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GPR35 (NM_005301) Human Untagged Clone

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SKU
SC116808
GPR35 (untagged)-Human G protein-coupled receptor 35 (GPR35), transcript variant 1
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol GPR35
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>SC116808 representing NM_005301.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCGGCCGCGAATTCGGCACGAGCTTTGGCAGCGGGGGTCACACACTCCACCCGGGAGGCCAAAGCTGCC
TGCAGGACCATGAATGGCACCTACAACACCTGTGGCTCCAGCGACCTCACCTGGCCCCCAGCGATCAAG
CTGGGCTTCTACGCCTACTTGGGCGTCCTGCTGGTGCTAGGCCTGCTGCTCAACAGCCTGGCGCTCTGG
GTGTTCTGCTGCCGCATGCAGCAGTGGACGGAGACCCGCATCTACATGACCAACCTGGCGGTGGCCGAC
CTCTGCCTGCTGTGCACCTTGCCCTTCGTGCTGCACTCCCTGCGAGACACCTCAGACACGCCGCTGTGC
CAGCTCTCCCAGGGCATCTACCTGACCAACAGGTACATGAGCATCAGCCTGGTCACGGCCATCGCCGTG
GACCGCTATGTGGCCGTGCGGCACCCGCTGCGTGCCCGCGGGCTGCGGTCCCCCAGGCAGGCTGCGGCC
GTGTGCGCGGTCCTCTGGGTGCTGGTCATCGGCTCCCTGGTGGCTCGCTGGCTCCTGGGGATTCAGGAG
GGCGGCTTCTGCTTCAGGAGCACCCGGCACAATTTCAACTCCATGGCGTTCCCGCTGCTGGGATTCTAC
CTGCCCCTGGCCGTGGTGGTCTTCTGCTCCCTGAAGGTGGTGACTGCCCTGGCCCAGAGGCCACCCACC
GACGTGGGGCAGGCAGAGGCCACCCGCAAGGCTGCCCGCATGGTCTGGGCCAACCTCCTGGTGTTCGTG
GTCTGCTTCCTGCCCCTGCACGTGGGGCTGACAGTGCGCCTCGCAGTGGGCTGGAACGCCTGTGCCCTC
CTGGAGACGATCCGTCGCGCCCTGTACATAACCAGCAAGCTCTCAGATGCCAACTGCTGCCTGGACGCC
ATCTGCTACTACTACATGGCCAAGGAGTTCCAGGAGGCGTCTGCACTGGCCGTGGCTCCCAGTGCTAAG
GCCCACAAAAGCCAGGACTCTCTGTGCGTGACCCTCGCCTAAGAGGCGTGCTGTGGGCGCTGTGGGCCA
GGTCTCGGGGGCTCCGGGAGGTGCTGCCTGCCAGGGGAAGCTGGAACCAGTAGCAAGGAGCCCGAGATC
AGCCCTGAACTCACTGTGTATTCTCTTGGAGCCTTGGGTGGGCAGGGACGGCCCAGGTACCTGCTCTCT
TGGGAAGAGAGAGGGACAGGGACAAGGGCAAGAGGACTGAGGCCAGAGCAAGGCCAATGTCAGAGACCC
CCGGGATGGGGCCTCACACTTGCCACCCCCAGAACCAGCTCACCTGGCCAGAGTGGGTTCCTGCTGGCC
AGGGTGCAGCCTTGATGACACCTGCCGCTGCCCCTCGGGGCTGGAATAAAACTCCCCACCCAGAGTCAA
AAAAAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_005301 unedited
NATTTTGTNATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCTTTGGCAGCGG
GGGTCACACACTCCACCCGGGAGGCCAAAGCTGCCTGCAGGACCATGAATGGCACCTACA
ACACCTGTGGCTCCAGCGACCTCACCTGGCCCCCAGCGATCAAGCTGGGCTTCTACGCCT
ACTTGGGCGTCCTGCTGGTGCTAGGCCTGCTGCTCAACAGCCTGGCGCTCTGGGTGTTCT
GCTGCCGCATGCAGCAGTGGACGGAGACCCGCATCTACATGACCAACCTGGCGGTGGCCG
ACCTCTGCCTGCTGTGCACCTTGCCCTTCGTGCTGCACTCCCTGCGAGACACCTCAGACA
CGCCGCTGTGCCAGCTCTCCCAGGGCATCTACCTGACCAACAGGTACATGAGCATCAGCC
TGGTCACGGCCATCGCCGTGGACCGCTATGTGGCCGTGCGGCACCCGCTGCGTGCCCGCG
GGCTGCGGTCCCCCAGGCAGGCTGCGGCCGTGTGCGCGGTCCTCTGGGTGCTGGTCATCG
GCTCCCTGGTGGCTCGCTGGCTCCTGGGGATTCAGGAGGGCGGCTTCTGCTTCAGGAGCA
CCCGGCACAATTTCAACTCCATGGCGTTCCCGCTGCTGGGATTCTACCTGCCCCTGGCCG
TGGTGGTCTTCTGCTCCCTGAAGGTGGTGACTGCCCTGGCCCAGAGGCCACCCACCGACG
TGGGGCAGGCAGAGGCCACCCGCAAGGCTGCCCGCATGGTCTGGGCCAACCTCCTGGNTG
