GREB1 (NM_148903) Human Tagged ORF Clone

  • TrueORF®
SKU
RG217865
GREB1 (tGFP-tagged) - Human growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant c
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GREB1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG217865 representing NM_148903
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAAATTCTTACGCTGGACAGCTGAAGACGACACGCTTTGAAGAGGTCTTGCACAATTCCATCGAGG
CATCCCTGCGGTCCAACAACCTGGTGCCCAGGCCCATCTTTTCCCAGCTGTACCTGGAAGCTGAGCAGCA
GCTTGCCGCTCTAGAAGGTGGTAGCCGAGTGGACAATGAGGAAGAGGAAGAAGAGGGAGAAGGAGGGCTG
GAAACAAATGGCCCCCCAACCCCTTTCCAGCTGCACCCTCTGCCTGAAGGATGCTGTACCACAGACGGGT
TTTGCCAGGCCGGGAAGGACCTGCGCCTTGTCTCCATTTCCAACGAGCCCATGGATGTCCCTGCGGGCTT
TCTCCTCGTGGGGGTCAAGTCCCCCAGCCTGCCGGACCATCTCCTGGTGTGCGCCGTTGACAAGAGGTTC
TTGCCAGATGACAATGGCCACAATGCTCTTCTTGGTTTCTCTGGGAATTGTGTTGGCTGTGGAAAGAAAG
GCTTCTGTTACTTCACGGAATTCTCCAATCATATAAATCTGAAACTGACCACTCAACCCAAGAAGCAGAA
ACACTTGAAGTATTACCTGGTCCGTAATGCACAAGGGACTCTAACCAAAGGACCTTTAATCTGTTGGAAA
GGCTCAGAGTTTAGAAGCCGGCAGATCCCCGCCAGTACTTGTTCCAGTTCCCTCTTCCCAGCCCTGGAGA
GCACGGCTGCCTTCCCCAGCGAGCCCGTTCCTGGGACGAACCCCAGCATCCTGATGGGAGCTCAGCAGGC
AGGACCAGCTTCTGATCACCCCTCACTAAACGCAGCAATGGGTCCGGCTGTTTTCAACGGCAAAGATTCC
CCGAAGTGCCAACAACTGGCAAAGAATAACCTGTTGGCCCTGCCGCGACCATCGGCTTTAGGTATCTTGT
CAAACTCCGGGCCCCCCAAAAAACGCCACAAAGGGTGGTCTCCAGAATCTCCATCAGCTCCAGATGGTGG
CTGCCCCCAAGGTGGTGGGAACAGAGCTAAGTATGAGAGCGCAGGCATGTCCTGCGTGCCGCAGGTTGGC
TTGGTGGGACCAGCTTCAGTCACCTTTCCAGTGGTGGCCTCTGGAGAACCAGTGTCTGTTCCTGACAACT
TGCTGAAAATATGCAAGGCCAAGCCAGTGATATTTAAAGTATTTGTAAATGGTGCTACCCAAATGGTAGC
CCTTGGTCCGGCAGAACCTGCATCGCCCCGGAGCTTA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG217865 representing NM_148903
Red=Cloning site Green=Tags(s)

MGNSYAGQLKTTRFEEVLHNSIEASLRSNNLVPRPIFSQLYLEAEQQLAALEGGSRVDNEEEEEEGEGGL
ETNGPPTPFQLHPLPEGCCTTDGFCQAGKDLRLVSISNEPMDVPAGFLLVGVKSPSLPDHLLVCAVDKRF
LPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGTLTKGPLICWK
GSEFRSRQIPASTCSSSLFPALESTAAFPSEPVPGTNPSILMGAQQAGPASDHPSLNAAMGPAVFNGKDS
PKCQQLAKNNLLALPRPSALGILSNSGPPKKRHKGWSPESPSAPDGGCPQGGGNRAKYESAGMSCVPQVG
LVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKVFVNGATQMVALGPAEPASPRSL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_148903
ORF Size 1227 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_148903.1, NP_683701.1
RefSeq Size 2341 bp
RefSeq ORF 1230 bp
Locus ID 9687
UniProt ID Q4ZG55
Cytogenetics 2p25.1
Summary This gene is an estrogen-responsive gene that is an early response gene in the estrogen receptor-regulated pathway. It is thought to play an important role in hormone-responsive tissues and cancer. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_148903" in other vectors (4)
SKU Description Size Price
RC217865 GREB1 (Myc-DDK-tagged)-Human growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant c 10 ug
$457.00
RC217865L3 Lenti ORF clone of Human growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant c, Myc-DDK-tagged 10 ug
$757.00
RC217865L4 Lenti ORF clone of Human growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant c, mGFP tagged 10 ug
$757.00
SC306330 GREB1 (untagged)-Human growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant c 10 ug
$503.00

Plasmid Map for RG217865

Download sequence for

Cloning scheme for RG217865

Circular map for RG217865

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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