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B7H3 (CD276) (NM_001024736) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG215064
CD276 (tGFP-tagged) - Human CD276 molecule (CD276), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $945.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol B7H3
Synonyms 4Ig-B7-H3; B7-H3; B7H3; B7RP-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001024736
ORF Size 1602 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001024736.2
RefSeq Size 3419 bp
RefSeq ORF 1605 bp
Locus ID 80381
UniProt ID Q5ZPR3
Cytogenetics 15q24.1
Protein Families Druggable Genome, Transmembrane
Protein Pathways Cell adhesion molecules (CAMs)
Summary The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2011
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"NM_001024736" in other vectors (4)
SKU Description Size Price
RC215064 CD276 (Myc-DDK-tagged)-Human CD276 molecule (CD276), transcript variant 1 10 ug
$289.00 $745.00
RC215064L3 Lenti-ORF clone of CD276 (Myc-DDK-tagged)-Human CD276 molecule (CD276), transcript variant 1 10 ug
$1,045.00
RC215064L4 Lenti-ORF clone of CD276 (mGFP-tagged)-Human CD276 molecule (CD276), transcript variant 1 10 ug
$1,045.00
SC302298 CD276 (untagged)-Human CD276 molecule (CD276), transcript variant 1 10 ug
$747.00

Plasmid Map for RG215064

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Cloning scheme for RG215064
Circular map for RG215064

file-alt Citations

B7H3 regulates differentiation and serves as a potential biomarker and theranostic target for human glioblastoma
Zhang, Wang, Marzese et al
Lab. Invest. (2019)
AI Summary: OriGene's B7H3 expression plasmids were used to overexpress B7H3 in U138 glioblastoma cells, enabling the study of B7H3's role in cell differentiation and tumor growth in this research.
Snippet: B7H3 expression plasmids (Origene, Rockville, MD) were delivered into U138 cells (which have low expression of B7H3) with JetPrime transfection agent (Polyplus, Berkeley, CA) for 6 h, then successfully transfected cells were selected using 800 μg/mL geneticin
B7-H3 associated with tumor progression and epigenetic regulatory activity in cutaneous melanoma
Wang, Chong, Nakamura et al
J. Invest. Dermatol. (2013) 133 (8), 2050-8 DOI: 10.1038/jid.2013.114
Cited by 138
AI Summary: OriGene's human B7-H3 cDNA vector was used in this study to overexpress B7-H3 in melanoma cells, enabling the investigation of its role in tumor progression and metastasis.
Snippet: Melanoma cell lines (M-1, M-101, M-111, M-12, M-14, JK-0346 Mel-B, JH-1173, Wm266-4) were cultured in RPMI 1640 (Life Technologies, Grand Island, NY) as previously described (Goto ... The human B7-H3 cDNA vector was purchased from Origene Company (Rockville, MD).
Showing 1-2 of 2 papers.
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