TTCGTGGTCTGCTTCCTGCCCCTGCACGTGGNGCTGACAGTGCGCCTCGCAGTGGGCTGG
AACGCCCTGTGCCTCCTGGAGACGATCCGTCGCGCCTGTACATACCAGCAGCTCTCAGAT
GCACTGCTGCTGNACGCATCTGCTCTACTACTGGNNCAGAGTNCAGNNAGCGCTGACTGG
CCGTGCTCCAGTGCTAAGCCACAN
3' Read Nucleotide Sequence
>OriGene 3' genomic read for NM_005301 unedited
NTTCGTGGGNCCGCGGCCGTTTTCTATTATCGAGTTTTTTTTTTTTTTTTTTTTTTTTCT
TTGACTCTGGGTGGGGAGACCCAATCCAGCCCCGAGGGGCAGCGGCACCNCCNCCNCCGG
CATTTTTTTTTGTTTNNTGAANTACTCTGGCCAGGCGAACTGGTTCCGGGGGTGCCTCTT
GTGCCGTCCCCTCCCCCCGGCCCTTCAATTGGCCTTTTTCNTCCCCTCCCCCTTCCTCCC
TGCTCCTGCCCCTCCTCTCTTCCCAAGAGAGCAGGTACCTGGGCCGTCCCTGCCCACCCA
AGGGTCCAAGAGAATACACAGCGAGTTCAGGGCTGATCTCGGGCTTCTTGCTACTGGTTC
CAGCTTCCCCTGGCAGGCAGGACCTCCCGGAGCCCCTGAGACCTGGCCCACAGCGCCCAC
AGTACGCCTCTTATGCGAGGGGCACGCACAGAGAGTCCTGGCTTTTGTGGGCCTTATCAC
TGGGAGCCACGGGCAGTGGAGAAGCCTTCTGGAACTCCTTGGCCATGTTCTTATACCAGA
TGGCGGCCAGGCACCATTCGGCCTCCGAGAAGCTGCCGGTTATGTTCAGGGCGCGACTGA
CTGTCTCCACGACGGCACAGGTGGTTCAGCCCATTGGCAGGGGTACTGTCCGCCACACGT
GCATGGGCAGTGAGAAATCCCGACATCAAGAGGGGTGGCCAACACAAGCGGGCAGTCTTG
CGGGGGTCTCTGTCCGCCCCAGTGTGGGGTGGGCTTTGGGCAAGGAGTCCCCACCTTTAG
GGACAACAGCCACCCCGGCAGGGCAGGTAAAACTCACCGGGGAACGCCTGGAATGTAATT
GGCCGGGTGCTTCTGAGCAAAAACGCCTTTCTGTATCCCAGAACCACGAAGCCCCCAGGA
ACCGTGACCATCACCTACGGG
Restriction Sites NotI-NotI |
ACCN NM_005301
Insert Size 1427 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005301.2
RefSeq Size 1875 bp
RefSeq ORF 1427 bp
Locus ID 2859
UniProt ID Q9HC97
Cytogenetics 2q37.3
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 51.4 kDa
Summary Acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of this receptor is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the shortest transcript and encodes the shorter isoform (a, also known as GPR35a).
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Documents
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"NM_005301" in other vectors (6)
SKU Description Size Price
RC215174 GPR35 (Myc-DDK-tagged)-Human G protein-coupled receptor 35 (GPR35), transcript variant 1 10 ug
$289.00 $300.00
RC215174L1 Lenti ORF clone of Human G protein-coupled receptor 35 (GPR35), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC215174L2 Lenti ORF clone of Human G protein-coupled receptor 35 (GPR35), transcript variant 1, mGFP tagged 10 ug
$600.00
RC215174L3 Lenti ORF clone of Human G protein-coupled receptor 35 (GPR35), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC215174L4 Lenti ORF clone of Human G protein-coupled receptor 35 (GPR35), transcript variant 1, mGFP tagged 10 ug
$600.00
RG215174 GPR35 (tGFP-tagged) - Human G protein-coupled receptor 35 (GPR35), transcript variant 1 10 ug
$489.00 $500.00

file-alt Citations

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