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SDCCAG8 (NM_006642) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG217089
SDCCAG8 (tGFP-tagged) - Human serologically defined colon cancer antigen 8 (SDCCAG8)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $853.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SDCCAG8
Synonyms BBS16; CCCAP; CCCAP SLSN7; hCCCAP; HSPC085; NPHP10; NY-CO-8; SLSN7
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG217089 representing NM_006642
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAAGTCCCCGGAGAACTCTACCCTGGAGGAGATTCTGGGGCAGTATCAACGGAGTCTCCGGGAAC
ATGCCAGCAGAAGCATTCACCAACTGACATGTGCCCTGAAAGAAGGCGATGTCACTATTGGAGAAGATGC
ACCAAATCTTTCTTTTAGCACCAGTGTGGGAAATGAGGACGCCAGGACAGCCTGGCCCGAATTACAACAG
AGCCATGCTGTTAATCAGCTCAAAGATTTGTTGCGCCAACAAGCAGATAAGGAAAGTGAAGTATCTCCGT
CAAGAAGAAGAAAAATGTCCCCCTTGAGGTCATTAGAACATGAGGAAACCAATATGCCTACTATGCACGA
CCTTGTTCATACTATTAATGACCAGTCTCAATATATTCATCATTTAGAGGCAGAAGTTAAGTTCTGCAAG
GAGGAACTCTCTGGAATGAAAAATAAAATACAAGTAGTTGTGCTTGAAAACGAAGGGCTCCAGCAACAGC
TAAAATCTCAAAGACAAGAGGAGACACTGAGGGAACAAACACTTCTGGATGCATCCGGAAACATGCACAA
TTCTTGGATTACAACAGGTGAAGATTCTGGGGTGGGCGAAACCTCCAAAAGACCATTTTCCCATGACAAT
GCAGATTTTGGCAAAGCTGCATCTGCTGGTGAGCAGCTAGAACTGGAGAAGCTAAAACTTACTTATGAGG
AAAAGTGTGAAATTGAGGAATCCCAATTGAAGTTTTTGAGGAACGACTTAGCTGAATATCAGAGAACTTG
TGAAGATCTTAAAGAGCAACTAAAGCATAAAGAATTTCTTCTGGCTGCTAATACTTGTAACCGTGTTGGT
GGTCTTTGTTTGAAATGTGCTCAGCATGAAGCTGTTCTTTCCCAAACCCATACTAATGTTCATATGCAGA
CCATCGAAAGACTGGTTAAAGAAAGAGATGACTTGATGTCTGCACTAGTTTCCGTAAGGAGCAGCTTGGC
AGATACGCAGCAAAGAGAAGCAAGTGCTTATGAACAGGTGAAACAAGTTTTGCAAATATCTGAGGAAGCC
AATTTTGAAAAAACCAAGGCTTTAATCCAGTGTGACCAGTTGAGGAAGGAGCTGGAGAGGCAGGCGGAGC
GACTTGAAAAAGAACTTGCATCTCAGCAAGAGAAAAGGGCCATTGAGAAAGACATGATGAAAAAGGAAAT
AACGAAAGAAAGGGAGTACATGGGATCAAAGATGTTGATCTTGTCTCAGAATATTGCCCAACTGGAGGCC
CAGGTGGAAAAGGTTACAAAGGAAAAGATTTCAGCTATTAATCAACTGGAGGAAATTCAAAGCCAGCTGG
CTTCTCGGGAAATGGATGTCACAAAGGTGTGTGGAGAAATGCGCTATCAGCTGAATAAAACCAACATGGA
GAAGGATGAGGCAGAAAAGGAGCACAGAGAGTTCAGAGCAAAAACTAACAGGGATCTTGAAATTAAAGAT
CAGGAAATAGAGAAATTGAGAATAGAACTGGATGAAAGCAAACAACACTTGGAACAGGAGCAGCAGAAGG
CAGCCCTGGCCAGAGAGGAGTGCCTGAGACTAACAGAACTGCTGGGCGAATCTGAGCACCAACTGCACCT
CACCAGACAGGAAAAAGATAGCATTCAGCAGAGCTTTAGCAAGGAAGCAAAGGCCCAAGCCCTTCAGGCC
CAGCAAAGAGAGCAGGAGCTGACACAGAAGATACAGCAAATGGAAGCCCAGCATGACAAAACTGAAAATG
AACAGTATTTGTTGCTGACCTCCCAGAATACATTTTTGACAAAGTTAAAGGAAGAATGCTGTACATTAGC
CAAGAAACTGGAACAAATCTCTCAAAAAACCAGATCTGAAATAGCTCAACTCAGTCAAGAAAAAAGGTAT
ACATATGATAAATTGGGAAAGTTACAGAGAAGAAATGAAGAATTGGAGGAACAGTGTGTCCAGCATGGGA
GAGTACATGAGACGATGAAGCAAAGGCTAAGGCAGCTGGATAAGCACAGCCAGGCCACAGCCCAGCAGCT
GGTGCAGCTCCTCAGCAAGCAGAACCAGCTTCTCCTGGAGAGGCAGAGCCTGTCGGAAGAGGTGGACCGG
CTGCGGACCCAGTTACCCAGCATGCCACAATCTGATTGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG217089 representing NM_006642
Red=Cloning site Green=Tags(s)

