LCTL (NM_207338) Human Tagged ORF Clone

  • TrueORF®
SKU
RG213528
LCTL (tGFP-tagged) - Human lactase-like (LCTL)
  $991.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LCTL
Synonyms KLG; KLPH
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG213528 representing NM_207338
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCCAGTGTGGGTCGCCACCCTTCTGTGGATGCTACTGCTGGTGCCCAGGCTGGGGGCCGCCCGGA
AGGGGTCCCCAGAAGAGGCCTCCTTCTACTATGGAACCTTCCCTCTTGGCTTCTCCTGGGGCGTGGGCAG
TTCTGCCTACCAGACGGAGGGCGCCTGGGACCAGGACGGGAAAGGGCCTAGCATCTGGGACGTCTTCACA
CACAGTGGGAAGGGGAAAGTGCTTGGGAATGAGACGGCAGATGTAGCCTGTGACGGCTACTACAAGGTCC
AGGAGGACATCATTCTGCTGAGGGAACTGCACGTCAACCACTACCGATTCTCCCTGTCTTGGCCCCGGCT
CCTGCCCACAGGCATCCGAGCCGAGCAGGTGAACAAGAAGGGAATCGAATTCTACAGTGATCTTATCGAT
GCCCTTCTGAGCAGCAACATCACTCCCATCGTGACCTTGCACCACTGGGATCTGCCACAGCTGCTCCAGG
TCAAATACGGTGGGTGGCAGAATGTGAGCATGGCCAACTACTTCAGAGACTACGCCAACCTGTGCTTTGA
GGCCTTTGGGGACCGTGTGAAGCACTGGATCACGTTCAGTGATCCTCGGGCAATGGCAGAAAAAGGCTAT
GAGACGGGCCACCATGCGCCGGGCCTGAAGCTCCGCGGCACCGGCCTGTACAAGGCAGCACACCACATCA
TTAAGGCCCACGCCAAAGCCTGGCATTCTTATAACACCACGTGGCGCAGCAAGCAGCAAGGTCTGGTGGG
AATTTCATTGAACTGTGACTGGGGGGAACCTGTGGACATTAGTAACCCCAAGGACCTAGAGGCTGCCGAG
AGATACCTACAGTTCTGTCTGGGCTGGTTTGCCAACCCCATTTATGCCGGTGACTACCCCCAAGTCATGA
AGGACTACATTGGAAGAAAGAGTGCAGAGCAAGGCCTGGAGATGTCGAGGTTACCGGTGTTCTCACTCCA
GGAGAAGAGCTACATTAAAGGCACATCCGATTTCTTGGGATTAGGTCATTTTACTACTCGGTACATCACG
GAAAGGAACTACCCCTCCCGCCAGGGGCCCAGCTACCAGAACGATCGTGACTTGATAGAGCTGGTTGACC
CAAACTGGCCAGATCTGGGGTCTAAATGGCTATATTCTGTGCCATGGGGATTTAGGAGGCTCCTTAACTT
TGCTCAGACTCAATACGGTGATCCTCCCATATATGTGATGGAAAATGGAGCATCTCAAAAATTCCACTGT
ACTCAATTATGTGATGAGTGGAGAATTCAATACCTTAAAGGATACATAAATGAAATGCTAAAAGCTATAA
AAGATGGTGCTAATATAAAGGGGTATACTTCCTGGTCTCTGTTGGATAAGTTTGAATGGGAGAAAGGATA
CTCAGATAGATATGGATTCTACTATGTTGAATTTAACGACAGAAATAAGCCTCGCTATCCAAAGGCTTCA
GTTCAATATTACAAGAAGATTATCATTGCCAATGGGTTTCCCAATCCAAGAGAGGTGGAAAGTTGGTACC
TCAAAGCTTTGGAAACTTGCTCTATCAACAATCAGATGCTTGCTGCAGAGCCCTTGCTAAGTCACATGCA
AATGGTTACGGAGATCGTGGTACCCACTGTCTGCTCCCTCTGTGTCCTCATCACTGCTGTTCTACTAATG
CTCCTCCTGAGGAGGCAGAGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG213528 representing NM_207338
Red=Cloning site Green=Tags(s)

MKPVWVATLLWMLLLVPRLGAARKGSPEEASFYYGTFPLGFSWGVGSSAYQTEGAWDQDGKGPSIWDVFT
HSGKGKVLGNETADVACDGYYKVQEDIILLRELHVNHYRFSLSWPRLLPTGIRAEQVNKKGIEFYSDLID
ALLSSNITPIVTLHHWDLPQLLQVKYGGWQNVSMANYFRDYANLCFEAFGDRVKHWITFSDPRAMAEKGY
ETGHHAPGLKLRGTGLYKAAHHIIKAHAKAWHSYNTTWRSKQQGLVGISLNCDWGEPVDISNPKDLEAAE
RYLQFCLGWFANPIYAGDYPQVMKDYIGRKSAEQGLEMSRLPVFSLQEKSYIKGTSDFLGLGHFTTRYIT
ERNYPSRQGPSYQNDRDLIELVDPNWPDLGSKWLYSVPWGFRRLLNFAQTQYGDPPIYVMENGASQKFHC
TQLCDEWRIQYLKGYINEMLKAIKDGANIKGYTSWSLLDKFEWEKGYSDRYGFYYVEFNDRNKPRYPKAS
VQYYKKIIIANGFPNPREVESWYLKALETCSINNQMLAAEPLLSHMQMVTEIVVPTVCSLCVLITAVLLM
LLLRRQS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_207338
ORF Size 1701 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_207338.4
RefSeq Size 2128 bp
RefSeq ORF 1704 bp
Locus ID 197021
UniProt ID Q6UWM7
Cytogenetics 15q22.31
Protein Families Transmembrane
Summary This gene encodes a member of family 1 glycosidases. Glycosidases are enzymes that hydrolyze glycosidic bonds and are classified into families based on primary amino acid sequence. Most members of family 1 have two conserved glutamic acid residues, which are required for enzymatic activity. The mouse ortholog of this protein has been characterized and has a domain structure of an N-terminal signal peptide, glycosidase domain, transmembrane domain, and a short cytoplasmic tail. It lacks one of the conserved glutamic acid residues important for catalysis, and its function remains to be determined (PMID: 12084582). Alternative splicing results in multiple transcript variants. provided by RefSeq, Jun 2013
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Documents
Resources
"NM_207338" in other vectors (6)
SKU Description Size Price
RC213528 LCTL (Myc-DDK-tagged)-Human lactase-like (LCTL) 10 ug
$791.00
RC213528L1 Lenti ORF clone of Human lactase-like (LCTL), Myc-DDK-tagged 10 ug
$1,091.00
RC213528L2 Lenti ORF clone of Human lactase-like (LCTL), mGFP tagged 10 ug
$1,091.00
RC213528L3 Lenti ORF clone of Human lactase-like (LCTL), Myc-DDK-tagged 10 ug
$1,091.00
RC213528L4 Lenti ORF clone of Human lactase-like (LCTL), mGFP tagged 10 ug
$1,091.00
SC308461 LCTL (untagged)-Human lactase-like (LCTL) 10 ug
$793.00

Plasmid Map for RG213528

Download sequence for

Cloning scheme for RG213528

Circular map for RG213528

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