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OSGIN1 (NM_013370) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG218475
OSGIN1 (tGFP-tagged) - Human oxidative stress induced growth inhibitor 1 (OSGIN1), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $721.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "OSGIN1 or NM_013370"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol OSGIN1
Synonyms BDGI; OKL38
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG218475 representing NM_013370
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAAGTGGAGACTGAGAGGCTGCTGCCGGGGAAATATGCAGTGCAGACAAGAGGTTCCTGCTACTC
TCACCTCATCAGAGTTGTTTTCTACCAGGACCCAGCCCCAGCCCCAGCCCCAGCCCCTGCTGGCAGATGC
TCCGGTACCCTGGGCTGTGGCTTCCAGGCTGTGCGTGACTCCAGGGCAGGGCTGTGGCCACCAGGGCCAG
GACGAGGGTCCGCTGCCAGCCCCAAGCCCCCCACCAGCCATGAGCTCCTCCAGAAAGGACCACCTCGGCG
CCAGCAGCTCAGAGCCCCTCCCGGTCATCATTGTGGGTAACGGCCCCTCTGGTATCTGCCTGTCCTACCT
GCTCTCCGGCTACACACCCTACACGAAGCCAGATGCCATCCACCCACACCCCCTGCTGCAGAGGAAGCTC
ACCGAGGCCCCGGGGGTCTCCATCCTGGACCAGGACCTGGACTACCTGTCCGAAGGCCTCGAAGGCCGAT
CCCAAAGCCCCGTGGCCCTGCTCTTTGATGCCCTTCTACGCCCAGACACAGACTTTGGGGGAAACATGAA
GTCGGTCCTCACCTGGAAGCACCGGAAGGAGCACGCCATCCCCCACGTGGTTCTGGGCCGGAACCTCCCC
GGGGGAGCCTGGCACTCCATCGAAGGCTCCATGGTGATCCTGAGCCAAGGCCAGTGGATGGGGCTCCCGG
ACCTGGAGGTCAAGGACTGGATGCAGAAGAAGCGAAGAGGTCTTCGCAACAGCCGGGCCACTGCCGGGGA
CATCGCCCACTACTACAGGGACTACGTGGTCAAGAAGGGTCTGGGGCATAACTTTGTGTCCGGTGCTGTA
GTCACAGCCGTGGAGTGGGGGACCCCCGATCCCAGCAGCTGTGGGGCCCAGGACTCCAGCCCCCTCTTCC
AGGTGAGCGGCTTCCTGACCAGGAACCAGGCCCAGCAGCCCTTCTCGCTGTGGGCCCGCAACGTGGTCCT
CGCCACAGGCACGTTCGACAGCCCGGCCCGGCTGGGCATCCCCGGGGAGGCCCTGCCCTTCATCCACCAT
GAGCTGTCTGCCCTGGAGGCCGCCACAAGGGTGGGTGCGGTGACCCCGGCCTCAGACCCTGTCCTCATCA
TTGGCGCGGGGCTGTCAGCGGCCGACGCGGTCCTCTACGCCCGCCACTACAACATCCCGGTGATCCATGC
CTTCCGCCGGGCCGTGGACGACCCTGGCCTGGTGTTCAACCAGCTGCCCAAGATGCTGTACCCCGAGTAC
CACAAGGTGCACCAGATGATGCGGGAGCAGTCCATCCTGTCGCCCAGCCCCTATGAGGGTTACCGCAGCC
TCCCCAGGCACCAGCTGCTGTGCTTCAAGGAAGACTGCCAGGCCGTGTTCCAGGACCTCGAGGGTGTCGA
GAAGGTGTTTGGGGTCTCCCTGGTGCTGGTCCTCATCGGCTCCCACCCCGACCTCTCCTTCCTGCCTGGG
GCAGGGGCTGACTTTGCAGTGGATCCTGACCAGCCGCTGAGCGCCAAGAGGAACCCCATTGACGTGGACC
CCTTCACCTACCAGAGCACCCGCCAGGAGGGCCTGTACGCCATGGGGCCGCTGGCCGGGGACAACTTCGT
GAGGTTTGTGCAGGGGGGCGCCTTGGCTGTGGCCAGCTCCCTGCTAAGGAAGGAGACCAGGAAGCCACCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG218475 representing NM_013370
Red=Cloning site Green=Tags(s)

MGKWRLRGCCRGNMQCRQEVPATLTSSELFSTRTQPQPQPQPLLADAPVPWAVASRLCVTPGQGCGHQGQ
DEGPLPAPSPPPAMSSSRKDHLGASSSEPLPVIIVGNGPSGICLSYLLSGYTPYTKPDAIHPHPLLQRKL
TEAPGVSILDQDLDYLSEGLEGRSQSPVALLFDALLRPDTDFGGNMKSVLTWKHRKEHAIPHVVLGRNLP
GGAWHSIEGSMVILSQGQWMGLPDLEVKDWMQKKRRGLRNSRATAGDIAHYYRDYVVKKGLGHNFVSGAV
VTAVEWGTPDPSSCGAQDSSPLFQVSGFLTRNQAQQPFSLWARNVVLATGTFDSPARLGIPGEALPFIHH
ELSALEAATRVGAVTPASDPVLIIGAGLSAADAVLYARHYNIPVIHAFRRAVDDPGLVFNQLPKMLYPEY
HKVHQMMREQSILSPSPYEGYRSLPRHQLLCFKEDCQAVFQDLEGVEKVFGVSLVLVLIGSHPDLSFLPG
AGADFAVDPDQPLSAKRNPIDVDPFTYQSTRQEGLYAMGPLAGDNFVRFVQGGALAVASSLLRKETRKPP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013370
ORF Size 1680 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013370.2, NP_037502.2
RefSeq Size 2400 bp
RefSeq ORF 1682 bp
Locus ID 29948
Cytogenetics 16q23.3
Summary This gene encodes an oxidative stress response protein that regulates cell death. Expression of the gene is regulated by p53 and is induced by DNA damage. The protein regulates apoptosis by inducing cytochrome c release from mitochondria. It also appears to be a key regulator of both inflammatory and anti-inflammatory molecules. The loss of this protein correlates with uncontrolled cell growth and tumor formation. Naturally occurring read-through transcription exists between this gene and the neighboring upstream malonyl-CoA decarboxylase (MLYCD) gene, but the read-through transcripts are unlikely to produce a protein product. provided by RefSeq, Aug 2011
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Documents
Resources
"NM_013370" in other vectors (4)
SKU Description Size Price
RC218475 OSGIN1 (Myc-DDK-tagged)-Human oxidative stress induced growth inhibitor 1 (OSGIN1), transcript variant 1 10 ug
$521.00
RC218475L3 Lenti ORF clone of Human oxidative stress induced growth inhibitor 1 (OSGIN1), transcript variant 1, Myc-DDK-tagged 10 ug
$821.00
RC218475L4 Lenti ORF clone of Human oxidative stress induced growth inhibitor 1 (OSGIN1), transcript variant 1, mGFP tagged 10 ug
$821.00
SC304002 OSGIN1 (untagged)-Human oxidative stress induced growth inhibitor 1 (OSGIN1), transcript variant 1 10 ug
$574.00

Plasmid Map for RG218475

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG218475
Circular map for RG218475

file-alt Citations

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