OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

GPR35 (NM_005301) Human Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
RG215174
GPR35 (tGFP-tagged) - Human G protein-coupled receptor 35 (GPR35), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $500.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA313953 Rabbit polyclonal anti-GPR35 antibody
100 ul
$380.00
TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8 100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "GPR35 or NM_005301"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GPR35
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG215174 representing NM_005301
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGGCACCTACAACACCTGTGGCTCCAGCGACCTCACCTGGCCCCCAGCGATCAAGCTGGGCTTCT
ACGCCTACTTGGGCGTCCTGCTGGTGCTAGGCCTGCTGCTCAACAGCCTGGCGCTCTGGGTGTTCTGCTG
CCGCATGCAGCAGTGGACGGAGACCCGCATCTACATGACCAACCTGGCGGTGGCCGACCTCTGCCTGCTG
TGCACCTTGCCCTTCGTGCTGCACTCCCTGCGAGACACCTCAGACACGCCGCTGTGCCAGCTCTCCCAGG
GCATCTACCTGACCAACAGGTACATGAGCATCAGCCTGGTCACGGCCATCGCCGTGGACCGCTATGTGGC
CGTGCGGCACCCGCTGCGTGCCCGCGGGCTGCGGTCCCCCAGGCAGGCTGCGGCCGTGTGCGCGGTCCTC
TGGGTGCTGGTCATCGGCTCCCTGGTGGCTCGCTGGCTCCTGGGGATTCAGGAGGGCGGCTTCTGCTTCA
GGAGCACCCGGCACAATTTCAACTCCATGGCGTTCCCGCTGCTGGGATTCTACCTGCCCCTGGCCGTGGT
GGTCTTCTGCTCCCTGAAGGTGGTGACTGCCCTGGCCCAGAGGCCACCCACCGACGTGGGGCAGGCAGAG
GCCACCCGCAAGGCTGCCCGCATGGTCTGGGCCAACCTCCTGGTGTTCGTGGTCTGCTTCCTGCCCCTGC
ACGTGGGGCTGACAGTGCGCCTCGCAGTGGGCTGGAACGCCTGTGCCCTCCTGGAGACGATCCGTCGCGC
CCTGTACATAACCAGCAAGCTCTCAGATGCCAACTGCTGCCTGGACGCCATCTGCTACTACTACATGGCC
AAGGAGTTCCAGGAGGCGTCTGCACTGGCCGTGGCTCCCAGTGCTAAGGCCCACAAAAGCCAGGACTCTC
TGTGCGTGACCCTCGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG215174 representing NM_005301
Red=Cloning site Green=Tags(s)

MNGTYNTCGSSDLTWPPAIKLGFYAYLGVLLVLGLLLNSLALWVFCCRMQQWTETRIYMTNLAVADLCLL
CTLPFVLHSLRDTSDTPLCQLSQGIYLTNRYMSISLVTAIAVDRYVAVRHPLRARGLRSPRQAAAVCAVL
WVLVIGSLVARWLLGIQEGGFCFRSTRHNFNSMAFPLLGFYLPLAVVVFCSLKVVTALAQRPPTDVGQAE
ATRKAARMVWANLLVFVVCFLPLHVGLTVRLAVGWNACALLETIRRALYITSKLSDANCCLDAICYYYMA
KEFQEASALAVAPSAKAHKSQDSLCVTLA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005301
ORF Size 927 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005301.3
RefSeq Size 1875 bp
RefSeq ORF 930 bp
Locus ID 2859
UniProt ID Q9HC97
Cytogenetics 2q37.3
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
Summary Acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of this receptor is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_005301" in other vectors (6)
SKU Description Size Price
RC215174 GPR35 (Myc-DDK-tagged)-Human G protein-coupled receptor 35 (GPR35), transcript variant 1 10 ug
$289.00 $300.00
RC215174L1 Lenti ORF clone of Human G protein-coupled receptor 35 (GPR35), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC215174L2 Lenti ORF clone of Human G protein-coupled receptor 35 (GPR35), transcript variant 1, mGFP tagged 10 ug
$600.00
RC215174L3 Lenti ORF clone of Human G protein-coupled receptor 35 (GPR35), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC215174L4 Lenti ORF clone of Human G protein-coupled receptor 35 (GPR35), transcript variant 1, mGFP tagged 10 ug
$600.00
SC116808 GPR35 (untagged)-Human G protein-coupled receptor 35 (GPR35), transcript variant 1 10 ug
$300.00

Plasmid Map for RG215174

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG215174
Circular map for RG215174

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products