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KLHL30 (NM_198582) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG215869
KLHL30 (tGFP-tagged) - Human kelch-like 30 (Drosophila) (KLHL30)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $738.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KLHL30
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG215869 representing NM_198582
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGATGGCCTGCAGCGCCTGCGCTCTCAGCCCAAGCTGGCCGACGTCACACTGCTGGTGGGCGGCC
GGGAGCTGCCATGCCACCGCGGCCTCCTGGCGCTCAGCAGCCCCTACTTCCATGCCATGTTTGCGGGTGA
CTTCGCCGAGAGCTTCTCTGCGCGCGTGGAGCTGCGGGACGTGGAGCCCGCCGTGGTGGGACAACTGGTG
GACTTCGTGTACACAGGCCGGCTGACCATCACGCAGGGCAACGTGGAGGCGCTGACACGCACGGCTGCGC
GCCTGCACTTCCCCTCGGTGCAGAAGGTCTGCGGCCGCTACCTGCAGCAGCAACTGGATGCCGCCAACTG
CCTGGGCATCTGTGAGTTCGGGGAGCAGCAAGGGCTGCTGGGCGTGGCTGCCAAGGCCTGGGCCTTCCTG
CGAGAGAACTTTGAGGCTGTGGCACGTGAGGACGAGTTCCTGCAGCTTCCCCGAGAGCGGCTGGTCACTT
GTCTGGCCGGCGACCTGCTGCAGGTACAGCCGGAGCAAAGCCGACTCGAGGCCCTGATGCGCTGGGTGCG
CCATGACCCGCAGGCCCGGGCCGCCCACCTGCCCGAGCTGCTCAGCCTAGTGCACCTGGACGCCGTGCCC
AGGCCCTGCGTGCAGCAACTGCTGGCCTCAGAGCCCCTGATCCAGGAGTCAGAGGCATGCCGGGCAGCCC
TGTCCCAGGGCCATGATGGGGCACCACTCGCCCTCCAGCAGAAGCTGGAGGAGGTCCTGGTGGTGGTGGG
CGGGCAGGCGCTGGAGGAGGAGGAGGCAGGTGAGGAGCCCACCCCCGGCCTTGGGAACTTTGCCTTCTAC
AACAGCAAGGCCAAGAGGTGGATGGCACTTCCAGACTTCCCCGACTATCACAAGTGGGGTTTCTCCCTGG
CGGCCCTGAACAACAACATCTATGTCACAGGTGGCTCTCGGGGCACAAAGACAGACACCTGGTCAACCAC
CCAGGCCTGGTGCTTCCCCCTGAAGGAGGCCTCCTGGAAGCCCGTGGCGCCCATGCTGAAGCCCCGCACC
AACCACGCCAGCGCGGCCCTCAATGGGGAGATCTACGTTATCGGCGGCACCACCCTGGACGTGGTGGAGG
TGGAGAGCTATGACCCCTACACGGACAGCTGGACGCCCGTCAGCCCGGCCCTCAAATACGTCAGCAACTT
CTCGGCTGCCGGCTGCCGGGGCCGGCTCTACCTGGTGGGCTCCAGCGCCTGCAAGTACAACGCCCTGGCC
CTGCAGTGCTACAACCCTGTCACAGATGCGTGGAGTGTGATCGCCTCGCCCTTCCTGCCCAAGTACCTGT
CCTCGCCTCGCTGTGCTGCACTGCACGGGGAGCTCTACCTCATTGGGGACAACACCAAGAAGGTCTACGT
GTACGACCCCGGGGCCAACCTGTGGCAGAAGGTGCAGTCACAGCACAGCCTGCATGAGAATGGCGCGCTG
GTGCCACTGGGTGATGCGCTGTACGTGACGGGCGGCCGCTGGCAGGGCATGGAAGGTGACTACCACGTGG
AGATGGAGGCCTACGACACGGTTCGGGACACCTGGACCCGCCACGGCGCCCTGCCCCGGCTCTGGCTCTA
CCACGGGGCCTCCACCGTCTTCCTGGATGTCTCCAAGTGGACCCAGCCCTCCGGCCCCACCCAGGAGCAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG215869 representing NM_198582
Red=Cloning site Green=Tags(s)

MLDGLQRLRSQPKLADVTLLVGGRELPCHRGLLALSSPYFHAMFAGDFAESFSARVELRDVEPAVVGQLV
DFVYTGRLTITQGNVEALTRTAARLHFPSVQKVCGRYLQQQLDAANCLGICEFGEQQGLLGVAAKAWAFL
RENFEAVAREDEFLQLPRERLVTCLAGDLLQVQPEQSRLEALMRWVRHDPQARAAHLPELLSLVHLDAVP
RPCVQQLLASEPLIQESEACRAALSQGHDGAPLALQQKLEEVLVVVGGQALEEEEAGEEPTPGLGNFAFY
NSKAKRWMALPDFPDYHKWGFSLAALNNNIYVTGGSRGTKTDTWSTTQAWCFPLKEASWKPVAPMLKPRT
NHASAALNGEIYVIGGTTLDVVEVESYDPYTDSWTPVSPALKYVSNFSAAGCRGRLYLVGSSACKYNALA
LQCYNPVTDAWSVIASPFLPKYLSSPRCAALHGELYLIGDNTKKVYVYDPGANLWQKVQSQHSLHENGAL
VPLGDALYVTGGRWQGMEGDYHVEMEAYDTVRDTWTRHGALPRLWLYHGASTVFLDVSKWTQPSGPTQEH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198582
ORF Size 1680 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198582.2, NP_940984.2
RefSeq Size 3557 bp
RefSeq ORF 1737 bp
Locus ID 377007
UniProt ID Q0D2K2
Cytogenetics 2q37.3
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Documents
Resources
"NM_198582" in other vectors (4)
SKU Description Size Price
RC215869 KLHL30 (Myc-DDK-tagged)-Human kelch-like 30 (Drosophila) (KLHL30) 10 ug
$538.00
RC215869L3 Lenti ORF clone of Human kelch-like 30 (Drosophila) (KLHL30), Myc-DDK-tagged 10 ug
$838.00
RC215869L4 Lenti ORF clone of Human kelch-like 30 (Drosophila) (KLHL30), mGFP tagged 10 ug
$838.00
SC317962 KLHL30 (untagged)-Human kelch-like 30 (Drosophila) (KLHL30) 10 ug
$593.00

Plasmid Map for RG215869

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG215869
Circular map for RG215869

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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