GATA5 (NM_080473) Human Tagged ORF Clone

  • TrueORF®
SKU
RG218399
GATA5 (tGFP-tagged) - Human GATA binding protein 5 (GATA5)
$489.00 $657.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GATA5
Synonyms bB379O24.1; CHTD5; GATAS
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG218399 representing NM_080473
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACCAGAGCCTGGCGCTGGCCGCGAGCCCCCGCCAGGCCGCCTACGCCGACTCGGGCTCCTTCCTGC
ACGCTCCGGGCGCCGGCTCTCCGATGTTTGTGCCGCCGGCGCGCGTCCCCTCGATGCTGTCCTACCTGTC
CGGGTGTGAGCCGAGCCCGCAGCCCCCCGAGCTCGCTGCGCGCCCCGGCTGGGCGCAGACAGCCACCGCG
GATTCGTCGGCCTTCGGCCCGGGCAGTCCGCACCCCCCAGCCGCGCACCCGCCCGGGGCCACCGCCTTCC
CTTTCGCGCACAGCCCCTCGGGGCCCGGCAGCGGCGGCAGCGCGGGGGGCCGAGACGGCAGTGCCTACCA
GGGCGCGCTGTTGCCTCGAGAACAGTTCGCGGCCCCGCTTGGGCGGCCGGTGGGGACCTCGTACTCCGCC
ACCTACCCGGCCTACGTGAGCCCCGACGTGGCCCAGTCCTGGACTGCCGGGCCCTTCGATGGCAGCGTCC
TGCACGGCCTCCCAGGCCGCAGGCCCACCTTCGTGTCCGACTTCTTGGAGGAGTTCCCGGGTGAGGGTCG
TGAGTGTGTCAACTGCGGGGCCCTGTCCACACCGCTGTGGCGCCGAGACGGCACCGGCCACTACCTGTGC
AATGCCTGCGGCCTCTACCACAAGATGAATGGCGTCAACCGGCCGCTCGTTCGGCCTCAGAAGCGCCTGT
CCTCGTCCCGCCGCGCCGGCCTCTGCTGCACCAACTGCCACACGACCAACACCACGCTGTGGCGGCGGAA
CTCGGAGGGGGAGCCCGTGTGCAATGCCTGCGGCCTCTACATGAAGCTGCACGGGGTGCCGCGGCCTCTG
GCTATGAAGAAAGAAAGCATCCAGACACGGAAGCGGAAGCCAAAGACCATCGCCAAGGCCAGGGGCTCCT
CAGGATCCACAAGGAATGCCTCGGCCTCCCCATCTGCTGTCGCCAGCACTGACAGCTCAGCAGCCACTTC
GAAAGCCAAGCCCAGCCTGGCGTCCCCAGTGTGCCCTGGGCCCAGCATGGCCCCCCAGGCCTCTGGCCAG
GAGGATGACTCTCTTGCCCCCGGCCACTTGGAGTTCAAGTTCGAGCCTGAGGACTTTGCCTTCCCCTCCA
CGGCCCCAAGCCCCCAGGCTGGCCTCAGGGGGGCTCTGCGCCAAGAGGCCTGGTGTGCGCTGGCCTTGGC
C


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG218399 representing NM_080473
Red=Cloning site Green=Tags(s)

MYQSLALAASPRQAAYADSGSFLHAPGAGSPMFVPPARVPSMLSYLSGCEPSPQPPELAARPGWAQTATA
DSSAFGPGSPHPPAAHPPGATAFPFAHSPSGPGSGGSAGGRDGSAYQGALLPREQFAAPLGRPVGTSYSA
TYPAYVSPDVAQSWTAGPFDGSVLHGLPGRRPTFVSDFLEEFPGEGRECVNCGALSTPLWRRDGTGHYLC
NACGLYHKMNGVNRPLVRPQKRLSSSRRAGLCCTNCHTTNTTLWRRNSEGEPVCNACGLYMKLHGVPRPL
AMKKESIQTRKRKPKTIAKARGSSGSTRNASASPSAVASTDSSAATSKAKPSLASPVCPGPSMAPQASGQ
EDDSLAPGHLEFKFEPEDFAFPSTAPSPQAGLRGALRQEAWCALALA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080473
ORF Size 1191 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080473.3, NP_536721.1
RefSeq Size 2630 bp
RefSeq ORF 1194 bp
Locus ID 140628
UniProt ID Q9BWX5
Cytogenetics 20q13.33
Protein Families Transcription Factors
Summary The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_080473" in other vectors (6)
SKU Description Size Price
RC218399 GATA5 (Myc-DDK-tagged)-Human GATA binding protein 5 (GATA5) 10 ug
$457.00
RC218399L1 Lenti ORF clone of Human GATA binding protein 5 (GATA5), Myc-DDK-tagged 10 ug
$757.00
RC218399L2 Lenti ORF clone of Human GATA binding protein 5 (GATA5), mGFP tagged 10 ug
$757.00
RC218399L3 Lenti ORF clone of Human GATA binding protein 5 (GATA5), Myc-DDK-tagged 10 ug
$757.00
RC218399L4 Lenti ORF clone of Human GATA binding protein 5 (GATA5), mGFP tagged 10 ug
$757.00
SC305786 GATA5 (untagged)-Human GATA binding protein 5 (GATA5) 10 ug
$457.00

Plasmid Map for RG218399

Download sequence for

Cloning scheme for RG218399

Circular map for RG218399

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