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NUSAP1 (NM_016359) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG219159
NUSAP1 (tGFP-tagged) - Human nucleolar and spindle associated protein 1 (NUSAP1), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "NUSAP1 or NM_016359"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NUSAP1
Synonyms ANKT; BM037; LNP; NUSAP; PRO0310p1; Q0310; SAPL
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG219159 representing NM_016359
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCATCCCCTCTCTAGAGGAGCTGGACTCCCTCAAGTACAGTGACCTGCAGAACTTAGCCAAGAGTC
TGGGTCTCCGGGCCAACCTGAGGGCAACCAAGTTGTTAAAAGCCTTGAAAGGCTACATTAAACATGAGGC
AAGAAAAGGAAATGAGAATCAGGATGAAAGTCAAACTTCTGCATCCTCTTGTGATGAGACTGAGATACAG
ATCAGCAACCAGGAAGAAGCTGAGAGACAGCCACTTGGCCATGTCACCAAAACAAGGAGAAGGTGCAAGA
CTGTCCGTGTGGACCCTGACTCACAGAATCATTCAGAGATAAAAATAAGTAATCCCACTGAATTCCAGAA
TCATGAAAAGCAGGAAAGCCAGGATCTCAGAGCTACTGCAAAAGTTCCTTCTCCACCAGACGAGCACCAA
GAAGCTGAGAATGCTGTTTCCTCAGGTAACAGAGATTCAAAGGTACCTTCAGAAGGAAAGAAATCTCTCT
ACACAGATGAGTCATCCAAACCTGGAAAAAATAAAAGAACTGCAATCACTACTCCAAACTTTAAGAAGCT
TCATGAAGCTCATTTTAAGGAAATGGAGTCCATTGATCAATATATTGAGAGAAAAAAGAAACATTTTGAA
GAACACAATTCCATGAATGAACTGAAGCAGCCCATCAATAAGGGAGGGGTCAGGACTCCAGTACCTCCAA
GAGGAAGACTCTCTGTGGCTTCTACTCCCATCAGCCAACGACGCTCGCAAGGCCGGTCTTGTGGCCCTGC
AAGTCAGAGTACCTTGGGTCTGAAGGGGTCACTCAAGCGCTCTGCTATCTCTGCAGCTAAAACGGGTGTC
AGGTTTTCAGCTGCTACTAAAGATAATGAGCATAAGCGTTCACTGACCAAGACTCCAGCCAGAAAGTCTG
CACATGTGACCGTGTCTGGGGGCACCCCAAAAGGCGAGGCTGTGCTTGGGACACACAAATTAAAGACCAT
CACGGGGAATTCTGCTGCTGTTATTACCCCATTCAAGTTGACAACTGAGGCAACGCAGACTCCAGTCTCC
AATAAGAAACCAGTGTTTGATCTTAAAGCAAGTTTGTCTCGTCCCCTCAACTATGAACCACACAAAGGAA
AGCTAAAACCATGGGGGCAATCTAAAGAAAATAATTATCTAAATCAACATGTCAACAGAATTAACTTCTA
CAAGAAAACTTACAAACAACCCCATCTCCAGACAAAGGAAGAGCAACGGAAGAAACGCGAGCAAGAACGA
AAGGAGAAGAAAGCAAAGGTTTTGGGAATGCGAAGGGGCCTCATTTTGGCTGAAGAT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG219159 representing NM_016359
Red=Cloning site Green=Tags(s)

MIIPSLEELDSLKYSDLQNLAKSLGLRANLRATKLLKALKGYIKHEARKGNENQDESQTSASSCDETEIQ
ISNQEEAERQPLGHVTKTRRRCKTVRVDPDSQNHSEIKISNPTEFQNHEKQESQDLRATAKVPSPPDEHQ
EAENAVSSGNRDSKVPSEGKKSLYTDESSKPGKNKRTAITTPNFKKLHEAHFKEMESIDQYIERKKKHFE
EHNSMNELKQPINKGGVRTPVPPRGRLSVASTPISQRRSQGRSCGPASQSTLGLKGSLKRSAISAAKTGV
RFSAATKDNEHKRSLTKTPARKSAHVTVSGGTPKGEAVLGTHKLKTITGNSAAVITPFKLTTEATQTPVS
NKKPVFDLKASLSRPLNYEPHKGKLKPWGQSKENNYLNQHVNRINFYKKTYKQPHLQTKEEQRKKREQER
KEKKAKVLGMRRGLILAED

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016359
ORF Size 1317 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016359.2, NP_057443.1
RefSeq Size 2288 bp
RefSeq ORF 1326 bp
Locus ID 51203
UniProt ID Q9BXS6
Cytogenetics 15q15.1
Summary NUSAP1 is a nucleolar-spindle-associated protein that plays a role in spindle microtubule organization (Raemaekers et al., 2003 PubMed 12963707).supplied by OMIM, Jun 2009
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"NM_016359" in other vectors (7)
SKU Description Size Price
RC219159 NUSAP1 (Myc-DDK-tagged)-Human nucleolar and spindle associated protein 1 (NUSAP1), transcript variant 1 10 ug
$289.00 $457.00
RC219159L1 Lenti-ORF clone of NUSAP1 (Myc-DDK-tagged)-Human nucleolar and spindle associated protein 1 (NUSAP1), transcript variant 1 10 ug
$757.00
RC219159L2 Lenti-ORF clone of NUSAP1 (mGFP-tagged)-Human nucleolar and spindle associated protein 1 (NUSAP1), transcript variant 1 10 ug
$757.00
RC219159L3 Lenti-ORF clone of NUSAP1 (Myc-DDK-tagged)-Human nucleolar and spindle associated protein 1 (NUSAP1), transcript variant 1 10 ug
$757.00
RC219159L4 Lenti-ORF clone of NUSAP1 (mGFP-tagged)-Human nucleolar and spindle associated protein 1 (NUSAP1), transcript variant 1 10 ug
$757.00
SC114338 NUSAP1 (untagged)-Human nucleolar and spindle associated protein 1 (NUSAP1), transcript variant 1 10 ug
$300.00
SC327860 NUSAP1 (untagged)-Human nucleolar and spindle associated protein 1 (NUSAP1) transcript variant 1 10 ug
$503.00

Plasmid Map for RG219159

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG219159
Circular map for RG219159

file-alt Citations

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