Kv4.3 (KCND3) (NM_172198) Human Tagged ORF Clone
- TrueORF®
SKU
RG219447
KCND3 (tGFP-tagged) - Human potassium voltage-gated channel, Shal-related subfamily, member 3 (KCND3), transcript variant 2
$489.00
$792.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Kv4.3 |
| Synonyms | BRGDA9; KCND3L; KCND3S; KSHIVB; KV4.3; SCA19; SCA22 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_172198 |
| ORF Size | 1908 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_172198.1, NP_751948.1 |
| RefSeq Size | 2636 bp |
| RefSeq ORF | 1911 bp |
| Locus ID | 3752 |
| UniProt ID | Q9UK17 |
| Cytogenetics | 1p13.2 |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| Summary | Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shal-related subfamily, members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This member includes two isoforms with different sizes, which are encoded by alternatively spliced transcript variants of this gene. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_172198" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC219447 | KCND3 (Myc-DDK-tagged)-Human potassium voltage-gated channel, Shal-related subfamily, member 3 (KCND3), transcript variant 2 | 10 ug |
$289.00
$592.00
|
|
| RC219447L3 | Lenti ORF clone of Human potassium voltage-gated channel, Shal-related subfamily, member 3 (KCND3), transcript variant 2, Myc-DDK-tagged | 10 ug |
$892.00
|
|
| RC219447L4 | Lenti ORF clone of Human potassium voltage-gated channel, Shal-related subfamily, member 3 (KCND3), transcript variant 2, mGFP tagged | 10 ug |
$892.00
|
|
| SC306736 | KCND3 (untagged)-Human potassium voltage-gated channel, Shal-related subfamily, member 3 (KCND3), transcript variant 2 | 10 ug |
$593.00
|
|
Citations
Hydrogen Sulfide Targets the Cys320/Cys529 Motif in Kv4.2 to Inhibit the Ito Potassium Channels in Cardiomyocytes and Regularizes Fatal Arrhythmia in Myocardial Infarction
Ma, Luo, Ding et al
Antioxid. Redox Signal. (2015)
Ma, Luo, Ding et al
Antioxid. Redox Signal. (2015)
Cited by 26
AI Summary: OriGene products, specifically the GFP-tagged Kv4.2 (KCND2, Cat# RG215266/RC203823) and Myc-DDK-tagged KChIP2 (KCNIP2, Cat# RG219447) expression vectors, were used to express the human Ito potassium channel subunits in this study, enabling investigation of hydrogen sulfide’s regulatory effects on these channels in cardiomyocytes.
Snippet: Expression vectors The pore-forming subunit Kv4.2 (KCND2, NM-012281.2) and the auxiliary subunit KChIP2 (KCNIP2, NM-1731927) of the human Ito potassium channel were purchased from OriGene (Rockville, MD;catalogue No. RG215266/ RG219447/RC203823). Kv4.2 is a GFP-tagged ORF clone of potassium voltage-gated channel. KChIP2 is an Myc-DDKtagged ORF clone of Kv channel interacting protein 2. … We chose pIRES-hrGFP-1a as an expression vector, because this contained an IRES between the multiple cloning site and hrGFP, allowing the expression of our gene of interest, that is, KChIP2 to be monitored at the single-cell level due to expression of hrGFP on the same transcript
Showing 1-1 of 1 papers.
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