DDI2 (NM_032341) Human Tagged ORF Clone

SKU RG218468

DDI2 (tGFP-tagged) - Human DNA-damage inducible 1 homolog 2 (S. cerevisiae) (DDI2)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: DDI2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218468 representing NM_032341; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCTGCTCACCGTGTACTGTGTGCGGAGGGACCTCTCCGAGGTGACCTTTTCCCTCCAGGTCGACGCCG; ACTTCGAGCTGCACAACTTCCGCGCGCTGTGCGAGCTCGAGTCTGGCATCCCCGCAGCCGAGAGCCAGAT; CGTCTATGCGGAAAGACCTCTCACAGACAACCACAGATCATTGGCTTCTTATGGCTTGAAAGATGGGGAC; GTTGTGATTTTACGACAGAAGGAGAATGCAGACCCTCGACCTCCAGTGCAGTTCCCAAACTTACCCCGAA; TAGATTTCAGTAGTATAGCTGTGCCTGGCACATCAAGTCCCCGGCAGCGCCAGCCACCAGGAACACAGCA; GTCCCACTCATCTCCTGGAGAAATAACTTCATCTCCTCAGGGCTTGGACAATCCAGCCTTGCTCCGAGAT; ATGTTGCTGGCCAACCCGCATGAGCTGTCCTTGCTGAAGGAACGCAATCCACCCCTGGCAGAAGCTCTGC; TCAGTGGAGACCTTGAGAAATTTTCTAGAGTCCTGGTGGAGCAGCAGCAGGACCGAGCCCGGAGAGAGCA; AGAAAGGATTCGTCTGTTTTCTGCTGATCCCTTTGACCTTGAAGCTCAGGCAAAGATAGAAGAAGATATA; AGGCAACAGAACATTGAGGAAAACATGACAATAGCTATGGAAGAGGCTCCGGAAAGTTTTGGCCAAGTAG; TGATGCTTTATATTAACTGCAAAGTGAATGGACATCCTGTGAAAGCCTTTGTTGACTCAGGTGCCCAGAT; GACTATCATGAGCCAAGCTTGTGCAGAAAGGTGTAACATAATGAGACTGGTGGACCGTCGGTGGGCAGGG; ATTGCCAAAGGAGTGGGCACCCAGAAGATTATTGGAAGGGTACATCTAGCTCAGGTTCAGATTGAAGGAG; ATTTTTTGCCATGTTCCTTCTCTATACTTGAGGAACAGCCCATGGACATGCTTCTGGGACTGGACATGCT; TAAACGGCACCAGTGTTCCATCGACCTGAAGAAAAATGTACTCGTGATCGGCACCACAGGCTCCCAGACC; ACCTTTCTTCCTGAGGGAGAGCTACCAGAGTGTGCCCGGTTGGCATATGGGGCTGGAAGAGAGGATGTAC; GGCCAGAGGAGATTGCAGACCAAGAATTAGCAGAAGCCCTTCAAAAATCAGCAGAGGATGCAGAGCGTCA; GAAGCCA; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218468 representing NM_032341; Red=Cloning site Green=Tags(s); ; MLLTVYCVRRDLSEVTFSLQVDADFELHNFRALCELESGIPAAESQIVYAERPLTDNHRSLASYGLKDGD; VVILRQKENADPRPPVQFPNLPRIDFSSIAVPGTSSPRQRQPPGTQQSHSSPGEITSSPQGLDNPALLRD; MLLANPHELSLLKERNPPLAEALLSGDLEKFSRVLVEQQQDRARREQERIRLFSADPFDLEAQAKIEEDI; RQQNIEENMTIAMEEAPESFGQVVMLYINCKVNGHPVKAFVDSGAQMTIMSQACAERCNIMRLVDRRWAG; IAKGVGTQKIIGRVHLAQVQIEGDFLPCSFSILEEQPMDMLLGLDMLKRHQCSIDLKKNVLVIGTTGSQT; TFLPEGELPECARLAYGAGREDVRPEEIADQELAEALQKSAEDAERQKP; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032341
• ORF Size: 1197 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032341.5
• RefSeq Size: 1759 bp
• RefSeq ORF: 1200 bp
• Locus ID: 84301
• UniProt ID: Q5TDH0
• Cytogenetics: 1p36.21
• Protein Families: Druggable Genome
• Summary: Aspartic protease that mediates the cleavage of NFE2L1/NRF1 at 'Leu-104', thereby promoting release of NFE2L1/NRF1 from the endoplasmic reticulum membrane (PubMed:27676298, PubMed:27528193). Ubiquitination of NFE2L1/NRF1 is a prerequisite for cleavage, suggesting that DDI2 specifically recognizes and binds ubiquitinated NFE2L1/NRF1 (PubMed:27528193). Seems to act as a proteasomal shuttle which links the proteasome and replication fork proteins like RTF2 (Probable). Required, with DDI1, for cellular survival following replication stress. Together or redudantly with DDI1, removes RTF2 from stalled forks to allow cell cycle progression after replication stress and maintains genome integrity (PubMed:29290612).[UniProtKB/Swiss-Prot Function]