H2BC4 (NM_003526) Human Tagged ORF Clone

SKU RG217796

HIST1H2BC (tGFP-tagged) - Human histone cluster 1, H2bc (HIST1H2BC)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: H2BC4
• Synonyms: dJ221C16.3; H2B.1; H2B/l; H2BC6; H2BC7; H2BC8; H2BC10; H2BFL; HIST1H2BC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217796 representing NM_003526; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCCTGAGCCAGCCAAGTCTGCTCCCGCCCCGAAGAAGGGCTCCAAGAAGGCAGTGACCAAAGCGCAGA; AGAAAGATGGCAAGAAGCGCAAGCGCAGCCGCAAGGAGAGTTACTCTGTGTACGTGTACAAGGTGCTGAA; ACAGGTCCATCCCGACACTGGCATCTCTTCCAAGGCCATGGGCATCATGAATTCTTTCGTTAACGACATA; TTTGAGCGCATCGCGGGCGAGGCTTCCCGCCTGGCGCATTACAACAAGCGCTCGACCATCACCTCCAGGG; AGATCCAGACGGCCGTGCGCCTGCTGCTTCCCGGAGAGCTGGCCAAGCACGCCGTGTCGGAGGGCACCAA; GGCCGTCACCAAGTACACCAGCTCCAAG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217796 representing NM_003526; Red=Cloning site Green=Tags(s); ; MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDI; FERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003526
• ORF Size: 378 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003526.3
• RefSeq Size: 438 bp
• RefSeq ORF: 381 bp
• Locus ID: 8347
• UniProt ID: P62807
• Cytogenetics: 6p22.2
• Domains: H2B, histone
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Systemic lupus erythematosus
• Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. The protein has antibacterial and antifungal antimicrobial activity. The main transcript variant of this gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. This transcript variant lacks a polyA tail but instead contains a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Apr 2020]