAT; GGACCCTTCTAACTTTGTCACCATGATGGGCATGGGGGTGGCGGGCATGCCGCCCTTCGGGATGCCTCCA; GCTCCCCCACCGCCTCCACCACAGAAC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219995 representing NM_004630; Red=Cloning site Green=Tags(s); ; MATGANATPLDFPSKKRKRSRWNQDTMEQKTVIPGMPTVIPPGLTREQERAYIVQLQIEDLTRKLRTGDL; GIPPNPEDRSPSPEPIYNSEGKRLNTREFRTRKKLEEERHNLITEMVALNPDFKPPADYKPPATRVSDKV; MIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVKEGKVGRKDGQMLPGEDEPLHALVTAN; TMENVKKAVEQIRNILKQGIETPEDQNDLRKMQLRELARLNGTLREDDNRILRPWQSSETRSITNTTVCT; KCGGAGHIASDCKFQRPGDPQSAQDKARMDKEYLSLMAELGEAPVPASVGSTSGPATTPLASAPRPAAPA; NNPPPPSLMSTTQSRPPWMNSGPSESRPYHGMHGGGPGGPGGGPHSFPHPLPSLTGGHGGHPMQHNPNGP; PPPWMQPPPPPMNQGPHPPGHHGPPPMDQYLGSTPVGSGVYRLHQGKGMMPPPPMGMMPPPPPPPSGQPP; PPPSGPLPPWQQQQQQPPPPPPPSSSMASSTPLPWQQNTTTTTTSAGTGSIPPWQQQQAAAAASPGAPQM; QGNPTMVPLPPGVQPPLPPGAPPPPPPPPPGSAGMMYAPPPPPPPPMDPSNFVTMMGMGVAGMPPFGMPP; APPPPPPQN; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004630
• ORF Size: 1917 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004630.4
• RefSeq Size: 3527 bp
• RefSeq ORF: 1920 bp
• Locus ID: 7536
• UniProt ID: Q15637
• Cytogenetics: 11q13.1
• Domains: KH, zf-CCHC
• Protein Families: Transcription Factors
• Summary: This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]