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MAP3K10 (NM_002446) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG218669
MAP3K10 (tGFP-tagged) - Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,510.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MAP3K10
Synonyms MEKK10; MLK2; MST
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG218669 representing NM_002446
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGGCGCGCCC

ATGGAGGAGGAGGAGGGGGCGGTGGCCAAGGAGTGGGGCACGACCCCCGCGGGGCCCGTCTGGACCGCGG
TGTTCGACTACGAGGCGGCGGGCGACGAGGAGCTGACCCTGCGGAGGGGCGATCGCGTCCAGGTGCTTTC
CCAAGACTGTGCGGTGTCCGGCGACGAGGGCTGGTGGACCGGGCAGCTCCCCAGCGGCCGCGTGGGCGTC
TTCCCCAGCAACTACGTGGCCCCCGGCGCCCCCGCTGCACCCGCGGGCCTCCAGCTGCCCCAGGAGATCC
CCTTCCACGAGCTGCAGCTAGAGGAGATCATCGGTGTGGGGGGCTTTGGCAAGGTCTATCGGGCCCTGTG
GCGTGGCGAGGAGGTGGCAGTCAAGGCCGCCCGGCTGGACCCTGAGAAGGACCCGGCAGTGACAGCGGAG
CAGGTGTGCCAGGAAGCCCGGCTCTTTGGAGCCCTGCAGCACCCCAACATAATTGCCCTTAGGGGCGCCT
GCCTCAACCCCCCACACCTCTGCCTAGTGATGGAGTATGCCCGGGGTGGTGCACTGAGCAGGGTGCTGGC
AGGTCGCCGGGTGCCACCTCACGTGCTGGTCAACTGGGCTGTGCAGGTGGCCCGGGGCATGAACTACCTA
CACAATGATGCCCCTGTGCCCATCATCCACCGGGACCTCAAGTCCATCAACATCCTGATCCTGGAGGCCA
TCGAGAACCACAACCTCGCAGACACGGTGCTCAAGATCACGGACTTCGGCCTCGCCCGCGAGTGGCACAA
GACCACCAAGATGAGCGCTGCGGGGACCTACGCCTGGATGGCGCCGGAGGTTATCCGTCTCTCCCTCTTC
TCCAAAAGCAGTGATGTCTGGAGCTTCGGGGTGCTGCTGTGGGAGCTGCTGACGGGGGAGGTCCCCTACC
GTGAGATCGACGCCTTGGCCGTGGCGTATGGCGTGGCTATGAATAAGCTGACGCTGCCCATTCCCTCCAC
GTGCCCCGAGCCCTTTGCCCGCCTCCTGGAGGAATGCTGGGACCCAGACCCCCACGGGCGGCCAGATTTC
GGTAGCATCTTGAAGCGGCTTGAAGTCATCGAACAGTCAGCCCTGTTCCAGATGCCACTGGAGTCCTTCC
ACTCGCTGCAGGAAGACTGGAAGCTGGAGATTCAGCACATGTTTGATGACCTTCGGACCAAGGAGAAGGA
GCTTCGGAGCCGTGAGGAGGAGCTGCTGCGGGCGGCACAGGAGCAGCGCTTCCAGGAGGAGCAGCTGCGG
CGGCGGGAGCAGGAGCTGGCAGAACGTGAGATGGACATCGTGGAACGGGAGCTGCACCTGCTCATGTGCC
AGCTGAGCCAGGAGAAGCCCCGGGTCCGCAAGCGCAAGGGCAACTTCAAGCGCAGCCGCCTGCTCAAGCT
GCGGGAAGGCGGCAGCCACATCAGCCTGCCCTCTGGCTTTGAGCATAAGATCACAGTCCAGGCCTCTCCA
ACTCTGGATAAGCGGAAAGGATCCGATGGGGCCAGCCCCCCTGCAAGCCCCAGCATCATCCCCCGGCTGA
GGGCCATTCGCCTGACTCCCGTGGACTGTGGTGGCAGCAGCAGTGGCAGCAGCAGTGGAGGAAGTGGGAC
ATGGAGCCGCGGTGGGCCCCCAAAGAAGGAAGAACTGGTCGGGGGCAAGAAGAAGGGACGAACGTGGGGG
CCCAGCTCCACCCTGCAGAAGGAGCGGGTGGGAGGAGAGGAGAGG

AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG218669 representing NM_002446
Red=Cloning site Green=Tags(s)

MEEEEGAVAKEWGTTPAGPVWTAVFDYEAAGDEELTLRRGDRVQVLSQDCAVSGDEGWWTGQLPSGRVGV
FPSNYVAPGAPAAPAGLQLPQEIPFHELQLEEIIGVGGFGKVYRALWRGEEVAVKAARLDPEKDPAVTAE
QVCQEARLFGALQHPNIIALRGACLNPPHLCLVMEYARGGALSRVLAGRRVPPHVLVNWAVQVARGMNYL
HNDAPVPIIHRDLKSINILILEAIENHNLADTVLKITDFGLAREWHKTTKMSAAGTYAWMAPEVIRLSLF
SKSSDVWSFGVLLWELLTGEVPYREIDALAVAYGVAMNKLTLPIPSTCPEPFARLLEECWDPDPHGRPDF
GSILKRLEVIEQSALFQMPLESFHSLQEDWKLEIQHMFDDLRTKEKELRSREEELLRAAQEQRFQEEQLR
RREQELAEREMDIVERELHLLMCQLSQEKPRVRKRKGNFKRSRLLKLREGGSHISLPSGFEHKITVQASP
TLDKRKGSDGASPPASPSIIPRLRAIRLTPVDCGGSSSGSSSGGSGTWSRGGPPKKEELVGGKKKGRTWG
PSSTLQKERVGGEER

SGPTRTRPLE - GFP Tag - V
Restriction Sites AscI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_002446
ORF Size 1725 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002446.2, NP_002437.2
RefSeq Size 3453 bp
RefSeq ORF 2865 bp
Locus ID 4294
UniProt ID Q02779
Cytogenetics 19q13.2
Protein Families Druggable Genome, Protein Kinase
Summary The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phoshorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis. provided by RefSeq, Jul 2008
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Documents
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"NM_002446" in other vectors (7)
SKU Description Size Price
RC218669 MAP3K10 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) 10 ug
$1,310.00
RC218669L1 Lenti-ORF clone of MAP3K10 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) 10 ug
$1,610.00
RC218669L2 Lenti-ORF clone of MAP3K10 (mGFP-tagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) 10 ug
$1,610.00
RC218669L3 Lenti-ORF clone of MAP3K10 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) 10 ug
$1,610.00
RC218669L4 Lenti-ORF clone of MAP3K10 (mGFP-tagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) 10 ug
$1,610.00
SC118630 MAP3K10 (untagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) 10 ug
$1,311.00
SC323447 MAP3K10 (untagged)-Kinase deficient mutant (K125M) of Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) 10 ug
$1,311.00

Plasmid Map for RG218669

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG218669
Circular map for RG218669

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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