FMN1 (NM_001103184) Human Tagged ORF Clone

  • TrueORF®
SKU
RG216074
FMN1 (tGFP-tagged) - Human formin 1 (FMN1)
  $1,761.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FMN1
Synonyms FMN; LD
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001103184
ORF Size 3588 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001103184.4
RefSeq Size 3757 bp
RefSeq ORF 3591 bp
Locus ID 342184
UniProt ID Q68DA7
Cytogenetics 15q13.3
Summary This gene belongs to the formin homology family and encodes a protein that has a role in the formation of adherens junction and the polymerization of linear actin cables. The homologous gene in mouse is associated with limb deformity. Alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Sep 2015
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Resources
"NM_001103184" in other vectors (4)
SKU Description Size Price
RC216074 FMN1 (Myc-DDK-tagged)-Human formin 1 (FMN1) 10 ug
$1,561.00
RC216074L3 Lenti ORF clone of Human formin 1 (FMN1), Myc-DDK-tagged 10 ug
$1,861.00
RC216074L4 Lenti ORF clone of Human formin 1 (FMN1), mGFP tagged 10 ug
$1,861.00
SC316979 FMN1 (untagged)-Human formin 1 (FMN1) 10 ug
$1,667.00

Plasmid Map for RG216074

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Cloning scheme for RG216074

Circular map for RG216074

file-alt Citations

DKK1 is a potential novel mediator of cisplatin-refractoriness in non-small cell lung cancer cell lines
Salim, Zong, Hååg et al
BMC Cancer (2015) 15 (1), 628 DOI: 10.1186/s12885-015-1635-9
Cited by 37
AI Summary: OriGene’s pCMV6-AC-GFP-FMN1 plasmid was used to overexpress FMN1 in U-1810 cells, enabling the study of FMN1’s role in cisplatin-refractoriness in non-small cell lung cancer.
Snippet: FMN1 was overexpressed in U-1810 cells by transfecting cells with the FMN1 open reading frame cDNA integrated in the pCMV6-AC-GFP plasmid (OriGene, Rockville, Maryland, USA), using Lipofectamine LTX reagent (Invitrogen, Germany). ... Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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