MAKSPENSTLEEILGQYQRSLREHASRSIHQLTCALKEGDVTIGEDAPNLSFSTSVGNEDARTAWPELQQ
SHAVNQLKDLLRQQADKESEVSPSRRRKMSPLRSLEHEETNMPTMHDLVHTINDQSQYIHHLEAEVKFCK
EELSGMKNKIQVVVLENEGLQQQLKSQRQEETLREQTLLDASGNMHNSWITTGEDSGVGETSKRPFSHDN
ADFGKAASAGEQLELEKLKLTYEEKCEIEESQLKFLRNDLAEYQRTCEDLKEQLKHKEFLLAANTCNRVG
GLCLKCAQHEAVLSQTHTNVHMQTIERLVKERDDLMSALVSVRSSLADTQQREASAYEQVKQVLQISEEA
NFEKTKALIQCDQLRKELERQAERLEKELASQQEKRAIEKDMMKKEITKEREYMGSKMLILSQNIAQLEA
QVEKVTKEKISAINQLEEIQSQLASREMDVTKVCGEMRYQLNKTNMEKDEAEKEHREFRAKTNRDLEIKD
QEIEKLRIELDESKQHLEQEQQKAALAREECLRLTELLGESEHQLHLTRQEKDSIQQSFSKEAKAQALQA
QQREQELTQKIQQMEAQHDKTENEQYLLLTSQNTFLTKLKEECCTLAKKLEQISQKTRSEIAQLSQEKRY
TYDKLGKLQRRNEELEEQCVQHGRVHETMKQRLRQLDKHSQATAQQLVQLLSKQNQLLLERQSLSEEVDR
LRTQLPSMPQSDC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006642
ORF Size 2139 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006642.1, NP_006633.1
RefSeq Size 2142 bp
RefSeq ORF 2142 bp
Locus ID 10806
UniProt ID Q86SQ7
Cytogenetics 1q43-q44
Summary This gene encodes a centrosome associated protein. This protein may be involved in organizing the centrosome during interphase and mitosis. Mutations in this gene are associated with retinal-renal ciliopathy. provided by RefSeq, Oct 2010
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"NM_006642" in other vectors (4)
SKU Description Size Price
RC217089 SDCCAG8 (Myc-DDK-tagged)-Human serologically defined colon cancer antigen 8 (SDCCAG8) 10 ug
$653.00
RC217089L3 Lenti ORF clone of Human serologically defined colon cancer antigen 8 (SDCCAG8), Myc-DDK-tagged 10 ug
$953.00
RC217089L4 Lenti ORF clone of Human serologically defined colon cancer antigen 8 (SDCCAG8), mGFP tagged 10 ug
$953.00
SC314504 SDCCAG8 (untagged)-Human serologically defined colon cancer antigen 8 (SDCCAG8) 10 ug
$719.00

Plasmid Map for RG217089

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG217089
Circular map for RG217089

file-alt Citations

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